Complete #Genome #Sequence of a #Colombian #Zika Virus Strain Obtained from BALB/c Mouse #Brain after Intraperitoneal Inoculation (Microbiol Resourc Announc., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Microbiol Resour Announc. 2019 Nov 14;8(46). pii: e01719-18. doi: 10.1128/MRA.01719-18.

Complete Genome Sequence of a Colombian Zika Virus Strain Obtained from BALB/c Mouse Brain after Intraperitoneal Inoculation.

Laiton-Donato K1, Álvarez-Díaz DA2, Rengifo AC3, Torres-Fernández O2, Usme-Ciro JA1,4, Rivera JA2, Santamaría G2, Naizaque J2, Monroy-Gómez J2,5, Sarmiento L2, Gunturiz ML6, Muñoz A7, Vanegas R7, Rico A1, Pardo L1, Peláez-Carvajal D1.

Author information: 1 Grupo de Virología, Dirección de Redes en Salud Pública, Instituto Nacional de Salud, Bogotá, DC, Colombia. 2 Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, DC, Colombia. 3 Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, DC, Colombia 4 Centro de Investigación en Salud para el Trópico-CIST, Universidad Cooperativa de Colombia, Santa Marta, Colombia. 5 Escuela Colombiana de Rehabilitación, Bogotá, DC, Colombia. 6 Equipo Banco de Proyectos, Dirección de Investigación en Salud Pública, Bogotá, DC, Colombia. 7 Grupo Animales de Laboratorio, Dirección de Producción, Instituto Nacional de Salud, Bogotá, DC, Colombia.



A Zika virus (ZIKV) strain was isolated from an acute febrile patient during the Zika epidemics in Colombia. The strain was intraperitoneally inoculated into BALB/c mice, and 7 days postinoculation, neurological manifestations and ZIKV infection in the brain were demonstrated. The reported genome sequence is highly related to strains circulating in the Americas.

Copyright © 2019 Laiton-Donato et al.

PMID: 31727724 DOI: 10.1128/MRA.01719-18

Keywords: Zika Virus; Colombia.


#Cost-effectiveness of Prophylactic #Zika Virus #Vaccine in the #Americas (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 25, Number 12—December 2019 / Research

Cost-effectiveness of Prophylactic Zika Virus Vaccine in the Americas

Affan Shoukat, Thomas Vilches, and Seyed M. Moghadas

Author affiliations: Author affiliations: York University, Toronto, Ontario, Canada (A. Shoukat, S.M. Moghadas); São Paulo State University, Botucatu SP, Brazil (T. Vilches)



Zika virus remains a major public health concern because of its association with microcephaly and other neurologic disorders in newborns. A prophylactic vaccine has the potential to reduce disease incidence and eliminate birth defects resulting from prenatal Zika virus infection in future outbreaks. We evaluated the cost-effectiveness of a Zika vaccine candidate, assuming a protection efficacy of 60%–90%, for 18 countries in the Americas affected by the 2015–2017 Zika virus outbreaks. Encapsulating the demographics of these countries in an agent-based model, our results show that vaccinating women of reproductive age would be very cost-effective for sufficiently low (<$16) vaccination costs per recipient, depending on the country-specific Zika attack rate. In all countries studied, the median reduction of microcephaly was >75% with vaccination. These findings indicate that targeted vaccination of women of reproductive age is a noteworthy preventive measure for mitigating the effects of Zika virus infection in future outbreaks.

Keywords: Zika Virus; Vaccines.


#Subclinical in #utero #Zika virus #infection is associated with #interferon alpha #sequelae and sex-specific molecular #brain #pathology in asymptomatic porcine offspring (PLoS Pathog., abstract)

[Source: PLoS Pathogens, full page: (LINK). Abstract, edited.]


Subclinical in utero Zika virus infection is associated with interferon alpha sequelae and sex-specific molecular brain pathology in asymptomatic porcine offspring

Ivan Trus , Daniel Udenze, Brian Cox , Nathalie Berube, Rebecca E. Nordquist, Franz Josef van der Staay, Yanyun Huang, Gary Kobinger, David Safronetz, Volker Gerdts, Uladzimir Karniychuk


Published: November 14, 2019 / DOI:



