[Source: Cell Press, full page: (LINK). Abstract, edited.]
Journal pre-proof | DOI: 10.1016/j.chom.2020.03.023
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© 2020 The Author(s).
Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
Young-Il Kim1,2, Seong-Gyu Kim1, Se-Mi Kim1, Eun-Ha Kim1,2, Su-Jin Park1,2 2 , Kwang-Min Yu1,2, Jae-Hyung Chang1, Eun Ji Kim1, Seunghun Lee1, Mark Anthony B. Casel1,2, Jiyee Um4 3 ,Min-Suk Song1,2, Hye Won Jeong1, Van Dam Lai3, Yeonjae Kim4, Bum Sik Chin4 4 , Jun-Sun Park4, Ki-Hyun Chung4, Suan-Sin Foo5, Haryung Poo6, In-pil Mo3, OK Jun Lee1 5 , Richard J Webby7, Jae U. Jung5,*, and Young Ki Choi1,2,8*
1 College of Medicine and Medical Research Institute, Chungbuk National University,
9 Cheongju, Republic of Korea; 2 Zoonotic Infectious Diseases Research Center, Chungbuk National University, Cheongju, Korea; 3College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea; 4 Research institute of Public Health, National Medical Center, Seoul, Republic of Korea; 5 Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; 6Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Daejeon, Republic of Korea; 7 Division of Virology, Department of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA; 8 Lead Contact
*Correspondence: firstname.lastname@example.org (J. J), email@example.com (Y.K.C) *Jae U. Jung – Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China and rapidly spread worldwide. To prevent SARS-CoV-2 dissemination, understanding the in vivo characteristics of SARS-CoV 2 is a high priority. We report a ferret model of SARS-CoV-2 infection and transmission that recapitulates aspects of human disease. SARS-CoV-2-infected ferrets exhibit elevated body temperatures and virus replication. Although fatalities were not observed, SARS-CoV-2- infected ferrets shed virus in nasal washes, saliva, urine and feces up to 8 days post infection. At 2 days post-contact, SARS-CoV-2 was detected in all naïve direct contact ferrets. Furthermore, a few naïve indirect contact ferrets were positive for viral RNA, suggesting airborne transmission. Viral antigens were detected in nasal turbinate, trachea, lungs, and intestine with acute bronchiolitis present in infected lungs. Thus, ferrets represent an infection and transmission animal model of COVID-19 that may facilitate development of SARS-CoV-2 therapeutics and vaccines.
Keywords: 2019-novel coronavirus (2019-nCoV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), novel coronavirus disease (COVID-19), virus shedding, transmission, ferrets
Keywords: SARS-CoV-2; COVID-19; Animal models.