LMB-1 producing #Citrobacter freundii from #Argentina, a novel player in the field of MBLs (Int J Antimicrob Agents, abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Int J Antimicrob Agents. 2019 Nov 27. pii: S0924-8579(19)30328-0. doi: 10.1016/j.ijantimicag.2019.11.014. [Epub ahead of print]

LMB-1 producing Citrobacter freundii from Argentina, a novel player in the field of MBLs.

Dabos L1, Rodriguez CH2, Nastro M2, Dortet L3, Bonnin R4, Famiglietti A2, Iorga BI5, Vay C2, Naas T6.

Author information: 1 EA7361 “Structure, dynamic, function and expression of broad spectrum β-lactamases”, Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France; Joint research Unit EERA « Evolution and Ecology of Resistance to Antibiotics », Institut Pasteur-APHP-University Paris Sud, Paris, France. 2 Departamento de Bioquímica Clinica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina. 3 EA7361 “Structure, dynamic, function and expression of broad spectrum β-lactamases”, Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France; Joint research Unit EERA « Evolution and Ecology of Resistance to Antibiotics », Institut Pasteur-APHP-University Paris Sud, Paris, France; Department of Bacteriology-Hygiene, Bicêtre Hospital, APHP, Le Kremlin-Bicêtre, France; French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France. 4 EA7361 “Structure, dynamic, function and expression of broad spectrum β-lactamases”, Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France; Joint research Unit EERA « Evolution and Ecology of Resistance to Antibiotics », Institut Pasteur-APHP-University Paris Sud, Paris, France; French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France. 5 CNRS, UMR3525, Paris, France. 6 EA7361 “Structure, dynamic, function and expression of broad spectrum β-lactamases”, Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France; Joint research Unit EERA « Evolution and Ecology of Resistance to Antibiotics », Institut Pasteur-APHP-University Paris Sud, Paris, France; Department of Bacteriology-Hygiene, Bicêtre Hospital, APHP, Le Kremlin-Bicêtre, France; French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France. Electronic address: thierry.naas@aphp.fr.

 

Abstract

Carbapenemase-producing Enterobacterales expressing OXA-48, KPC, NDM, VIM or IMP enzymes are increasingly reported worldwide. We have characterized LMB-1, a novel metallo-β-latamase (MBL) of Ambler class B3 from Citrobacter freundii 164 (Cf164) clinical isolate from Buenos Aires, Argentina. Cf164 displayed reduced susceptibility to carbapenems but gave inconsistent results with carbapenemase confirmatory tests, suggesting the presence of a weak carbapenemase. Analysis of WGS of Cf164 using Resfinder revealed four β-lactamase genes coding for CTX-M-8, PER-2, TEM-1 and CMY-150, a novel chromosomally-encoded CMY variant. Kinetic parameters of purified CMY-150 did not reveal any carbapenemase activity. However, CMY-150 conferred to E. coli higher MIC values for ceftazidime and aztreonam as compared to CMY-2. The in-house developed β-lactamase search software (ResMINER) in WGS data, revealed a novel subclass B3 MBL named LMB-1. LMB-1 conferred to E. coli, resistance to penicillins, to expanded-spectrum cephalosporins and reduced susceptibility to carbapenems. The blaLMB-1 gene was located on a 176-kb IncA/C2 plasmid. LMB-1 shared 99% of amino acid sequence identity with the MBL encoded in the chromosome of Rheinheimera pacifica, it’s likely progenitor. Despite repeated attempts, LMB-1 could not be purified, thus only specific activities could evidence hydrolysis of carbapenems. Here we report CMY-150, a novel CMY-2 variant that confers increased ceftazidime and aztreonam MICs to E. coli and the first description of LMB-1 in Argentina. This work underlines the need for several CPE confirmatory tests, as this novel enzyme might have been missed using only one.

Copyright © 2019 Elsevier Ltd. All rights reserved.

KEYWORDS: CPE; Carbapenemase; Class B3 MBL; Metallo-beta-lactamase

PMID: 31785341 DOI: 10.1016/j.ijantimicag.2019.11.014

Keywords: Antibiotics; Drugs Resistance; E. Coli; Citrobacter freundii; Carbapenem; Beta-lactams; Argentina.

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#Congenital #Zika #syndrome in #Argentina: case series study (Arch Argent Pediatr., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Arch Argent Pediatr. 2019 Dec 1;117(6):e635-e639. doi: 10.5546/aap.2019.e635.

