[Source: Clinical Infectious Diseases, full page: (LINK). Abstract, edited.]
A Confirmed Case of SARS-CoV-2 Pneumonia with Routine RT-PCR Negative and Virus Variation in Guangzhou, China
Zhengtu Li, Yinhu Li, Lingdan Chen, Shaoqiang Li, Le Yu, Airu Zhu, Feng Yang, Qian Jiang, Liyan Chen, Jincun Zhao, Wenju Lu, Nanshan Zhong, Feng Ye
Clinical Infectious Diseases, ciaa941, https://doi.org/10.1093/cid/ciaa941
Published: 09 July 2020
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine RT-PCR detection of SARS-CoV-2. However, we report a confirmed case of SARS-CoV-2 pneumonia with routine RT-PCR negative. This case has been finally diagnosed by Nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variation of SARS-CoV-2 were found. This case has highlighted that false negative results could be present in the routine RT-PCR diagnosis, especially with virus variation. At the moment, Nanopore pathogen sequencing and antibody detection have been found effective in clinical diagnosis.
SARS-CoV-2, Pneumonia, Routine RT-PCR, Virus variation, Nanopore sequencing
Issue Section: Major Article
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Keywords: SARS-CoV-2; COVID-19; Diagnostic tests.