dengue fever – ETIDIoH

Covert cases of #SARS- CoV-2: An obscure but present #danger in regions endemic for #Dengue and #Chikungunya viruses (PLOS One, abstract)

[Source: PLOS One, full page: (LINK). Abstract, edited.]

OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE

Covert cases of Severe Acute Respiratory Syndrome Coronavirus 2: An obscure but present danger in regions endemic for Dengue and Chikungunya viruses

Lorenzzo Lyrio Stringari , Michel Norbim de Souza , Nésio Fernandes de Medeiros Junior, Jaqueline Pegoretti Goulart, Camila Giuberti, Reynaldo Dietze, Rodrigo Ribeiro-Rodrigues

Published: January 6, 2021 | DOI: https://doi.org/10.1371/journal.pone.0244937

Abstract

Background

The impact of SARS-CoV-2 in regions endemic for both Dengue and Chikungunya is still not fully understood. Considering that symptoms/clinical features displayed during Dengue, Chikungunya and SARS-CoV-2 acute infections are similar, undiagnosed cases of SARS-CoV-2 in co-endemic areas may be more prevalent than expected. This study was conducted to assess the prevalence of covert cases of SARS-CoV-2 among samples from patients with clinical symptoms compatible with either Dengue or Chikungunya viral infection in the state of Espírito Santo, Brazil.

Methods

Presence of immunoglobulin G (IgG) antibody specific to SARS-CoV-2 nucleoprotein was detected using a chemiluminescent microparticle immunoassay in samples from 7,370 patients, without previous history of COVID-19 diagnosis, suspected of having either Dengue (n = 1,700) or Chikungunya (n = 7,349) from December 1st, 2019 to June 30th, 2020.

Findings

Covert cases of SARS-CoV-2 were detected in 210 (2.85%) out of the 7,370 serum samples tested. The earliest undiagnosed missed case of COVID-19 dated back to a sample collected on December 18, 2019, also positive for Dengue Virus. Cross-reactivity with either Dengue virus or other common coronaviruses were not observed.

Interpretation

Our findings demonstrate that concomitant Dengue or Chikungunya outbreaks may difficult the diagnosis of SARS-CoV-2 infections. To our knowledge, this is the first study to demonstrate, with a robust sample size (n = 7,370) and using highly specific and sensitive chemiluminescent microparticle immunoassay method, that covert SARS-CoV-2 infections are more frequent than previously expected in Dengue and Chikungunya hyperendemic regions. Moreover, our results suggest that SAR-CoV-2 cases were occurring prior to February, 2020, and that these undiagnosed missed cases may have contributed to the fast expansion of SARS-CoV-2 outbreak in Brazil. Data presented here demonstrate that in arboviral endemic regions, SARS-CoV-2 infection must be always considered, regardless of the existence of a previous positive diagnosis for Dengue or Chikungunya.

Citation: Stringari LL, de Souza MN, de Medeiros Junior NF, Goulart JP, Giuberti C, Dietze R, et al. (2021) Covert cases of Severe Acute Respiratory Syndrome Coronavirus 2: An obscure but present danger in regions endemic for Dengue and Chikungunya viruses. PLoS ONE 16(1): e0244937. https://doi.org/10.1371/journal.pone.0244937

Editor: Francesco Di Gennaro, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, ITALY

Received: December 1, 2020; Accepted: December 20, 2020; Published: January 6, 2021

Copyright: © 2021 Stringari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the manuscript.

Funding: This work was supported by the Brazilian Ministry of Health and by the Espirito Santo State Government.

Competing interests: The authors have declared that no competing interests exist.

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Keywords: SARS-CoV-2; COVID-19; Serology; Dengue fever; Chikungunya; Brazil.

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Previous #dengue infection and #mortality in #COVID19 (Clin Infect Dis., abstract)

[Source: Clinical Infectious Diseases, full page: (LINK). Abstract, edited.]