[Congenital Zika syndrome in Argentina: case series study].

[Article in Spanish]

Pastrana A1, Albarracín M2, Hoffmann M3, Delturco G3, López R3, Gil R3, Guzmán A3, Del Barco M2, Espeche A3.

Author information: 1 Servicio de Neurología, Hospital Público Materno Infantil de Salta, Argentina. analia.pastrana@gmail.com. 2 Servicio de Neonatología, Hospital Público Materno Infantil de Salta, Argentina. 3 Servicio de Neurología, Hospital Público Materno Infantil de Salta, Argentina.

 

Abstract

In 2015, there was an increase in the incidence of congenital microcephaly in newborns in Brazil. Months later, the causal relationship between Zika virus and these findings was discovered. In Argentina, during the first outbreak there were 5 cases of congenital Zika syndrome reported. In 2017, there was a new outbreak which involved Salta province. We describe 2 patients with autochthonous congenital Zika syndrome: one of the babies with severe congenital microcephaly with lissencephaly, calcifications and ventriculomegaly; and another baby with postnatal microcephaly with asymmetric polymicrogyria, calcifications and delayed myelination. The real impact of this disease is still uncertain, so it is necessary an adequate multidisciplinary monitoring of patients exposed to Zika virus to better understand the infection and its natural history.

Sociedad Argentina de Pediatría.

KEYWORDS: Zika virus; congenital Zika syndrome; microcephaly

PMID: 31758900 DOI: 10.5546/aap.2019.e635

Keywords: Zika Virus; Zika Congenital Syndrome; Microcephaly; Pediatrics; Argentina.

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Relevance of #HTLV1 proviral load in asymptomatic and symptomatic #patients living in #endemic and non-endemic areas of #Argentina (PLOS One, abstract)

[Source: PLOS One, full page: (LINK). Abstract, edited.]

OPEN ACCESS /  PEER-REVIEWED / RESEARCH ARTICLE

Relevance of HTLV-1 proviral load in asymptomatic and symptomatic patients living in endemic and non-endemic areas of Argentina

María Verónica Pineda, María Belén Bouzas, Mirta Remesar, Ariel Fridman, Carlos Remondegui, Lilia Mammana, Natalia Altamirano, Patricia Paradiso, Patricia Costantini, Luciana Tadey, Paula Aulicino, Andrea Mangano

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Published: November 22, 2019 / DOI: https://doi.org/10.1371/journal.pone.0225596

 

Abstract

HTLV-1 proviral load (pVL) in peripheral blood mononuclear cell (PBMCs) is proposed as a marker of disease progression but its role still remains controversial. The aim of this study was to evaluate the levels of HTLV-1 pVL in symptomatic patients and asymptomatic HTLV-1 carriers. In this cross-sectional study the pVL was measured by Real Time PCR in 102 asymptomatic carriers and 22 symptomatic patients (5ATLL, 15 TSP and 2 uveitis). We observed that the HTLV-1 pVL was significantly higher in symptomatic patients (median = 4.99 log10 HTLV-1 copies /106 PBMCs) compared to asymptomatic HTLV-1 carriers (median = 4.38 log10 HTLV-1 copies /106 PBMCs; p = 0.0030). A wide variation on the HTLV-1 pVL levels among asymptomatic HTLV-1 carriers was observed with some pVL as high as those observed in symptomatic patients. The asymptomatic HTLV-1 carriers were divided according to the place of birth and the highest levels of pVL were detected among patients from endemics areas from the North of Argentina. Our results reinforce the usefulness of the proviral load would be a prognostic marker of HTLV-1 disease progression. Moreover, host, viral or socio-environmental factors cannot be excluded as determinant of high proviral load.

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Citation: Pineda MV, Bouzas MB, Remesar M, Fridman A, Remondegui C, Mammana L, et al. (2019) Relevance of HTLV-1 proviral load in asymptomatic and symptomatic patients living in endemic and non-endemic areas of Argentina. PLoS ONE 14(11): e0225596. https://doi.org/10.1371/journal.pone.0225596

Editor: Eduardo Anguita, Hospital Clinico Universitario San Carlos, SPAIN

Received: April 1, 2019; Accepted: October 30, 2019; Published: November 22, 2019

Copyright: © 2019 Pineda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: This work was supported by Fondo Nacional para Ciencia y Tecnología (FONCYT) [grant number PICT 2010-0502], http://www.agencia.mincyt.gob.ar/foncyt.php. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Keywords: Retrovirus; HTLV-1; Argentina.

