#Polymicrobial Nature of #Tick-Borne #Diseases (mBio, abstract)

[Source: mBio, full page: (LINK). Abstract, edited.]

Polymicrobial Nature of Tick-Borne Diseases

Santiago Sanchez-Vicente, Teresa Tagliafierro, James L. Coleman, Jorge L. Benach, Rafal Tokarz

Liise-anne Pirofski, Editor

DOI: 10.1128/mBio.02055-19

 

ABSTRACT

Tick-borne diseases have doubled in the last 12 years, and their geographic distribution has spread as well. The clinical spectrum of tick-borne diseases can range from asymptomatic to fatal infections, with a disproportionate incidence in children and the elderly. In the last few years, new agents have been discovered, and genetic changes have helped in the spread of pathogens and ticks. Polymicrobial infections, mostly in Ixodes scapularis, can complicate diagnostics and augment disease severity. Amblyomma americanum ticks have expanded their range, resulting in a dynamic and complex situation, possibly fueled by climate change. To document these changes, using molecular biology strategies for pathogen detection, an assessment of 12 microbes (9 pathogens and 3 symbionts) in three species of ticks was done in Suffolk County, New York. At least one agent was detected in 63% of I. scapularis ticks. Borrelia burgdorferi was the most prevalent pathogen (57% in adults; 27% in nymphs), followed by Babesia microti (14% in adults; 15% in nymphs), Anaplasma phagocytophilum (14% in adults; 2% in nymphs), Borrelia miyamotoi (3% in adults), and Powassan virus (2% in adults). Polymicrobial infections were detected in 22% of I. scapularis ticks, with coinfections of B. burgdorferi and B. microti (9%) and of B. burgdorferi and A. phagocytophilum (7%). Three Ehrlichia species were detected in 4% of A. americanum ticks. The rickettsiae constituted the largest prokaryotic biomass of all the ticks tested and included Rickettsia amblyommatis, Rickettsia buchneri, and Rickettsia montanensis. The high rates of polymicrobial infection in ticks present an opportunity to study the biological interrelationships of pathogens and their vectors.

 

IMPORTANCE

Tick-borne diseases have increased in prevalence in the United States and abroad. The reasons for these increases are multifactorial, but climate change is likely to be a major factor. One of the main features of the increase is the geographic expansion of tick vectors, notably Amblyomma americanum, which has brought new pathogens to new areas. The clinical spectrum of tick-borne diseases can range from asymptomatic to fatal infections, with a disproportionate incidence in children and the elderly. In addition, new pathogens that are cotransmitted by Ixodes scapularis have been discovered and have led to difficult diagnoses and to disease severity. Of these, Borrelia burgdorferi, the agent of Lyme disease, continues to be the most frequently transmitted pathogen. However, Babesia microti, Borrelia miyamotoi (another spirochete), Anaplasma phagocytophilum, and Powassan virus are frequent cotransmitted agents. Polymicrobial infection has important consequences for the diagnosis and management of tick-borne diseases.

Keywords: Tick-borne diseases; Powassan virus; Borrelia burgorferi; Infectious diseases.

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Essential Role of #Interferon Response in Containing #Human #Pathogenic #Bourbon Virus (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 25, Number 7—July 2019 / Research

Essential Role of Interferon Response in Containing Human Pathogenic Bourbon Virus

Jonas Fuchs, Tobias Straub, Maximilian Seidl, and Georg Kochs

Author affiliations: University of Freiburg Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany

 

Abstract

Bourbon virus (BRBV) is a recently discovered tick-transmitted viral pathogen that is prevalent in the Midwest and southern United States. Since 2014, zoonotic BRBV infections have been verified in several human cases of severe febrile illness, occasionally with fatal outcomes, indicating a possible public health threat. We analyzed the pathology of BRBV infection in mice and found a high sensitivity of the virus to the host interferon system. Infected standard laboratory mice did not show clinical signs or virus replication. However, in mice carrying defects in the type I and type II interferon system, the virus grew to high titers and caused severe pathology. In cell culture, BRBV was blocked by antiviral agents like ribavirin and favipiravir (T705). Our data suggest that persons having severe BRBV infection might have a deficiency in their innate immunity and could benefit from an already approved antiviral treatment.

Keywords: Arbovirus; Bourbon virus; Tick-borne infections; Viral pathogenesis; Antivirals; Interferons; Ribavirin; Favipiravir.

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#Lyme #neuroborreliosis‐ clinical outcomes, #controversy, #pathogenesis, and polymicrobial #infections (Ann Neurol., abstract)

[Source: Annals of Neurology, full page: (LINK). Abstract, edited.]

