A novel #reassortant #influenza A (#H1N1) virus #infection in #swine in #Shandong Province, eastern #China (Transbound Emerg Dis., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Transbound Emerg Dis. 2019 Sep 19. doi: 10.1111/tbed.13360. [Epub ahead of print]

A novel reassortant influenza A (H1N1) virus infection in swine in Shandong Province, eastern China.

Yu Z1,2,3, Cheng K4, He H5, Wu J1,2,3.

Author information: 1 Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan, 250023, China. 2 Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology. 3 Poultry Breeding Engineering Technology Center of Shandong Province. 4 Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, 250132, China. 5 College of Life Sciences, Shandong Normal University, Jinan, 250014, China.



Influenza A (H1N1) viruses are distributed worldwide and pose a threat to public health. Swine, as a natural host and mixing vessel of influenza A (H1N1) virus, play a critical role in the transmission of this virus to humans. Furthermore, swine influenza A (H1N1) viruses have provided all eight genes or some genes to the genomes of influenza strains that historically have caused human pandemics. Hence, persistent surveillance of influenza A (H1N1) virus in swine herds could contribute to the prevention and control of this virus. Here, we report a novel reassortant influenza A (H1N1) virus generated by reassortment between 2009 pandemic H1N1 viruses and swine viruses. We also found that this virus is prevalent in swine herds in Shandong Province, eastern China. Our findings suggest that surveillance of the emergence of the novel reassortant influenza A (H1N1) virus in swine is imperative.

© 2019 Blackwell Verlag GmbH.

KEYWORDS: H1N1; human; influenza; reassortant; swine

PMID: 31535780 DOI: 10.1111/tbed.13360

Keywords: Seasonal Influenza; Swine Influenza; H1N1; H1N1pdm09; Pigs; Reassortant strain; Shandong; China.



Detection of #plasmid-mediated #tigecycline-resistant gene tet(X4) in #Escherichia coli from #pork, #Sichuan and #Shandong Provinces, #China, February 2019 (Euro Surveill., abstract)

[Source: Eurosurveillance, full page: (LINK). Abstract, edited.]

Detection of plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from pork, Sichuan and Shandong Provinces, China, February 2019

Li Bai1,2,3, Pengcheng Du3,4, Yinju Du5, Honghu Sun1,2,6, Pei Zhang1,2, Yuping Wan6, Qi Lin6, Séamus Fanning1,2,7, Shenghui Cui8, Yongning Wu1,2

Affiliations: 1 Key Laboratory of Food Safety Risk Assessment, National Health Commission of the People’s Republic of China, Beijing, People’s Republic of China; 2 Food Safety Research Unit of Chinese Academy of Medical Sciences, China National Center for Food Safety Risk Assessment, Beijing, People’s Republic of China; 3 These authors contributed equally to this work; 4 Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, and Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, People’s Republic of China; 5 Center for disease control and prevention of Liaocheng city, Liaocheng, People’s Republic of China; 6 Chengdu institute for Food and Drug Control, Chengdu, People’s Republic of China; 7 UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, University College Dublin, Belfield, Dublin, Ireland; 8 Department of Food Science, National Institutes for Food and Drug Control, Beijing, People’s Republic of China

Correspondence:  Yongning Wu

Citation style for this article: Bai Li, Du Pengcheng, Du Yinju, Sun Honghu, Zhang Pei, Wan Yuping, Lin Qi, Fanning Séamus, Cui Shenghui, Wu Yongning. Detection of plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from pork, Sichuan and Shandong Provinces, China, February 2019. Euro Surveill.2019;24(25):pii=1900340. https://doi.org/10.2807/1560-7917.ES.2019.24.25.1900340

Received: 29 May 2019;   Accepted: 20 Jun 2019



The plasmid-mediated high-level tigecycline resistance gene, tet(X4), was detected in seven Escherichia coli isolates from pork in two Chinese provinces. Two isolates belonged to the epidemic spreading sequence type ST101. Tet(X4) was adjacent to ISVsa3 and concurrent with floR in all seven isolates. In addition to IncFIB, the replicon IncFII was found to be linked to tet(X4). This report follows a recent detection of tet(X3)/(X4) in E. coli from animals and humans in China.

©  This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords: Antibiotics; Drugs Resistance; Tigecycline; Plasmids; Pigs; E. Coli; Food Safety; China; Sichuan; Shandong.


The characteristics of current natural foci of #HFRS in #Shandong Province, #China, 2012-2015 (PLoS Negl Trop Dis., abstract)

[Source: PLoS Neglected Tropical Diseases, full page: (LINK). Abstract, edited.]


The characteristics of current natural foci of hemorrhagic fever with renal syndrome in Shandong Province, China, 2012-2015

Zhaolei Zheng, Peizhu Wang, Zhiqiang Wang, Dandan Zhang, Xu Wang, Shuqing Zuo, Xiujun Li

Published: May 20, 2019 / DOI: https://doi.org/10.1371/journal.pntd.0007148 / This is an uncorrected proof.




Hemorrhagic fever with renal syndrome (HFRS), an infectious disease caused by hantaviruses, is endemic in China and remains a serious public health problem. Historically, Shandong Province has had the largest HFRS burden in China. However, we do not have a comprehensive and clear understanding of the current epidemic foci of HFRS in Shandong Province.

