[Source: Virologica Sinica, full page: (LINK). Abstract, edited.]
Cross-sectional Seroprevalence and Genotype of Hepatitis E Virus in Humans and Swine in a High-density Pig-farming Area in Central China
Authors: Yilin Shu, Yameng Chen, Sheng Zhou, Shoude Zhang, Qin Wan, Changcai Zhu, Zhijiang Zhang, Hailong Wu, Jianbo Zhan, Ling Zhang
RESEARCH ARTICLE / First Online: 01 July 2019
Hepatitis E virus (HEV) infection is a common public health problem in developing countries. However, the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farming areas in central China are still inadequately understood. Here, cross-sectional serological and genotypic surveys of HEV among the 1232 general population, 273 workers occupationally exposed to swine, and 276 pigs in a high-density pig-breeding area, were undertaken by ELISA and nested RT-PCR methods. Anti-HEV IgG was detected in 26.22% of general population and 48.35% of occupational workers. The prevalence of swine serum HEV-Ag was 6.52%. The prevalence of anti-HEV IgG was significantly higher among the workers occupationally exposed to swine than among the general population. An increased HEV seropositivity risk among the general population was associated with either being a peasant or male and was very strongly associated with the increase of age. Among the occupationally exposed group, the prevalence of anti-HEV IgG antibodies increased with age and working years. Among the 30 HEV-IgM-positive people, the infection rates of clerks in the public, peasants, pork retailers, and pig farmers were higher than those of others. A phylogenetic analysis revealed that all the isolates belonged to subgenotype 4d, and four people and four pigs shared 97.04%–100% sequence homology. This study revealed a high HEV seroprevalence among the general population and workers occupationally exposed to swine in the Anlu City, and supports the notion that swine are a source of human HEV infection.
Keywords: Hepatitis E virus (HEV) – Seroepidemiological study – Zoonosis – Cross-sectional study – Genotype
Yilin Shu, Yameng Chen and Sheng Zhou authors contributed equally to this work.
Electronic supplementary material: The online version of this article ( https://doi.org/10.1007/s12250-019-00136-x) contains supplementary material, which is available to authorized users.
The authors would like to thank the Anlu animal husbandry and veterinary bureau for these supporting information on pig density at Anlu city in the central China in 2016. This work was partly supported by General Projects of Health and Family Planning Commission of Hubei Province of China no. WJ2017M174, and WJ2017M240 and Occupational Hazard and Identification Control of Hubei Provincial Key Laboratory Open Fund, no. OCHI2017G02.
YS, JZ, LZ and HW designed the study. YS, YC, SZ and QW performed the experiments. YS, SZ, CZ and ZZ analyzed the data. HC, YS and LZ drafted the manuscript. All authors read and approved the final manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Animal and Human Rights Statement
All institutional and national guidelines for the care and use of animals were followed. Additional informed consent was obtained from all patients for which identifying information is included in this article.
Keywords: Hepatitis E; Pigs; Human; China; Seroprevalence.