Zika virus (ZIKV) infection during human pregnancy may lead to severe fetal pathology and debilitating impairments in offspring. However, the majority of infections are subclinical and not associated with evident birth defects. Potentially detrimental life-long health outcomes in asymptomatic offspring evoke high concerns. Thus, animal models addressing sequelae in offspring may provide valuable information. To induce subclinical infection, we inoculated selected porcine fetuses at the mid-stage of development. Inoculation resulted in trans-fetal virus spread and persistent infection in the placenta and fetal membranes for two months. Offspring did not show congenital Zika syndrome (e.g., microcephaly, brain calcifications, congenital clubfoot, arthrogryposis, seizures) or other visible birth defects. However, a month after birth, a portion of offspring exhibited excessive interferon alpha (IFN-α) levels in blood plasma in a regular environment. Most affected offspring also showed dramatic IFN-α shutdown during social stress providing the first evidence for the cumulative impact of prenatal ZIKV exposure and postnatal environmental insult. Other eleven cytokines tested before and after stress were not altered suggesting the specific IFN-α pathology. While brains from offspring did not have histopathology, lesions, and ZIKV, the whole genome expression analysis of the prefrontal cortex revealed profound sex-specific transcriptional changes that most probably was the result of subclinical in utero infection. RNA-seq analysis in the placenta persistently infected with ZIKV provided independent support for the sex-specific pattern of in utero-acquired transcriptional responses. Collectively, our results provide strong evidence that two hallmarks of fetal ZIKV infection, altered type I IFN response and molecular brain pathology can persist after birth in offspring in the absence of congenital Zika syndrome.


Author summary

A number of studies showed that Zika virus (ZIKV) can cause severe abnormalities in fetuses, e.g., brain lesions, and subsequently life-long developmental and cognitive impairment in children. However, the majority of infections in pregnant women are subclinical and are not associated with developmental abnormalities in fetuses and newborns. It is known that disruptions to the in utero environment during fetal development can program increased risks for disease in adulthood. For this reason, children affected in utero even by mild ZIKV infection can appear deceptively healthy at birth but develop immune dysfunction and brain abnormalities during postnatal development. Here, we used the porcine model of subclinical fetal ZIKV infection to determine health sequelae in offspring which did not show apparent signs of the disease. We demonstrated that subclinical fetal infection was associated with abnormal immunological responses in apparently healthy offspring under normal environmental conditions and during social stress. We also showed silent sex-specific brain pathology as represented by altered gene expression. Our study provides new insights into potential outcomes of subclinical in utero ZIKV infection. It also emphasizes that further attempts to better understand silent pathology and develop alleviative interventions in ZIKV-affected offspring should take into account interactions of host factors, like sex, and environmental insults, like social stress.


Citation: Trus I, Udenze D, Cox B, Berube N, Nordquist RE, van der Staay FJ, et al. (2019) Subclinical in utero Zika virus infection is associated with interferon alpha sequelae and sex-specific molecular brain pathology in asymptomatic porcine offspring. PLoS Pathog 15(11): e1008038.

Editor: Ted C. Pierson, NIH, UNITED STATES

Received: May 8, 2019; Accepted: August 21, 2019; Published: November 14, 2019

Copyright: © 2019 Trus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the manuscript and its Supporting Information files.

Funding: Financial support was provided by Genome Canada, Emerging Issue Program grant #418402, the Public Health Agency of Canada and the Government of Saskatchewan through Innovation Saskatchewan #418836. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Keywords: Zika Virus; Pregnancy; Zika Congenital Infection; Zika Congenital Syndrome; Animal models.


#Zika virus #infection: A correlation between #prenatal ultrasonographic and #postmortem #neuropathologic changes (Neuropathology, abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Neuropathology. 2019 Nov 11. doi: 10.1111/neup.12603. [Epub ahead of print]

Zika virus infection: A correlation between prenatal ultrasonographic and postmortem neuropathologic changes.

Gutiérrez Sánchez LA1,2, Sandoval Martínez DK1,3, Díaz-Martínez LA1, Becerra Mojica CH1,2.

Author information: 1 School of Medicine, Health Faculty, Universidad Industrial de Santander, Bucaramanga, Colombia. 2 Department of Gynecology and Obstetrics, Hospital Universitario de Santander, Bucaramanga, Colombia. 3 Department of Pathology, Hospital Universitario de Santander, Bucaramanga, Colombia.



