[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Infect Genet Evol. 2020 Jan 6:104180. doi: 10.1016/j.meegid.2020.104180. [Epub ahead of print]
Sequencing of ZIKV genomes directly from Ae. aegypti and Cx. quinquefasciatus mosquitoes collected during the 2015-16 epidemics in Recife.
Paiva MHS1, Guedes DRD2, Krokovsky L2, Machado LC2, Rezende TMT2, de Morais Sobral MC2, Ayres CFJ2, Wallau GL3.
Author information: 1 Universidade Federal de Pernambuco, Caruaru, Brazil. 2 Entomology Department of the Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil. 3 Entomology Department of the Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil. Electronic address: email@example.com.
Zika virus (ZIKV) is a negative sense RNA virus from the Flaviviridae family, which was relatively unknown until the first human epidemic in Micronesia, in 2007. Since its spread to French Polynesia and the Americas. Recife, the capital of Pernambuco state and epicenter of the Zika epidemic in Brazil, experienced a large number of microcephaly cases and other congenital abnormalities associated to the ZIKV infection from, 2015 to 16. Evidences suggest that both Aedes aegypti and Culex quinquefasciatus mosquitoes from Recife are capable of replicating and transmitting the virus. Here, we conducted high throughput sequencing of ZIKV genomes directly from Ae. aegypti and Cx. quinquefasciatus mosquitoes collected during the ZIKV epidemics in Recife, in order to investigate the variability and evolution of the virus. We obtained 11 draft ZIKV genomes derived from 5 pools from each Ae. aegypti and Cx. quinquefasciatus species. Genome coverage breadth ranged from 16 to 100% and average depth from 45 to 46,584×. Two of these genomes were obtained from pools of unfed Cx. quinquefasciatus females. Amino acid substitutions found here were not species-specific, which could indicate species specific virus adaptation. In addition, molecular clock dating estimated that ZIKV draft genomes obtained here were co-circulating in the region during the epidemics. Overall results highlight that viral mutations and even minor variants can be detected in genomes directly sequenced from mosquito samples and insights about natural viral genomic variability and viral evolution can be useful when designing tools for mosquito control programs.
Copyright © 2019. Published by Elsevier B.V.
KEYWORDS: Aedes aegypti; Arboviruses; Culex quinquefasciatus; Surveillance; ZIKV
PMID: 31918041 DOI: 10.1016/j.meegid.2020.104180
Keywords: Zika Virus; Aedes aegypti; Culex quinquefascitus; Mosquitoes; Brazil.