Lyme neuroborreliosis‐ clinical outcomes, controversy, pathogenesis, and polymicrobial infections

Juan Carlos Garcia‐Monco MD, PhD, FAAN,  Jorge L. Benach PhD

First published: 10 December 2018 / DOI: https://doi.org/10.1002/ana.25389

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.

Please cite this article as doi: 10.1002/ana.25389.

 

ABSTRACT

Lyme Borreliosis is the object of numerous misconceptions. In this review, we revisit the fundamental manifestations of neuroborreliosis (meningitis and cranial and radiculoneuritis) as these have withstood the test of time. We also discuss other manifestations that are less frequent. Stroke, as a manifestation of Lyme neuroborreliosis, is considered in the context of other infections. The summary of the literature regarding clinical outcomes of neuroborreliosis leads to its controversies. We also include new information on pathogenesis, and on the polymicrobial nature of tick‐borne diseases. In this way, we update the review that we wrote in this journal in 1995.

This article is protected by copyright. All rights reserved.

Keywords: Lyme’s Disease; Tick-borne diseases; Lyme Neuroborreliosis.

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#Tickborne #Diseases — Confronting a Growing #Threat (N Engl J Med., excerpt)

[Source: The New England Journal of Medicine, full page: (LINK). Excerpt, edited.]

Perspective

Tickborne Diseases — Confronting a Growing Threat

Catharine I. Paules, M.D., Hilary D. Marston, M.D., M.P.H., Marshall E. Bloom, M.D., and Anthony S. Fauci, M.D.

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Every spring, public health officials prepare for an upsurge in vectorborne diseases. As mosquito-borne illnesses have notoriously surged in the Americas, the U.S. incidence of tickborne infections has risen insidiously, triggering heightened attention from clinicians and researchers.

(…)

This article was published on July 25, 2018, at NEJM.org.

 

Author Affiliations

From the Office of the Director, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, Bethesda, MD (C.I.P., H.D.M., A.S.F.); and the Rocky Mountain Laboratories of the Intramural Research Program of NIAID, Hamilton, MT (M.E.B.).

Keywords: Emerging Diseases; Tick-borne diseases.

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#Rickettsia helvetica in #Human-Parasitizing and Free-Living #Ixodes ricinus from Urban and Wild Green Areas in the Metropolitan City of #Rome, #Italy (Vector Borne Zoo Dis., abstract)

[Source: Vector-Borne and Zoonotic Diseases, full page: (LINK). Abstract, edited.]

Rickettsia helvetica in Human-Parasitizing and Free-Living Ixodes ricinus from Urban and Wild Green Areas in the Metropolitan City of Rome, Italy

Scarpulla Manuela, Barlozzari Giulia, Salvato Laura, De Liberato Claudio, Lorenzetti Raniero, and Macrì Gladia

Published Online: 17 Apr 2018 / DOI: https://doi.org/10.1089/vbz.2017.2235

 

Abstract

Rickettsia helvetica is an emerging human pathogen, belonging to the spotted fever group (SFG) rickettsiae, associated with generally aneruptive fever, meningitis, and sudden death in chronic perimyocarditis. In this study, we describe the detection of R. helvetica in human-parasitizing and free-living Ixodes ricinus from the Metropolitan City of Rome. The pathogen was found in a tick acquired by a woman in an urban park. The circulation of R. helvetica was further confirmed by its detection in free-living ticks from a wild green area. These findings demonstrate that urban as well as wild green areas can represent a risk of infection to humans by R. helvetica, with potentially severe sequelae. To the best of our knowledge, this is the first report of R. helvetica in the Lazio region. Large-scale studies are needed to evaluate and quantify the presence of R. helvetica and other SFG rickettsiae in the urban and periurban context and to assess the risk to humans and animals related to their frequentation.

Keywords:  Rickettsia Helvetica; Italy; Ticks.

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Molecular #genomic characterization of #tick- and #human-derived #SFTS virus isolates from #SouthKorea

[Source: PLoS Neglected Tropical Diseases, full page: (LINK). Abstract, edited.]

OPEN ACCESS /  PEER-REVIEWED / RESEARCH ARTICLE

Molecular genomic characterization of tick- and human-derived severe fever with thrombocytopenia syndrome virus isolates from South Korea

Seok-Min Yun , Su-Jin Park , Sun-Whan Park, WooYoung Choi, Hye Won Jeong, Young-Ki Choi , Won-Ja Lee

Published: September 22, 2017 / DOI: https://doi.org/10.1371/journal.pntd.0005893 / This is an uncorrected proof.

