#Chikungunya, a #risk #factor for #GBS (Clin Infect Dis., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Clin Infect Dis. 2019 Jul 9. pii: ciz625. doi: 10.1093/cid/ciz625. [Epub ahead of print]

Chikungunya, a risk factor for Guillain-Barré syndrome.

Stegmann-Planchard S1,2, Gallian P3,4, Tressières B2, Leparc-Goffart I5, Lannuzel A6, Signaté A7, Laouénan C8, Cabié A9, Hoen B10.

Author information: 1 Unité de Maladies Infectieuses, Centre Hospitalier Louis-Constant Fleming, Saint Martin, France. 2 Centre d’Investigation Clinique Antilles Guyane, Inserm CIC 1424, Pointe-à-Pitre, Guadeloupe, France. 3 Etablissement Français du Sang, Saint Denis-La Plaine Stade de France, France. 4 Unité des Virus Emergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection), Marseille, France. 5 Centre national de référence des arbovirus, Errit-IRBA HIA Laveran, Marseille, France. 6 Service de Neurologie, Centre Hospitalier Universitaire de Pointe-à-Pitre, Guadeloupe, France. 7 Service de Neurologie, Centre Hospitalier Universitaire de Martinique, Fort de France, Martinique, France. 8 IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité ; Centre d’Investigation Clinique Inserm CIC-EC 1425 and Département d’Epidémiologie, Biostatistiques et recherche Clinique, Hôpital Bichat-Claude Bernard, AP-HP, all in Paris, France. 9 Centre d’Investigation Clinique Antilles Guyane, Inserm CIC 1424 and Service de Maladies Infectieuses et Tropicales, Centre Hospitalier Universitaire de la Martinique and Université des Antilles, EA 4537, all in Fort-de-France, France. 10 Centre d’Investigation Clinique Antilles Guyane, Inserm CIC 1424 and Service de Maladies Infectieuses et Tropicales, Dermatologie, Médecine Interne, Centre Hospitalier Universitaire de la Guadeloupe and Université des Antilles, EA 4537, all in Pointe-à-Pitre, France.

 

Abstract

In a matched case-control study where 24 cases developed Guillain-Barré syndrome (GBS) during the 2014 chikungunya outbreak in the French West Indies and 72 controls were blood donors who donated their blood at the same period, Chikungunya infection was a risk factor for GBS (OR 8.3, 95% CI 2.3-29.7, p=0.001).

© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

KEYWORDS: Chikungunya virus; Guillain-Barré syndrome; case-control study; chikungunya infection

PMID: 31290540 DOI: 10.1093/cid/ciz625

Keywords: Chikungunya fever; GBS.

——

Advertisements

#Chikungunya virus #vaccine candidates with decreased mutational robustness are attenuated in vivo and have compromised transmissibility (J Virol., abstract)

[Source: Journal of Virology, full page: (LINK). Abstract, edited.]

Chikungunya virus vaccine candidates with decreased mutational robustness are attenuated in vivo and have compromised transmissibility.

Lucía Carrau, Veronica V. Rezelj, María G. Noval, Laura I. Levi, Daniela Megrian, Herve Blanc, James Weger-Lucarelli, Gonzalo Moratorio, Kenneth Stapleford, Marco Vignuzzi

DOI: 10.1128/JVI.00775-19

 

ABSTRACT

Chikungunya virus (CHIKV) is a re-emerged arbovirus, member of the Togaviridae family. It circulates through mosquito vectors mainly of the Aedes family and a mammalian host. CHIKV causes chikungunya fever, a mild to severe disease characterized by arthralgia, with some fatal outcomes described. In the past years, several outbreaks mainly caused by enhanced adaptation of the virus to the vector and ineffective control of the contacts between infected mosquito populations and the human host have been reported. Vaccines represent the best solution for the control of insect-borne viruses, including CHIKV, but are often unavailable. We designed live attenuated CHIKV by applying a rational genomic design based on multiple replacements of synonymous codons. In doing so, the virus mutational robustness (capacity to maintain phenotype despite introduction of mutations to genotype) is decreased, driving the viral population towards deleterious evolutionary trajectories. When tested in the insect and mammalian hosts, we observed overall strong attenuation in both and greatly diminished signs of disease. Moreover, we found the vaccine candidates elicited protective immunity, related to the production of neutralizing antibodies after a single dose. During an experimental transmission cycle between mosquitoes and naïve mice, vaccine candidates could be transmitted by mosquito bite leading to asymptomatic infection in mice with compromised dissemination. Using deep sequencing technology we observed an increase in detrimental (stop) codons, which confirmed the effectiveness of this genomic design. Because the approach involves hundreds of synonymous modifications to the genome, the reversion risk is significantly reduced, rendering them promising vaccine candidates.