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#Neisseria meningitidis isolated from #patients in #MSMs (Rev Argent Microbiol., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Rev Argent Microbiol. 2019 Oct 15. pii: S0325-7541(19)30079-3. doi: 10.1016/j.ram.2019.03.009. [Epub ahead of print]

[Neisseria meningitidis isolated from patients in men who have sex with men].

[Article in Spanish]

García SD1, Sorhuet-Pereira C2, Perazzi BE3, Losada ME3, Cabellos Astorga G3, Casco RH4, Vay CA3, Mollerach ME5, Famiglietti ÁMR3.

Author information: 1 Cátedra de Microbiología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina. Electronic address: biosgarcia@yahoo.com.ar. 2 Cátedra de Microbiología, Departamento de Microbiología, Inmunología y Genética, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina. 3 Cátedra de Microbiología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina. 4 Programa de ETS, Hospital de Clínicas, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina. 5 Cátedra de Microbiología, Departamento de Microbiología, Inmunología y Genética, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina; CONICET, Buenos Aires, Argentina.

 

Abstract

During the periods 2000-2004 and 2014-2015, Neisseria meningitidis was investigated in men who have sex with men, 1143 and 544 respectively, who consulted in the sexually-transmitted disease program. Prevalence, serogroup distribution and susceptibility to antibiotics were determined. Pharyngeal, rectal and urethral swabs were cultivated on selective Thayer-Martin modified medium. The identification was performed by biochemical tests and mass spectrometry by MALDI-TOF. Serogroups B, C, W and Y were investigated by PCR in 85 isolates recovered from the pharynx belonging to the second period. MICs of penicillin, ceftriaxone, rifampicin, azithromycin and ciprofloxacin were determined for 66 and 102 isolates from periods 1 and 2 respectively, according to CLSI. The prevalence of N. meningitidis was 17.8% and 28.1%, in periods 1 and 2 respectively; the isolates were mainly recovered from the pharynx. The distribution of serogroups was B 31.5%; Y 7.6%; W 3.3% and 9.8% non-capsulated and the rest would belong to other serogroups. Isolates classified as intermediate to penicillin were 34.8% and 63.7% (first and second periods, respectively); moreover, 11.8% of the isolates from the second period were resistant. All isolates were susceptible to ceftriaxone, to ciprofloxacin (except 3 isolates with MIC values between 0.25 and 0.5μg/ml), 3% were resistant to rifampicin and 2% were not susceptible to azithromicin. The prevalence of N. meningitidis carriage in men who have sex with men was high with a high rate of penicillin non-susceptible isolates. B was the prevalent serogroup.

Copyright © 2019 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

KEYWORDS: Neisseria meningitidis; Resistance; Resistencia; Serogroup; Serogrupo

PMID: 31628000 DOI: 10.1016/j.ram.2019.03.009

Keywords: Antibiotics; Drugs Resistance; Penicillin; Neisseria meningitidis; Argentina.

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#Vector competence of #Aedes aegypti for different strains of #Zika virus in #Argentina (PLoS Negl Trop Dis., abstract)

[Source: PLoS Neglected Tropical Diseases, full page: (LINK). Abstract, edited.]

OPEN ACCESS /  PEER-REVIEWED / RESEARCH ARTICLE

Vector competence of Aedes aegypti for different strains of Zika virus in Argentina

Melisa Berenice Bonica  , Silvina Goenaga  , María Laura Martin, Mariel Feroci, Victoria Luppo, Evangelina Muttis, Cintia Fabbri, María Alejandra Morales, Delia Enria, María Victoria Micieli , Silvana Levis

Published: June 12, 2019 / DOI: https://doi.org/10.1371/journal.pntd.0007433

 