 

Abstract

Background

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne viral disease caused by the SFTS virus (SFTSV) from Bunyaviridae that is endemic in East Asia. However, the genetic and evolutionary characteristics shared between tick- and human-derived Korean SFTSV strains are still limited.

Methodology/Principal findings

In this study we identify, for the first time, the genome sequence of a tick (Haemaphysalis longicornis)-derived Korean SFTSV strain (designated as KAGWT) and compare this virus with recent human SFTSV isolates to identify the genetic variations and relationships among SFTSV strains. The genome of the KAGWT strain is consistent with the described genome of other members of the genus Phlebovirus with 6,368 nucleotides (nt), 3,378 nt, and 1,746 nt in the Large (L), Medium (M) and Small (S) segments, respectively. Compared with other completely sequenced human-derived Korean SFTSV strains, the KAGWT strain had highest sequence identities at the nucleotide and deduced amino acid level in each segment with the KAGWH3 strain which was isolated from SFTS patient within the same region, although there is one unique amino acid substitution in the Gn protein (A66S). Phylogenetic analyses of complete genome sequences revealed that at least four different genotypes of SFTSV are co-circulating in South Korea, and that the tick- and human-derived Korean SFTSV strains (genotype B) are closely related to one another. Although we could not detect reassortant, which are commonly observed in segmented viruses, further large-scale surveillance and detailed genomic analysis studies are needed to better understand the molecular epidemiology, genetic diversity, and evolution of SFTSV.

Conclusions/Significance

Full-length sequence analysis revealed a clear association between the genetic origins of tick- and human-derived SFTSV strains. While the most prevalent Korean SFTSV is genotype B, at least four different genotypes of SFTSV strains are co-circulating in South Korea. These findings provide information regarding the molecular epidemiology, genetic diversity, and evolution of SFTSV in East Asia.

 

Author summary

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne viral disease caused by the SFTS virus (SFTSV). During entomological surveillance of SFTSV infection in Korean ticks collected from SFTS outbreak areas, we isolated a single SFTSV strain which we designated KAGWT. In addition, we isolated three SFTSVs from human patients with typical SFTS symptoms. In this study, we report the genomic sequences of each of these isolates and compare the genetic and evolutionary characteristics between tick- and human-derived Korean SFTSV isolates. Genetic and phylogenetic analyses of these sequences revealed that the tick-derived Korean SFTSV strain is clustered into genotype B, the most prevalent genotype in South Korea, and was closely related to other SFTSV in the same group. Furthermore, our results show that at least four different genotypes of SFTSV strains are co-circulating in South Korea.

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Citation: Yun S-M, Park S-J, Park S-W, Choi W, Jeong HW, Choi Y-K, et al. (2017) Molecular genomic characterization of tick- and human-derived severe fever with thrombocytopenia syndrome virus isolates from South Korea. PLoS Negl Trop Dis 11(9): e0005893. https://doi.org/10.1371/journal.pntd.0005893

Editor: Masayuki Saijo, National Institute of Infectious Disease, JAPAN

Received: May 10, 2017; Accepted: August 22, 2017; Published: September 22, 2017

Copyright: © 2017 Yun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the paper and its Supporting Information files, and deposited in the GenBank database with accession numbers KY273136-KY273138 and KY789433-KY789441.

Funding: This research was funded by intramural grants of the Korea National Research Institute of Health (grant numbers: 2014-ND53001 and 2017-NI53002-00) and a grant of the Korea Health Industry Development Institute (grant number: HI15C2817). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Keywords: STFS; South Korea.

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#Serologic #Evidence of #Powassan Virus #Infection in Patients with Suspected #Lyme Disease (@CDC_EIDjournal, abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 23, Number 8—August 2017 / Dispatch

Serologic Evidence of Powassan Virus Infection in Patients with Suspected Lyme Disease

Holly M. Frost1, Anna M. Schotthoefer, Angela M. Thomm, Alan P. Dupuis, Sue C. Kehl, Laura D. Kramer, Thomas R. Fritsche, Yvette A. Harrington, and Konstance K. Knox

Author affiliations: Marshfield Clinic Research Foundation, Marshfield, Wisconsin, USA (H.M. Frost, A.M. Schotthoefer, T.R. Fritsche); Coppe Laboratories, Waukesha, Wisconsin, USA (A.M. Thomm, Y.A. Harrington, K.K. Knox); New York State Department of Health, Slingerlands, New York, USA (A.P. Dupuis II, L.D. Kramer); Medical College of Wisconsin, Milwaukee, Wisconsin, USA (S.C. Kehl)

 

Abstract

Powassan virus (POWV) lineage II is an emerging tickborne flavivirus with an unknown seroprevalence in humans. In a Lyme disease–endemic area, we examined the seroreactivity to POWV in 2 patient cohorts and described the clinical features of the POWV-seroreactive patients. POWV disease might be less neuroinvasive than previously thought.