Previous dengue infection and mortality in COVID-19

Odilson M Silvestre, MD, PhD, MPH, Letícia R Costa, Bianca V R Lopes, Mariana R Barbosa, Kárenn K P Botelho, Kelvyn L C Albuquerque, Anna G S Souza, Lorran A Coelho, Anderson J de Oliveira, Cínthia B Barantini, Sebastião A V M Neves, MD, PhD, Wilson Nadruz, MD, PhD, James H Maguire, MD, MPH, Miguel M Fernandes-Silva, MD, PhD, MPH

Clinical Infectious Diseases, ciaa1895, https://doi.org/10.1093/cid/ciaa1895

Published: 29 December 2020

Abstract

We studied 2351 participants with COVID-19, 1177 (50%) reported previous dengue infection. Those without previous dengue had a higher risk of death (hazard ratio: 0.44; 95% CI: 0.22 to 0.89; p = 0.023) in 60-day follow-up. These findings raise the possibility that dengue might induce immunological protection against SARS COV-2.

COVID-19, dengue, mortality

Issue Section: Brief Report

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

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Keywords: SARS-CoV-2; COVID-19; Dengue fever.

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Characteristics of Patients Co-infected with #SARS-CoV-2 and #Dengue Virus, Buenos Aires, #Argentina, March–June 2020 (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 27, Number 2—February 2021 | Synopsis

Characteristics of Patients Co-infected with Severe Acute Respiratory Syndrome Coronavirus 2 and Dengue Virus, Buenos Aires, Argentina, March–June 2020

Lucila M. Carosella, Daniel Pryluka, Aldo Maranzana, Laura Barcan, Rosana Cuini, Cristina Freuler, Alfredo Martinez, Tomás Rivero Equiza, Carolina Rodriguez Peria, Diego Yahni, Martin E. Stryjewski , and for the COVIDENGUE Study Group

Author affiliations: Centro de Educación Médica e Investigaciones Clínicas, Buenos Aires, Argentina (L.M. Carosella, A. Martinez, T. Rivero Equiza, M.E. Stryjewski); Sanatorio Otamendi, Buenos Aires (D. Pryluka); General Hospital de Agudos Parmenio Piñero Gobierno de la Ciudad, Buenos Aires (A. Maranzana); Hospital Italiano de Buenos Aires, Buenos Aires (L. Barcan); Hospital de Agudos Dr. Teodoro Álvarez, Buenos Aires (R. Cuini); Hospital Alemán, Buenos Aires (C. Freuler); Sanatorio de Los Arcos, Buenos Aires (C. Rodriguez Peria),; Sanatorio Mutual del Transporte Automotor, Buenos Aires (D. Yahni)

Abstract

An epidemic of dengue virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) co-infections occurred in Argentina during 2020. We describe the clinical characteristics and outcomes in a cohort of patients hospitalized because of co-infection. We retrospectively identified 13 patients from different hospitals in Buenos Aires who had confirmed infection with SARS-CoV-2 and dengue virus and obtained clinical and laboratory data from clinical records. All patients had febrile disease when hospitalized. Headache was a common symptom. A total of 8 patients had respiratory symptoms, 5 had pneumonia, and 3 had rash. Nearly all patients had lymphopenia when hospitalized. No patients were admitted to an intensive care unit or died during follow up. Co-infection with SARS-CoV-2 and dengue virus can occur in patients living in areas in which both viruses are epidemic. The outcome of these patients did not seem to be worse than those having either SARS-CoV-2 or dengue infection alone.

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Keywords: SARS-CoV-2; COVID-19; Dengue fever; Argentina.

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A Case Study of Dual Infection of #Dengue and #COVID19: Presenting as #Multiorgan #Dysfunction in an #Infant (J Trop Pediatr., abstract)

[Source: Journal of Tropical Pediatrics, full page: (LINK). Abstract, edited.]

A Case Study of Dual Infection of Dengue and COVID-19: Presenting as Multiorgan Dysfunction in an Infant

Maha Ashraf Kazi, MBBS, Sanajit Ghosh, MBBS, Satyabrata Roychowdhury, MD, Prabhas Prasun Giri, MD, Mihir Sarkar, MD