 

IMPORTANCE

Chikungunya fever is a debilitating disease that causes severe pain to the joints, which can compromise the patient’s lifestyle for several months and even in some grave cases lead to death. The etiological agent is chikungunya virus, an alphavirus transmitted by mosquito bite. Currently there are no approved vaccines or treatments against the disease. In our research, we developed novel live attenuated vaccine candidates against chikungunya virus by applying an innovative genomic design. When tested in the insect and mammalian host, the vaccine candidates did not cause disease, elicited strong protection against further infection and had low risk of reversion to pathogenic phenotypes.

Copyright © 2019 Carrau et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Keywords: Alphavirus; Togavirus; Chikungunya fever; Vaccines.

——

#Seroreactivity to #Chikungunya and #WNV Viruses in #Rwandan #Blood Donors (Vector Borne Zoo Dis., abstract)

[Source: Vector Borne and Zoonotic Diseases, full page: (LINK). Abstract, edited.]

Seroreactivity to Chikungunya and West Nile Viruses in Rwandan Blood Donors

Eric Seruyange, Karl Ljungberg, Claude Mambo Muvunyi, Jean Bosco Gahutu, Swaibu Katare, José Nyamusore, Yong-Dae Gwon, Magnus Evander, Heléne Norder, Peter Liljeström, and Tomas Bergström

Published Online: 27 Jun 2019

 

Abstract

Introduction:

Chikungunya virus (CHIKV) and West Nile virus (WNV) have previously been reported from several African countries, including those bordering Rwanda where they may have originated. However, there have been no serosurveillance reports from Rwanda regarding these two viral pathogens.

In this article, we present the first study of immunoglobulin G (IgG) seroreactivity of CHIKV and WNV in Rwandan blood donor samples.

Methods:

Blood donors from Rwanda (n = 874) and Sweden (n = 199) were tested for IgG reactivity against CHIKV, using an in-house enzyme-linked immunosorbent assay with the E1 envelope protein fused with p62 as antigen, and against WNV using a commercial kit. Data on mosquito distribution were obtained from the 2012 assessment of yellow fever virus circulation in Rwanda.

Results:

Seroreactivity to CHIKV was high in Rwanda (63.0%), when compared with Swedish donors, where only 8.5% were IgG positive. However, a cross-reactivity to O’nyong’nyong virus in neutralization test was noted in Rwandan donors. No significant difference in WNV seroreactivity was found (10.4% for Rwandan and 14.1% for Swedish donors). The relatively high seroreactivity to WNV among Swedish donors could partly be explained by cross-reactivity with tick-borne encephalitis virus prevalent in Sweden. Donors from the Eastern Province of Rwanda had the highest IgG reactivity to the two investigated viruses (86.7% for CHIKV and 33.3% for WNV). Five genera of mosquitoes were found in Rwanda where Culex was the most common (82.5%). The vector of CHIKV, Aedes, accounted for 9.6% of mosquitoes and this species was most commonly found in the Eastern Province.

Conclusions:

Our results showed high seroreactivity to CHIKV in Rwandan donors. The highest IgG reactivity to CHIKV, and to WNV, was found in the Eastern Province, the area reporting the highest number of mosquito vectors for these two viruses. Infection control by eliminating mosquito-breeding sites in population-dense areas is recommended, especially in eastern Rwanda.

Keywords: Arbovirus; Chikungunya virus; WNV; Serology; Seroprevalence; Rwanda.

—–

Sustained Low-Level #Transmission of #Zika and #Chikungunya Viruses after Emergence in the #Fiji Islands (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 25, Number 8—August 2019 / Dispatch

Sustained Low-Level Transmission of Zika and Chikungunya Viruses after Emergence in the Fiji Islands

Mike Kama1, Maite Aubry1, Taina Naivalu, Jessica Vanhomwegen, Teheipuaura Mariteragi-Helle, Anita Teissier, Tuterarii Paoaafaite, Stéphane Hué, Martin L. Hibberd, Jean-Claude Manuguerra, Ketan Christi, Conall H. Watson, Eric J. Nilles, John Aaskov, Colleen L. Lau, Didier Musso, Adam J. Kucharski1, and Van-Mai Cao-Lormeau1