Keywords: Powassan Encephalitis Virus; Tick Borne Infections; Ticks; Lyme Disease; Flavivirus.

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#SFTSV #Infection in #Minks in #China (Vector Borne Zoo Dis., abstract)

[Source: Vector-Borne and Zoonotic Diseases, full page: (LINK). Abstract, edited.]

Vector-Borne and Zoonotic Diseases

Severe Fever with Thrombocytopenia Syndrome Virus Infection in Minks in China

To cite this article: Wang Gui-sheng, Wang Jin-bao, Tian Fu-lin, Zhang Hua-jie, Yin Fei-fei, Xu Cong, Xu Dong, Huang Yu-ting, and Yu Xue-jie. Vector-Borne and Zoonotic Diseases. June 2017, ahead of print. https://doi.org/10.1089/vbz.2017.2115

Online Ahead of Print: June 27, 2017

Author information: Gui-sheng Wang,1,2 Jin-bao Wang,1 Fu-lin Tian,2 Hua-jie Zhang,3 Fei-fei Yin,3 Cong Xu,2 Dong Xu,3 Yu-ting Huang,4 and Xue-jie Yu5,6

1School of Life Sciences, Shandong University, Jinan, China. 2Shandong Provincial Center for Animal Disease Control and Prevention, Jinan, China. 3Weihai City Center for Animal Disease Control and Prevention, Weihai, China. 4School of Public Health, Shandong University, Jinan, China. 5Wuhan University School of Health Sciences, Wuhan, China.  6Department of Pathology, University of Texas Medical Branch, Galveston, Texas.

Address correspondence to: Xue-jie Yu, Wuhan University School of Health Sciences, Wuhan City 430071, Hubei Province, China, E-mail: xuyu@utmb.edu

 

ABSTRACT

We analyzed the seroprevalence of tick-borne severe fever with thrombocytopenia syndrome virus (SFTSV) in farm-raised minks using double antigen ELISA (enzyme-linked immunosorbent assay) kit and indicated that 8.4% (15/178) of the minks had antibodies to the nucleoprotein of SFTSV and 72.7% (8/11) of mink farms had minks positive to SFTSV. The ELISA results were further confirmed by presence of neutralization to SFTSV in the mink sera. Our results suggested that minks were widely infected with SFTSV in China.

Keywords: SFTS; Tick-Borne Infections; Vector-borne infections; Wildlife; China.

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#CCHF Virus and #Alkhurma (Alkhumra) Virus in #Ticks in #Djibouti (Vector Borne Zoo Dis., abstract)

[Source: Vector Borne and Zoonotic Diseases, full page: (LINK). Abstract, edited.]

Vector-Borne and Zoonotic Diseases

Crimean Congo Hemorrhagic Fever Virus and Alkhurma (Alkhumra) Virus in Ticks in Djibouti

To cite this article: Horton Katherine C., Fahmy Nermeen T., Watany Noha, Zayed Alia, Mohamed Abro, Ahmed Ammar Abdo, Rollin Pierre E., and Dueger Erica L..

Vector-Borne and Zoonotic Diseases. August 2016, ahead of print. doi:10.1089/vbz.2016.1951.

Online Ahead of Print: August 10, 2016

Author information: Katherine C. Horton,1 Nermeen T. Fahmy,2 Noha Watany,2 Alia Zayed,2 Abro Mohamed,3 Ammar Abdo Ahmed,3 Pierre E. Rollin,4 and Erica L. Dueger1,5

1Global Disease Detection Program, U.S. Centers for Disease Control and Prevention, Cairo, Egypt. 2Vector Biology Research Program, U.S. Naval Medical Research Unit No. 3, Cairo, Egypt. 3Department of Epidemiology and Information Services, Ministry of Health, Djibouti, Djibouti. 4Viral Special Pathogens Branch, National Center for Emerging and Zoonotic Infectious Diseases, U.S. Centers for Disease Control and Prevention, Atlanta, Georgia. 5Global Disease Detection Branch, Division of Global Health Protection, Center for Global Health, U.S. Centers for Disease Control and Prevention, Atlanta, Georgia.