Journal of Tropical Pediatrics, fmaa080, https://doi.org/10.1093/tropej/fmaa080

Published: 06 December 2020

Abstract

Dengue is a major health concern in South Asian countries transmitted by bite of day breeder mosquitoes Aedes aegypti and Aedes albopictus. Severity of plasma leak, shock, bleeding tendency and other organ dysfunction can be more pronounced in infants. The management becomes further complicated in the presence of a co-existing COVID-19 infection. Although COVID-19 infection is usually asymptomatic or has mild manifestations in children, however in presence of serious co-infection like dengue it can modify the course of the illness and lead to drastic consequences. Here, we present one such case of a 9-month-old female child who tested positive for dengue as well as COVID-19 during the ongoing corona pandemic and went on to develop shock, encephalopathy with deranged liver enzymes but managed to overcome all odds and recover from the disease by day 14 of illness.

severe dengue, COVID-19, co-infection, infant

Issue Section: Case Report

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Keywords: SARS-CoV-2; COVID-19; Dengue fever; Pediatrics.

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A Versatile #Reporter #System to #Monitor Virus Infected Cells and Its Application to #Dengue Virus and #SARS-CoV-2 (J Virol., abstract)

[Source: Journal of Virology, full page: (LINK). Abstract, edited.]

A Versatile Reporter System to Monitor Virus Infected Cells and Its Application to Dengue Virus and SARS-CoV-2

Felix Pahmeier, Christopher J. Neufeldt, Berati Cerikan, Vibhu Prasad, Costantin Pape, Vibor Laketa, Alessia Ruggieri, Ralf Bartenschlager, Mirko Cortese

DOI: 10.1128/JVI.01715-20

ABSTRACT

Positive-strand RNA viruses have been the etiological agents in several major disease outbreaks over the last few decades. Examples of this include flaviviruses, such as dengue virus and Zika virus that cause millions of yearly infections around the globe, and coronaviruses, such as SARS-CoV-2, the source of the current pandemic. The severity of outbreaks caused by these viruses stresses the importance of research aimed at determining methods to limit virus spread and to curb disease severity. Such studies require molecular tools to decipher virus-host interactions and to develop effective treatments. Here, we describe the generation and characterization of a reporter system that can be used to visualize and identify cells infected with dengue virus or SARS-CoV-2. This system is based on viral protease activity that mediates cleavage and nuclear translocation of an engineered fluorescent protein stably expressed in cells. We show the suitability of this system for live cell imaging, for visualization of single infected cells, and for screening and testing of antiviral compounds. With the integrated modular building blocks, this system is easy to manipulate and can be adapted to any virus encoding a protease, thus offering a high degree of flexibility.

IMPORTANCE

Reporter systems are useful tools for fast and quantitative visualization of virus infected cells within a host cell population. Here we describe a reporter system that takes advantage of virus-encoded proteases that are expressed in infected cells to cleave an ER-anchored fluorescent protein fused to a nuclear localization sequence. Upon cleavage, the GFP moiety translocates to the nucleus, allowing for rapid detection of the infected cells. Using this system, we demonstrate reliable reporting activity for two major human pathogens from the Flaviviridae and the Coronaviridae families: dengue virus and SARS-CoV-2. We apply this reporter system to live cell imaging and use it for proof-of-concept to validate antiviral activity of a nucleoside analogue. This reporter system is not only an invaluable tool for the characterization of viral replication, but also for the discovery and development of antivirals that are urgently needed to halt the spread of these viruses.

This article is made available via the PMC Open Access Subset for unrestricted noncommercial re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Keywords: Flavivirus; Dengue fever; SARS-CoV-2; Virology.

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#COVID19 #lockdown: #Impact #assessment on #Aedes larval indices, #breeding habitats, effects on #vector control programme and prevention of #dengue outbreaks (Heliyon, abstract)

[Source: Heliyon, full page: (LINK). Abstract, edited.]

COVID-19 lockdown: Impact assessment on Aedes larval indices, breeding habitats, effects on vector control programme and prevention of dengue outbreaks

Appadurai Daniel Reegan, Munusamy Rajiv Gandhi, Antony Cruz Asharaja, Chitra Devi, Shanmugam Perumal Shanthakumar

Open Access | Published: October 05, 2020 | DOI: https://doi.org/10.1016/j.heliyon.2020.e05181