Author affiliations: Fiji Centre for Communicable Disease Control, Suva, Fiji (M. Kama); The University of the South Pacific, Suva (M. Kama, T. Naivalu, K. Christi); Institut Louis Malardé, Papeete, Tahiti (M. Aubry, T. Mariteragi-Helle, A. Teissier, T. Paoaafaite, D. Musso, V.-M. Cao-Lormeau); Fiji National University, Suva (T. Naivalu); Institut Pasteur, Paris, France (J. Vanhomwegen, J.-C. Manuguerra); London School of Hygiene and Tropical Medicine, London, UK (S. Hué, M.L. Hibberd, C.H. Watson, A.J. Kucharski); World Health Organization, Suva (E.J. Nilles); Harvard Medical School and Brigham and Women’s Hospital, Boston, Massachusetts, USA (E.J. Nilles); Harvard Humanitarian Initiative, Cambridge, Massachusetts, USA (E.J. Nilles); Queensland University of Technology, Brisbane, Queensland, Australia (J. Aaskov); Australian National University, Canberra, Australian Capital Territory, Australia (C.L. Lau); Aix Marseille University, Marseille, France (D. Musso)

 

Abstract

Zika and chikungunya viruses were first detected in Fiji in 2015. Examining surveillance, phylogenetic, and serologic data, we found evidence of low-level transmission of Zika and chikungunya viruses during 2013–2017, in contrast to the major outbreaks caused by closely related virus strains in other Pacific Island countries.

Keywords: Zika Virus; Chikungunya fever; Fiji.

——

#Chikungunya Fever #Outbreak, #Zhejiang Province, #China, 2017 (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 25, Number 8—August 2019 / Research Letter

Chikungunya Fever Outbreak, Zhejiang Province, China, 2017

Junhang Pan1, Chunfu Fang1, Juying Yan, Hao Yan, Bingdong Zhan, Yi Sun, Ying Liu, Haiyan Mao, Guoping Cao, Lei Lv, Yanjun Zhang  , and Enfu Chen

Author affiliations: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China (J. Pan, J. Yan, H. Yan, Y. Sun, Y. Liu, H. Mao, Y. Zhang, E. Chen); Quzhou Center for Disease Control and Prevention, Quzhou, China (C. Fang, B. Zhan, G. Cao, L. Lv)

 

Abstract

We report a disease outbreak caused by chikungunya virus in Zhejiang Province, China, in August 2017. Phylogenic analysis indicated that this virus belonged to the Indian Ocean clade of the East/Central/South African genotype and was imported by a traveler returning from Bangladesh.

Keywords: Chikungunya fever; Zhejiang; China.

——

#GBS and its correlation with #dengue, #Zika and #chikungunya viruses #infection based on a literature review of reported cases in #Brazil (Acta Trop., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Acta Trop. 2019 Jun 17:105064. doi: 10.1016/j.actatropica.2019.105064. [Epub ahead of print]

Guillain-Barre Syndrome and its correlation with dengue, Zika and chikungunya viruses infection based on a literature review of reported cases in Brazil.

de Sousa Lima ME1, Rodrigues Bachur TP2, Frota Aragão G3.

Author information: 1 Curso de Medicina, Universidade Estadual do Ceará, Campus Itaperi, Av. Dr. Silas Munguba, 1700 – Itaperi, Fortaleza, CE, CEP 60.714-903, Brazil. Electronic address: matheus.eugenio@aluno.uece.br. 2 Curso de Medicina, Universidade Estadual do Ceará, Campus Itaperi, Av. Dr. Silas Munguba, 1700 – Itaperi, Fortaleza, CE, CEP 60.714-903, Brazil. Electronic address: tatiana.bachur@uece.br. 3 Curso de Medicina, Universidade Estadual do Ceará, Campus Itaperi, Av. Dr. Silas Munguba, 1700 – Itaperi, Fortaleza, CE, CEP 60.714-903, Brazil; Núcleo de Pesquisa e Desenvolvimento de Medicamentos, Faculdade de Medicina, Universidade Federal do Ceará, Campus do Porangabuçu, Rua Cel. Nunes de Melo, 1000 – Rodolfo Teófilo, Fortaleza, CE, CEP 60.430-275, Brazil. Electronic address: gislei.frota@uece.br.

 

Abstract

Guillain-Barre syndrome (GBS) is one of the main neurologic manifestations of arboviruses, especially Zika virus infection. As known, the prevalence of these diseases is high, so the risk of having an increase on GBS is relevant. The study purposes making a comparative survey between the involvement of dengue, Zika and chikungunya infections in the development of the GBS in Brazil, as well as search in literature resemblances and distinctions between beforehand reported cases. It was performed an electronic search in online databases, with articles published between the years of 2004 to 2018. A total of 729 articles about the proposed search were found, and 10 were selected according to inclusion and exclusion criteria. The medium age found in Brazilian studies was 42,9. The time lapse for the neurological symptoms manifest was 6,5 to 11 days. Facial palsy, paresthesia and member weakness were the main symptoms related. Pediatric cases are rare. There are many studies that implicated the association of GBS and arboviruses and point it to one of the main neurological manifestation of these infections. More research and consistent data are needed to clarify unanswered questions and guide public health measures.