Abstract

The importance of Zika virus (ZIKV) has increased noticeably since the outbreak in the Americas in 2015, when the illness was associated with congenital disorders. Although there is evidence of sexual transmission of the virus, the mosquito Aedes aegypti is believed to be the main vector for transmission to humans. This species of mosquito has not only been found naturally infected with ZIKV, but also has been the subject of study in many vector competence assays that employ different strains of ZIKV around the world. In Argentina, the first case was reported in February 2016 and a total of 278 autochthonous cases have since been confirmed, however, ZIKV virus has not been isolated from any mosquito species yet in Argentina. In order to elucidate if Argentinian Ae. aegypti populations could be a possible vector of ZIKV, we conducted vector competence studies that involved a local strain of ZIKV from Chaco province, and a Venezuelan strain obtained from an imported case. For this purpose, Ae. aegypti adults from the temperate area of Argentina (Buenos Aires province) were fed with infected blood. Body, legs and saliva were harvested and tested by plaque titration on plates of Vero cells for ZIKV at 7, 11 and 14 days post infection (DPI) in order to calculate infection, transmission, and dissemination rates, respectively. Both strains were able to infect mosquitoes at all DPIs, whereas dissemination and transmission were observed at all DPIs for the Argentinian strain but only at 14 DPI for the Venezuelan strain. This study proves the ability of Ae. aegypti mosquitoes from Argentina to become infected with two different strains of ZIKV, both belonging to the Asian lineage, and that the virus can disseminate to the legs and salivary glands.

 

Author summary

Zika virus is a flavivirus transmitted by mosquitoes, isolated for the first time in the Ziika Forest in Uganda in 1947 from a rhesus macaque monkey. The disease is usually asymptomatic, but sometimes it causes a mild illness that comes with fever, rash, joint pain, and conjunctivitis. The World Health Organization focused the attention on this virus after the outbreak in the Americas, when the virus was linked to microcephaly and serious neurological diseases, including Guillain-Barré syndrome. Aedes aegypti was incriminated as the main vector of the virus as it was found both naturally and experimentally infected. This mosquito species was declared eradicated in Argentina by 1970 but re-emerged in 1989. Recent studies found a peculiarity in the genetics of Argentinian Ae. aegypti populations that consists in a combination between both subspecies: Ae. aegypti formosus and Ae. aegypti aegypti. Our study tries to elucidate if Ae. aegypti from Argentina are able to transmit the virus in order to add these mosquitoes to the list of possible vectors of ZIKV and, in future prospect, orient to fight the virus by controlling the vector.

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Citation: Bonica MB, Goenaga S, Martin ML, Feroci M, Luppo V, Muttis E, et al. (2019) Vector competence of Aedes aegypti for different strains of Zika virus in Argentina. PLoS Negl Trop Dis 13(6): e0007433. https://doi.org/10.1371/journal.pntd.0007433

Editor: Paulo Pimenta, Fundaçao Oswaldo Cruz, BRAZIL

Received: December 11, 2018; Accepted: April 15, 2019; Published: June 12, 2019

Copyright: © 2019 Bonica et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the manuscript.

Funding: MBB, EM and MVM received funds from the National Agency for the Promotion of Science and Technology of Argentina (ANPCYT) (PICT 2015-0665). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Keywords: Zika Virus; Mosquitoes; Aedes aegypti; Argentina.

——

#Hepatitis A #outbreak affecting men who have sex with men (#MSM) in central #Argentina, occurred in July 2017-April 2018, later than the #European outbreak (J Clin Virol., abstract)

[Source: Journal of Clinical Virology, full page: (LINK). Abstract, edited.]

Journal of Clinical Virology / Available online 31 May 2019 / In Press, Accepted Manuscript

Hepatitis A outbreak affecting men who have sex with men (MSM) in central Argentina, occurred in July 2017-April 2018, later than the European outbreak

Jorge Mariojouls a, Gonzalo Castro a, María Belén Pisano b, Paula Barbero c, Anabella Fantilli b, Mariel Borda a, Fernando Canna a, Gabriela Barbás a, Viviana Ré b

{a} Laboratorio Central, Ministerio de Salud de la Provincia de Córdoba, Argentina; {b}
Instituto de Virología “Dr. J.M.Vanella”, Facultad de Ciencias Médicas, CONICET, Universidad Nacional de Córdoba, Argentina; {c} Area Epidemiología, Ministerio de Salud de la Provincia de Córdoba, Argentina

Received 7 March 2019, Revised 17 May 2019, Accepted 30 May 2019, Available online 31 May 2019. DOI: https://doi.org/10.1016/j.jcv.2019.05.014

 

Highlights

  • A Hepatitis A outbreak among young adult MSM occurred in 2017-2018 in Argentina.
  • HAV genotype IA, strain VRD 521–2016, was the responsible of the outbreak.
  • Reinforce official policy of vaccination in MSM is mandatory.