This study presents a correlation between prenatal ultrasonographic images and neuropathologic findings of postmortem tissue samples from five confirmed cases of perinatal Zika virus (ZIKV) infection belonging to the cohort of the ZEN Initiative in Bucaramanga, Colombia. Deaths occurred between June 2016 and March 2017. Mothers consulted with ZIKV infection clinical manifestations or fetal central nervous system (CNS) abnormalities or both. A detailed ultrasound scan and neurosonographic protocol was performed by maternal fetal specialists. Perinatal autopsies were performed following the Colombian National Health Institute’s ZIKV protocol. The autopsies were from two fetal deaths, and three early neonatal deaths. Gestational age was between 262/7 and 382/7 weeks. Two cases were classified as mild microcephaly. Few findings by ultrasound and pathology were found in case 1 because it was a late infection; the other cases presented findings corresponding to congenital Zika syndrome: craniofacial malformations, cerebellar hypoplasia, anomalies of the corpus callosum and ventriculomegaly, all confirmed in autopsy specimens. By ultrasonography, hyperechogenicities were seen in several brain structures, which correspond to cortical and periventricular calcifications, subependymal glial reactivity and perivascular rings. The ultrasound and pathological findings show a wide spectrum of CNS anomalies that confirm the neurotropic effect of the ZIKV, recognizing the neuroimaging findings of this disease (unilateral ventriculomegaly, alterations in the corpus callosum and cerebellum, and calcifications) are highly suggestive of ZIKV infection.

© 2019 Japanese Society of Neuropathology.

KEYWORDS: Zika virus infection; arthrogryposis; corpus callosum dysgenesis; lissencephaly; microcephaly

PMID: 31710135 DOI: 10.1111/neup.12603

Keywords: Zika Virus; Neuroimaging; Neuroinvasion; Pediatrics; Radiology.


Development of a Potent and Protective #Germline-Like #Antibody Lineage Against #Zika Virus in a #Convalescent #Human (Front Immunol., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Front Immunol. 2019 Oct 24;10:2424. doi: 10.3389/fimmu.2019.02424. eCollection 2019.

Development of a Potent and Protective Germline-Like Antibody Lineage Against Zika Virus in a Convalescent Human.

Gao F1, Lin X2, He L2, Wang R1, Wang H1, Shi X1, Zhang F3, Yin C3, Zhang L1, Zhu J2, Yu L3.

Author information: 1 Department of Basic Medical Sciences, Comprehensive AIDS Research Center, Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing, China. 2 Department of Integrative Structural and Computational Biology, Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, United States. 3 Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou, China.



Zika virus (ZIKV) specific neutralizing antibodies hold great promise for antibody-based interventions and vaccine design against ZIKV infection. However, their development in infected patients remains unclear. Here, we applied next-generation sequencing (NGS) to probe the dynamic development of a potent and protective ZIKV E DIII-specific antibody ZK2B10 isolated from a ZIKV convalescent individual. The unbiased repertoire analysis showed dramatic changes in the usage of antibody variable region germline genes. However, lineage tracing of ZK2B10 revealed limited somatic hypermutation and transient expansion during the 12 months following the onset of symptoms. The NGS-derived, germline-like ZK2B10 somatic variants neutralized ZIKV potently and protected mice from lethal challenge of ZIKV without detectable cross-reactivity with Dengue virus (DENV). Site-directed mutagenesis identified two residues within the λ chain, N31 and S91, that are essential to the functional maturation of ZK2B10. The repertoire and lineage features unveiled here will help elucidate the developmental process and protective potential of E DIII-directed antibodies against ZIKV infection.

Copyright © 2019 Gao, Lin, He, Wang, Wang, Shi, Zhang, Yin, Zhang, Zhu and Yu.

KEYWORDS: Guillain–Barré syndrome; Zika virus infection; antibody repertoire; microcephaly; neutralizing antibody; next-generation sequencing

PMID: 31708914 PMCID: PMC6821881 DOI: 10.3389/fimmu.2019.02424

Keywords: Zika Virus; Neutralizing antibodies.


#Maternal #Zika Virus #Infection: Association With Small-for-Gestational-Age #Neonates and Preterm #Birth (Obstet Gynecol., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Obstet Gynecol. 2019 Nov 4. doi: 10.1097/AOG.0000000000003577. [Epub ahead of print]

Maternal Zika Virus Infection: Association With Small-for-Gestational-Age Neonates and Preterm Birth.

Cooper HJ1, Iwamoto M, Lash M, Conners EE, Paladini M, Slavinski S, Fine AD, Kennedy J, Heinke D, Ciaranello A, Seage GR 3rd, Lee EH.

Author information: 1 Bureau of Communicable Disease, New York City Department of Health and Mental Hygiene, Queens, and the Bureau of Vital Statistics, New York City Department of Health and Mental Hygiene, New York, New York; and the Harvard T. H. Chan School of Public Health and the Medical Practice Evaluation Center, Division of Infectious Disease, Massachusetts General Hospital, Boston, Massachusetts.




To evaluate whether antenatal Zika virus infection is associated with risk of having a small-for-gestational-age (SGA) neonate, risk of preterm birth, and lower mean birth weight of term neonates.