Copyright © 2019. Published by Elsevier B.V.

KEYWORDS: Guillain-Barre syndrome; Zika virus; chikungunya; dengue fever

PMID: 31220435 DOI: 10.1016/j.actatropica.2019.105064

Keywords: Arbovirus; GBS; Chikungunya fever, Zika Virus, Dengue fever, Brazil.

——

#Systems analysis of #subjects acutely infected with the #Chikungunya virus (PLoS Pathog., abstract)

[Source: PLoS Pathogens, full page: (LINK). Abstract, edited.]

OPEN ACCESS /  PEER-REVIEWED / RESEARCH ARTICLE

Systems analysis of subjects acutely infected with the Chikungunya virus

Alessandra Soares-Schanoski, Natália Baptista Cruz, Luíza Antunes de Castro-Jorge, Renan Villanova Homem de Carvalho, Cliomar Alves dos Santos, Nancy da Rós, Úrsula Oliveira, Danuza Duarte Costa, Cecília Luíza Simões dos Santos, Marielton dos Passos Cunha, Maria Leonor Sarno Oliveira, Juliana Cardoso Alves, Regina Adalva de Lucena Couto Océa,  [ … ], Helder I. Nakaya

Published: June 18, 2019 / DOI: https://doi.org/10.1371/journal.ppat.1007880 / This is an uncorrected proof.

 

Abstract

The largest ever recorded epidemic of the Chikungunya virus (CHIKV) broke out in 2004 and affected four continents. Acute symptomatic infections are typically associated with the onset of fever and often debilitating polyarthralgia/polyarthritis. In this study, a systems biology approach was adopted to analyze the blood transcriptomes of adults acutely infected with the CHIKV. Gene signatures that were associated with viral RNA levels and the onset of symptoms were identified. Among these genes, the putative role of the Eukaryotic Initiation Factor (eIF) family genes and apolipoprotein B mRNA editing catalytic polypeptide-like (APOBEC3A) in the CHIKV replication process were displayed. We further compared these signatures with signatures induced by the Dengue virus infection and rheumatoid arthritis. Finally, we demonstrated that the CHIKV in vitro infection of murine bone marrow-derived macrophages induced IL-1 beta production in a mechanism that is significantly dependent on the inflammasome NLRP3 activation. The observations provided valuable insights into virus-host interactions during the acute phase and can be instrumental in the investigation of new and effective therapeutic interventions.

 

Author summary

The Chikungunya virus (CHIKV) has infected millions of people worldwide and presents a serious public health issue. Acute symptomatic infections caused by contracting this mosquito-transmitted arbovirus are typically associated with an abrupt onset of fever and often debilitating polyarthralgia/ polyarthritis, as well as prolonged periods of disability in some patients. These dramatic effects call for a careful evaluation of the molecular mechanisms involved in this puzzling infection. By analyzing the blood transcriptome of adults acutely infected with CHIKV, we were able to provide a detailed picture of the early molecular events induced by the infection. Additionally, the systems biology approach revealed genes that can be investigated extensively as probable therapeutic targets for the disease.

___

Citation: Soares-Schanoski A, Baptista Cruz N, de Castro-Jorge LA, de Carvalho RVH, Santos CAd, Rós Nd, et al. (2019) Systems analysis of subjects acutely infected with the Chikungunya virus. PLoS Pathog 15(6): e1007880. https://doi.org/10.1371/journal.ppat.1007880

Editor: David H. O’Connor, University of Wisconsin, UNITED STATES

Received: February 1, 2019; Accepted: May 30, 2019; Published: June 18, 2019

Copyright: © 2019 Soares-Schanoski et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All RNA-seq raw data is available at the NCBI in BioProject: PRJNA507472 and the BioSample Range from SAMN10847030 to SAMN10847088

Funding: H.I.N. is supported by the São Paulo Research Foundation (FAPESP; grants 2017/50137-3, 2012/19278-6, and 2013/08216-2). A.S.S. is supported by Butantan Foundation, CNPq (Grant 443371/2016-4) and Brazilian Health Ministry. R.A. is supported by FINEP Grant 0116005600. D.R.R. has a postdoctoral fellowship from CNPq. J.C.A. has a postdoctoral fellowship from CAPES – Finance Code 001. M.P.C. has a PhD fellowship from FAPESP – 2016/08204-2. I.J.A is supported by São Paulo Research Foundation (FAPESP; grant: CEPID 2013/07467-1). P.L.H is supported by Butantan Foundation, CNPq 306992/2014-0 and Fapesp 2015/25055-8. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Keywords: Chikungunya fever, Viral pathogenesis.

——