[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Vet Immunol Immunopathol. 2019 Sep 9;218:109939. doi: 10.1016/j.vetimm.2019.109939. [Epub ahead of print]
Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein.
Haverkamp AK1, Bosch BJ2, Spitzbarth I3, Lehmbecker A3, Te N4, Bensaid A4, Segalés J5, Baumgärtner W6.
Author information: 1 Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany. 2 Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, the Netherlands. 3 Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany; Center for Systems Neuroscience, 30559 Hannover, Germany. 4 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. 5 Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Barcelona, Spain; UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. 6 Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany; Center for Systems Neuroscience, 30559 Hannover, Germany. Electronic address: Wolfgang.Baumgaertner@tiho-hannover.de.
Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.
Copyright © 2019 Elsevier B.V. All rights reserved.
KEYWORDS: Immunohistochemistry; Middle East respiratory syndrome coronavirus; Monoclonal human antibodies; Spike protein
PMID: 31526954 DOI: 10.1016/j.vetimm.2019.109939
Keywords: MERS-CoV; Monoclonal antibodies; Diagnostic tests.