 

Abstract

Background

During June-2016 – May-2017, several outbreaks of HA were recorded in Europe, especially described in MSM. In our area since July-2017, an increase of hepatitis A (HA) notification was reported.

Objective

In order to understand the unusual increase of cases occurred in the central region of Argentina, the aim of this study was to describe, characterize and contextualize epidemiologically the HA outbreak occurred this area, until April2018.

Study design

HA cases (positive anti-HAV IgM) obtained from the calendar week 29/2017 in which the first case of MSM was recognized were included in our study. HAV RNA detection and molecular characterization was performed from serum samples and / or stool by RT – PCR of VP1 / 2A genomic region (360bp).

Results

Of the 32 cases notified, 87.5% of them were unvaccinated men and 69.6% were MSM (mean age 31.9 years). All MSM associated HAV sequences were genotyped as IA, and clustered with the VRD 521-2016 strain, responsible of causing outbreaks mostly in MSM in Europe since mid-2016.

Conclusion

As a consequence of the implementation of immunization in children, and the improvement in socio-economic, hygienic and sanitation factors, young adults are becoming increasingly susceptible to HAV infections. Here we add evidence in South America to the HA outbreaks described worldwide among young MSM, demonstrating the need to reinforce official policy of vaccination, in this group and adjust epidemiological surveillance, catch-up vaccination for adolescents, young adults and immunosuppressed patients.

Keywords: Hepatitis A – HAV surveillance – outbreak – MSM – genotyping – Argentina

© 2019 Published by Elsevier B.V.

Keywords: Hepatitis A; Argentina.

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#SME-4-producing #Serratia marcescens from #Argentina belonging to clade 2 of the S. marcescens phylogeny (J Antimicrob Chemother., abstract)

[Source: Journal of Antimicrobial Chemotherapy, full page: (LINK). Abstract, edited.]

SME-4-producing Serratia marcescens from Argentina belonging to clade 2 of the S. marcescens phylogeny

Laura Dabos, Rafael Patiño-Navarrete, Marcela Nastro, Angela Famiglietti, Philippe Glaser, Carlos H Rodriguez, Thierry Naas

Journal of Antimicrobial Chemotherapy, dkz115, https://doi.org/10.1093/jac/dkz115

Published: 16 April 2019

 

Abstract

Background

SME carbapenemases are increasingly reported, especially from North and South America. Here, we describe an SME-4-producing Serratia marcescens(SME-Sm) clinical isolate from Argentina and compare its genome with other SME-Sm and Sm isolates recovered from public databases.

Methods

Sm isolates were characterized by WGS using Illumina technology, susceptibility testing and MIC determination. Carbapenemase activity was revealed by biochemical tests based on imipenem hydrolysis. A whole-genome phylogeny was estimated for all the Sm isolates retrieved from public databases with kSNP3 and a whole-genome phylogenetic analysis based on non-recombinant core SNPs was inferred for Sm complete genomes and for those encoding any blaSME variants.

Results

Sm163 was resistant to amoxicillin, temocillin, aztreonam and carbapenems, remaining susceptible to extended-spectrum cephalosporins. WGS analysis of Sm163 revealed a genome of 5 139 329 bp and a chromosomally encoded blaSME-4 carbapenemase gene located on a genomic island closely related to SmarGI1-1 of Sm N11-02820. Comparison of the Sm genomes revealed that the 14 SME-Sm isolates possess this genomic island inserted at the same loci, that 13/14 belong to clade 1 and that 11/14 form a well-defined subcluster of cluster I of Sm clade 1, while Sm163 belongs to clade 2, suggesting that an SME-encoding genomic island may have been transferred between isolates from different clades.

Conclusions

To the best of our knowledge this is the first report of an SME-4-encoding Smfrom Argentina. The blaSME-4 gene is located on a SmarGI1-1-like genomic island. The genome of Sm163 belongs to clade 2, unlike all the other SME-Smisolates, which belong to clade 1.

Issue Section: ORIGINAL RESEARCH

© The Author(s) 2019. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Keywords: Antibiotics; Drugs Resistance; Carbapene; Serratia marcescens; Amoxicillin; Temocillin; Aztreonam; Argentina.

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