ABSTRACT

Aedes aegypti (Linn.) and Aedes albopictus (Skuse) are widespread vector mosquitoes responsible for the transmission of various disease-causing viruses to human including dengue virus (DENV). India is endemic for dengue disease and both of these vector mosquitoes are well established throughout India. Since, Aedes mosquitoes breeds in containers, WHO recommends to do a regular immature surveillance and implement appropriate control measures. Owing to the current COVID-19 pandemic, most of the countries have implemented continuous shutdown/lockdown, which affected the routine Aedes surveillance and vector control measures. In India, the first nation-wide lockdown was implemented on 24th, March 2020. As of now, Government of India has extended the lockdown till 30th, June 2020. In the present study, two rounds of Aedes surveillance was carried out in two localities of Bangalore City (urban) of Karnataka State, India during the COVID-19 lockdown days and results were compared with pre- lockdown surveillance data to assess the impact of lockdown on Aedes larval indices, breeding habitats and dengue vector control programme. The recorded house index (HI) and Breteau index (BI) were 6.6 and 9.3 in K.P. Agrahara and 4.0 and 5.3 in Palace Guttahalli during pre-lockdown survey. The house index (HI) and Breteau index (BI) were found to be increased to 26.6 and 34.6 in K.P. Agrahara and 21.3 and 28.0 in Palace Guttahalli during the COVID-19 lockdown second survey. Aedes immature density has drastically increased in both the localities due to temporarily discontinued Aedes surveillance, larval control activities like source reduction and anti-larval measures during COVID-19 lockdown. The high indices show that the dengue virus transmission will be increasing in the coming days. The results highly recommend to implement the Aedes vector control programme with limited health staffs following the physical distance and other protectives measures to prevent dengue outbreaks.

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Keywords: SARS-CoV-2; COVID-19; Dengue Fever; Mosquitoes; Aedes spp.; India; Quarantine.

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First #autochthonous #dengue #outbreak in #Italy, August 2020 (Euro Surveill., abstract)

[Source: Eurosurveillance, full page: (LINK). Abstract, edited.]

First autochthonous dengue outbreak in Italy, August 2020

Luca Lazzarini1 , Luisa Barzon2,3,4 , Felice Foglia5 , Vinicio Manfrin1 , Monia Pacenti4 , Giacomina Pavan6 , Mario Rassu6 , Gioia Capelli2,7 , Fabrizio Montarsi2,7 , Simone Martini2,8 , Francesca Zanella2,9 , Maria Teresa Padovan5 , Francesca Russo2,9 , Federico Gobbi2,10

Affiliations: 1 Department of Infectious and Tropical Diseases, San Bortolo Hospital, Vicenza, Italy; 2 Veneto Region Arbovirosis Task Force, Venezia, Italy; 3 Department of Molecular Medicine, University of Padova, Italy; 4 Microbiology and Virology Unit, Padova University Hospital, Padova, Italy; 5 Department of Public Health AULSS8, Vicenza, Italy; 6 Department of Microbiology, San Bortolo Hospital, Vicenza, Italy; 7 Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Padova, Italy; 8 Entostudio s.r.l., Ponte San Nicolò, Padova, Italy; 9 Direzione Prevenzione, Sicurezza Alimentare Veterinaria – Regione Veneto, Venice, Italy; 10 Department of Infectious/Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy

Correspondence: Luca Lazzarini

Citation style for this article: Lazzarini Luca , Barzon Luisa , Foglia Felice , Manfrin Vinicio , Pacenti Monia , Pavan Giacomina , Rassu Mario , Capelli Gioia , Montarsi Fabrizio , Martini Simone , Zanella Francesca , Padovan Maria Teresa , Russo Francesca , Gobbi Federico . First autochthonous dengue outbreak in Italy, August 2020. Euro Surveill. 2020;25(36):pii=20-01606. https://doi.org/10.2807/1560-7917.ES.2020.25.36.2001606

Received: 31 Aug 2020; Accepted: 10 Sep 2020

Abstract

In August 2020, during the coronavirus disease (COVID-19) pandemic, five locally acquired cases of dengue virus type 1 were detected in a family cluster in Vicenza Province, North-East Italy where Aedes albopictus mosquitoes are endemic. The primary case was an importation from West Sumatra, Indonesia. This is the first outbreak of autochthonous dengue reported in Italy. During the COVID-19 pandemic, screening of febrile travelers from endemic countries is crucial in areas where competent vectors are present.