For this retrospective observational study, we linked birth record data for women who delivered liveborn singleton neonates in New York City in 2016 to data for pregnant women with Zika virus infection reported to the New York City Health Department. We restricted the analysis to nonsmoking, nonwhite women and adjusted for maternal characteristics. Among women with antenatal Zika virus infection, we used modified Poisson regression to assess risks of having an SGA neonate and of delivering preterm, and linear regression to assess the association of infection with mean birth weight of term neonates.


Of 116,034 deliveries of singleton neonates in New York City in 2016, 251 (0.2%) were to women with antenatal Zika virus infection. A higher percentage of women with Zika virus infection delivered an SGA neonate compared with those without (11.2% vs 5.8%; adjusted relative risk [RR] 1.8; 95% CI 1.3-2.6). There was no difference in preterm birth prevalence for women with and without Zika virus infection (adjusted RR 1.0; 95% CI 0.69-1.6). Mean birth weight of term neonates born to women with Zika virus infection was 47 g less (95% CI -105 to 11 g); this difference was not statistically significant in crude or adjusted analyses.


For a cohort of New York City women, antenatal Zika virus infection was associated with an increased risk of having an SGA neonate, but not preterm birth or lower mean birth weight of term neonates. This supports a putative association between Zika virus infection during pregnancy and SGA.

PMID: 31698388 DOI: 10.1097/AOG.0000000000003577

Keywords: Zika Virus; Zika Congenital Infection; Pregnancy; USA.


#Vector-borne #transmission of #Zika virus in #Europe, southern #France, August 2019 (Euro Surveill., abstract)

[Source: Eurosurveillance, full page: (LINK). Abstract, edited.]

Vector-borne transmission of Zika virus in Europe, southern France, August 2019

Sandra Giron1, Florian Franke1, Anne Decoppet2, Bernard Cadiou3, Thierry Travaglini3, Laurence Thirion4, Guillaume Durand4,5, Charles Jeannin3, Grégory L’Ambert3, Gilda Grard4,5, Harold Noël6, Nelly Fournet6, Michelle Auzet-Caillaud2, Christine Zandotti5, Samer Aboukaïs2, Pascal Chaud1, Saby Guedj7, Lakri Hamouda7, Xavier Naudot8, Anne Ovize8, Clément Lazarus9, Henriette de Valk6, Marie-Claire Paty6, Isabelle Leparc-Goffart4,5

Affiliations: 1 Santé publique France (French National Public Health Agency), Marseille, France; 2 Regional Health Agency of Provence-Alpes-Côtes d’Azur (ARS Paca), Marseille, France; 3 Entente interdépartementale pour la démoustication du littoral méditerranéen (EID Méditerranée), Montpellier, France; 4 Unité des Virus Emergents (UVE: Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée Infection), Marseille, France; 5 Institut de Recherche Biomédicale des Armées, National Reference Laboratory for Arboviruses, Marseille, France; 6 Santé publique France (French National Public Health Agency), Saint-Maurice, France; 7 Médecin généraliste, Hyères, France; 8 Eurofins Biomnis, Lyon, France; 9 Public Health Emergency Operations Centre, Division of Surveillance and Health Security, Ministry of Health, General Directorate for Health, Health Emergencies Crisis Management Centre, Paris, France

Correspondence:  Harold Noel

Citation style for this article: Giron Sandra, Franke Florian, Decoppet Anne, Cadiou Bernard, Travaglini Thierry, Thirion Laurence, Durand Guillaume, Jeannin Charles, L’Ambert Grégory, Grard Gilda, Noël Harold, Fournet Nelly, Auzet-Caillaud Michelle, Zandotti Christine, Aboukaïs Samer, Chaud Pascal, Guedj Saby, Hamouda Lakri, Naudot Xavier, Ovize Anne, Lazarus Clément, de Valk Henriette, Paty Marie-Claire, Leparc-Goffart Isabelle. Vector-borne transmission of Zika virus in Europe, southern France, August 2019. Euro Surveill. 2019;24(45):pii=1900655.

Received: 29 Oct 2019;   Accepted: 07 Nov 2019



On 1 October 2019, a locally-acquired Zika virus disease case was laboratory confirmed in Hyères, Var department. Active case finding identified two additional locally-acquired cases living within 90 m, with symptom onset 8 days before the index case. Extensive patient interviews did not yield information supporting transmission through sexual contact or substances of human origin. Vector-borne transmission by local Aedes albopictus mosquitoes is the most likely mode of transmission. Here we describe the public health response.

©  This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords: Zika Virus; France.