Address correspondence to: Katherine C. Horton, Global Disease Detection Program, U.S. Centers for Disease Control and Prevention, PSC 452 Box 5000, FPO, AE 09835, United States, E-mail: katherinehorton12@gmail.com

 

ABSTRACT

Crimean Congo Hemorrhagic Fever virus and Alkhumra virus, not previously reported in Djibouti, were detected among 141 (infection rate = 15.7 per 100, 95% CI: 13.4–18.1) tick pools from 81 (37%) cattle and 2 (infection rate = 0.2 per 100, 95% CI: 0.0–0.7) tick pools from 2 (1%) cattle, respectively, collected at an abattoir in 2010 and 2011.

Keywords: Research; Abstracts; CCHF; Ticks; Alkhurma Virus; Djibouti.

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#Detection of #SFTS #Virus from Wild #Animals and Ixodidae #Ticks in the Republic of #Korea (Vector Borne Zoo Dis., abstract)

[Source: Vector Borne and Zoonotic Diseases, full page: (LINK). Abstract, edited.]

Vector-Borne and Zoonotic Diseases

Detection of Severe Fever with Thrombocytopenia Syndrome Virus from Wild Animals and Ixodidae Ticks in the Republic of Korea

To cite this article: Oh Sung-Suck, Chae Jeong-Byoung, Kang Jun-Gu, Kim Heung-Chul, Chong Sung-Tae, Shin Jeong-Hwa, Hur Moon-Suk, Suh Jae-Hwa, Oh Myoung-Don, Jeong Soo-Myoung, Shin Nam-Shik, Choi Kyoung-Seong, and Chae Joon-Seok. Vector-Borne and Zoonotic Diseases. April 2016, ahead of print. doi:10.1089/vbz.2015.1848.

Online Ahead of Print: April 4, 2016

Author information: Sung-Suck Oh,1,2 Jeong-Byoung Chae,1 Jun-Gu Kang,1 Heung-Chul Kim,3 Sung-Tae Chong,3 Jeong-Hwa Shin,4 Moon-Suk Hur,4 Jae-Hwa Suh,4 Myoung-Don Oh,5 Soo-Myoung Jeong,6 Nam-Shik Shin,7 Kyoung-Seong Choi,8 and Joon-Seok Chae1

1Laboratory of Veterinary Internal Medicine, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea. 2Incheon Metropolitan City Institute of Health and Environment, Incheon, Republic of Korea. 35th Medical Detachment, 168th Multifunctional Medical Battalion, 65th Medical Brigade, Seoul, Republic of Korea. 4National Institute of Environmental Research, Incheon, Republic of Korea. 5Seoul National University College of Medicine, Seoul, Republic of Korea. 6Biological Diversity Division, Nature Conservation Bureau, Ministry of Environment, Sejong, Republic of Korea. 7Laboratory of Zoo and Wildlife Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea. 8Department of Animal Biotechnology, Kyungpook National University, Sangju, Republic of Korea.

Address correspondence to: Joon-seok Chae, Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea, E-mail: jschae@snu.ac.kr

 

ABSTRACT

Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV), a novel bunyavirus reported to be endemic to central-northeastern China, southern Japan, and the Republic of Korea (ROK). To investigate SFTSV infections, we collected serum samples and ticks from wild animals. Using serum samples and ticks, SFTSV-specific genes were amplified by one-step RT-PCR and nested PCR and sequenced. Indirect immunofluorescence assay (IFA) was performed to analyze virus-specific antibody levels in wild animals. Serum samples were collected from a total of 91 animals: 21 Korean water deer (KWD), 3 Siberian roe deer, 5 gorals, 7 raccoon dogs, 54 wild boars (WBs), and 1 carrion crow. The SFTSV infection rate in wild animals was 3.30% (3 of 91 animals: 1 KWD and 2 WBs). The seropositive rate was 6.59% (6 of 91 animals: 5 KWD and 1 WB). A total of 891 ticks (3 species) were collected from 65 wild animals (9 species). Of the attached tick species, Haemaphysalis longicornis (74.86%) was the most abundant, followed by Haemaphysalis flava (20.20%) and Ixodes nipponensis (4.94%). The average minimum infection rate (MIR) of SFTSV in ticks was 4.98%. The MIRs of H. longicornis, H. flava, and I. nipponensis were 4.51%, 2.22%, and 22.73%, respectively. The MIRs of larvae, nymphs, and adult ticks were 0.68%, 6.88%, and 5.53%, respectively. In addition, the MIRs of fed and unfed ticks were 4.67% and 4.96%, respectively. We detected a low SFTSV infection rate in wild animals, no differences in SFTSV infection rate with respect to bloodsucking in ticks, and SFTSV infection for all developmental stages of ticks. This is the first report describing the detection of SFTSV in wild animals in the ROK.

Keywords: Research; Abstracts; SFTS; Wildlife; Ticks; S. Korea.

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