[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]
SARS-CoV-2-specific antibody detection for sero-epidemiology: a multiplex analysis approach accounting for accurate seroprevalence
Gerco den Hartog, Rutger M Schepp, Marjan Kuijer, Corine GeurtsvanKessel, Josine van Beek, Nynke Rots, Marion P G Koopmans, Fiona R M van der Klis, Robert S van Binnendijk
The Journal of Infectious Diseases, jiaa479, https://doi.org/10.1093/infdis/jiaa479
Published: 08 August 2020
The COVID-19 pandemic necessitates a better understanding of the kinetics of antibody production induced by infection with SARS-CoV-2. We aimed to develop a high throughput multiplex assay to detect antibodies to SARS-CoV-2 to assess immunity to the virus in the general population.
Spike protein subunits S1 and RBD, and Nucleoprotein were coupled to distinct microspheres. Sera collected before the emergence of SARS-CoV-2 (N=224), and of non-SARS-CoV-2 influenza-like illness (N=184), and laboratory-confirmed cases of SARS-CoV-2 infection (N=115) with various severity of COVID-19 were tested for SARS-CoV-2-specific concentrations of IgG.
Our assay discriminated SARS-CoV-2-induced antibodies and those induced by other viruses. The assay obtained a specificity between 95.1 and 99.0% with a sensitivity ranging from 83.6-95.7%. By merging the test results for all 3 antigens a specificity of 100% was achieved with a sensitivity of at least 90%. Hospitalized COVID-19 patients developed higher IgG concentrations and the rate of IgG production increased faster compared to non-hospitalized cases.
The bead-based serological assay for quantitation of SARS-CoV-2-specific antibodies proved to be robust and can be conducted in many laboratories. Finally, we demonstrated that testing of antibodies against different antigens increases sensitivity and specificity compared to single antigen-specific IgG determination.
COVID-19, IgG, Spike S1, RBD, Nucleoprotein, endemic coronavirus, multiplex bead-based immune assay, specificity, sensitivity, Influenza-like Illness (ILI)
Topic: epidemiology – coronavirus – antibody formation – antigens – immunity – laboratory – microspheres – nucleoproteins – protein subunits – serologic tests – immunoglobulin g – infections – antibodies – kinetics – viruses – severe acute respiratory syndrome – serum – flu-like illness – laboratory test finding – seroprevalence – sars-cov-2 –
covid-19 – coronavirus pandemic
Issue Section: Major Article
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© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.
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Keywords: SARS-CoV-2; COVID-19; Serology; Seroprevalence.