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Keywords: Dengue fever; Aedes albopictus; Mosquitoes; Italy.

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#COVID19 and #dengue #coinfection in a returning #traveller (J Trav Med., abstract)

[Source: Journal of Travel Medicine, full page: (LINK). Abstract, edited.]

COVID-19 and dengue co-infection in a returning traveller

Loïc Epelboin, MD, PhD, Renaud Blondé, MD, Mathieu Nacher, MD, PhD, Patrice Combe, MD, Louis Collet, MD

Journal of Travel Medicine, taaa114, https://doi.org/10.1093/jtm/taaa114

Published: 13 July 2020

Abstract

In march 2020, a pandemic of respiratory infection due to the SARS-CoV2 is underway, dengue fever epidemics are at the same time present in many regions of the inter-equatorial zone. We report the first cases of covid19-dengue co-infection, which occurred in Mayotte, a French island in the Indian Ocean.

coronavirus, COVID-19, severe acute respiratory syndrome coronavirus 2, pandemic; dengue fever, tropical diseases, France, Western Europe, Mayotte; coinfection

Issue Section: RAPID COMMUNICATION

This content is only available as a PDF.

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Keywords: SARS-CoV-2; COVID-19; Dengue fever; Mayotte.

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#Coinfection, co‐epidemics of #Covid19 and #Dengue in dengue endemic countries: a serious health concern (J Med Virol., abstract)

[Source: Journal of Medical Virology, full page: (LINK). Abstract, edited.]

Co‐infection, co‐epidemics of Covid‐19 and Dengue in dengue endemic countries: a serious health concern

Md. Asaduzzaman Miah, Asmaul Husna

First published: 07 July 2020 | DOI: https://doi.org/10.1002/jmv.26269

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/jmv.26269

Abstract

During the Covid‐19 pandemic, dengue cases have been increased in most of the dengue‐endemic countries in the world. Therefore, in these countries, both Covid‐19 and Dengue are occurring simultaneously. The dengue‐endemic countries are at the risk of possible co‐infection and co‐epidemics in where Covid‐19 and dengue disease are co‐existing, and the viruses are cocirculating. Thus, co‐infection and co‐epidemics trigger alert, especially for the health care professionals in the dengue‐endemic countries.

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Keywords: SARS-CoV-2; COVID-19; Dengue fever.

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#Dengue and #COVID19, overlapping #epidemics? An Analysis from #Colombia (J Med Virol., abstract)

[Source: Journal of Medical Virology, full page: (LINK). Abstract, edited.]

Dengue and COVID‐19, overlapping epidemics? An Analysis from Colombia

Jaime A. Cardona‐Ospina, Kovy Arteaga‐Livias, Wilmer E. Villamil‐Gómez, Carlos E. Pérez‐Díaz, D. Katterine Bonilla‐Aldana, Álvaro Mondragon‐Cardona, Marco Solarte, Ernesto Martinez, Jose Millan‐Oñate, Eduardo López, Pio López, Juan‐Carlos Navarro, Luis Perez‐Garcia, Euler Mogollon‐Rodriguez, Alfonso J. Rodríguez‐Morales, Alberto Paniz‐Mondolfi, …

First published: 19 June 2020 | DOI: https://doi.org/10.1002/jmv.26194

Abstract

Coronavirus Disease 2019 (COVID‐19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2), has rapidly spread throughout Latin America, a region swept by multiple previous and ongoing epidemics. There are significant concerns that the arrival of COVID‐19 is currently overlapping with other viruses, particularly dengue, in various endo‐epidemic regions across South America. In this report, we analyzed trends for both viral infections in Colombia during the first 20 epidemiological weeks of 2020. From January 1st to May 16th, 2020 (epidemiological weeks, EW, 1‐20), a total of 52,679 cases of dengue and 14,943 cases of COVID‐19 have been confirmed in Colombia. As both conditions may potentially lead to fatal outcomes, especially in patients with chronic co‐morbidities, overlapping infections and co‐occurrence may increase the number of patients requiring intensive care and mechanical ventilation. In regions such as Valle del Cauca, intensified preparation for such scenarios should be pondered, and further studies should be performed to address this critical issue in a timely matter.

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Keywords: SARS-CoV-2; COVID-19; Dengue fever; Colombia.

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