Methodology/principal findings

The incidence and mortality rates were calculated, and a phylogenetic analysis was performed after laboratory testing of the virus in rodents. Spatial epidemiology analysis was applied to investigate the epidemic foci, including their sources. A total of 6,206 HFRS cases and 59 related deaths were reported in Shandong Province. The virus carriage rates of the rodents Rattus norvegicus, Apodemus agrarius and Mus musculus were 10.24%, 6.31% and 0.27%, respectively. The phylogenetic analysis indicated that two novel viruses obtained from R. norvegicus in Anqiu City and Qingzhou City were dissimilar to the other strains, but closely related to strains previously isolated in northeastern China. Three epidemic foci were defined, two of which were derived from the Jining and Linyi epidemic foci, respectively, while the other was the residue of the Jining epidemic focus.


The southeastern and central Shandong Province are current key HFRS epidemic foci dominated by A. agrarius and R. norvegicus, respectively. Our study could help local departments to strengthen prevention and control measures in key areas to reduce the hazards of HFRS.


Author summary

Hemorrhagic fever with renal syndrome (HFRS) is a global infectious disease, which is still a serious public health threat in China today. The reported HFRS cases in Shandong Province accounted for approximate one third of total cases in the whole country. HFRS is a zoonosis mainly caused by Hantaan virus (HTNV) and Seoul virus (SEOV), which natural rodent hosts are A. agrarius and R. norvegicus, respectively. To explore the current HFRS epidemic foci based on patients, rodents and molecular epidemiology characteristics in Shandong Province, we collected the records of HFRS cases from whole province and the rodents captured in 14 surveillance sites. We found that the epidemic situation of HFRS is quiet different in temporal and spatial distribution. Three epidemic foci were defined based on patients, rodents and molecular epidemiology characteristics. The situation of HFRS epidemic foci in Shandong Province was clear. Our study provides a reference for relevant departments to develop key prevention strategies.


Citation: Zheng Z, Wang P, Wang Z, Zhang D, Wang X, Zuo S, et al. (2019) The characteristics of current natural foci of hemorrhagic fever with renal syndrome in Shandong Province, China, 2012-2015. PLoS Negl Trop Dis 13(5): e0007148. https://doi.org/10.1371/journal.pntd.0007148

Editor: David Joseph Diemert, George Washington University School of Medicine and Health Sciences, UNITED STATES

Received: January 3, 2019; Accepted: May 2, 2019; Published: May 20, 2019

Copyright: © 2019 Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: Some relevant data are within the manuscript and its Supporting Information files. The data of HFRS incidence and death underlying the results presented in the study are available from Shandong Provincial Center for Disease Control and Prevention (http://www.sdcdc.cn/col/col9776/index.html?uid=51587&pageNum=1).

Funding: This research was supported by the Natural Science Foundation of China (no. 81673238, 81473025), the Natural Science Foundation of Shandong Province (no. ZR2016HM75) and State Key Research Development Program of China (2016YFC1201902). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Keywords: Hantavirus; HFRS; Shandong; China.


Novel #astroviruses types circulating in #Shandong Province (Eastern #China) during 2016: A clinical and #environmental #surveillance (J Clin Virol., abstract)

[Source: Journal of Clinical Virology, full page: (LINK). Summary, edited.]

Journal of Clinical Virology / Available online 12 May 2019 / In Press, Accepted Manuscript  / Short communication

Novel astroviruses types circulating in Shandong Province (Eastern China) during 2016: A clinical and environmental surveillance

Zexin Tao a, Haiyan Wang a, Wenqiang Zhang a, Aiqiang Xu a,b

{a} Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, 16992 Jingshi Road, Jinan 250014, People’s Republic of China; {b} School of Public Health, Shandong University, No. 44 Wenhuaxi Road, Jinan 250012, People’s Republic of China

Received 13 December 2018, Revised 29 April 2019, Accepted 11 May 2019, Available online 12 May 2019. DOI: https://doi.org/10.1016/j.jcv.2019.05.005



  • Eighteen of 635 specimens (2.8%) were positive for novel astrovirus with 13 MLB1, 4 MLB2, and 1 VA3 in Jinan, China in 2016.
  • Novel astrovirus subtypes in sewage include MLB1, MLB2, VA1, VA2, VA3, and VA5.
  • VA2, MLB1, and VA1 were the most common subtypes in sewage samples.
  • Eighteen partial ORF2 sequences that could not be classified into any known novel astrovirus subtypes were identified from sewage samples.


Keywords: Astrovirus; Shandong; China.


Neutralizing #antibodies to #SFTS Virus in general #population, #Shandong Province, #China (Sci Rep., abstract)

[Source: Scientific Reports, full page: (LINK). Abstract, edited.]

Article | OPEN | Published: 18 October 2018

Neutralizing antibodies to Severe Fever with Thrombocytopenia Syndrome Virus in general population, Shandong Province, China

Dexin Li,  Lijun Shao,  Yu Bi &  Guoyu Niu

Scientific Reports, volume 8, Article number: 15401 (2018)



Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS virus (SFTSV) in East Asia. The research on seroprevalence of SFTSV in healthy people and risk factors had been detailed. However, the levels of neutralizing antibodies against SFTSV in general population were currently unclear. In the present study, we tested 1375 healthy persons from Penglai County, eastern China, for SFTSV neutralizing antibodies; 0.58% (8/1,375) was positive and the positive rates were not significantly different among people at different age groups, occupations and genders. Besides, a follow-up study was conducted and the titer of neutralizing antibodies decreased over time in all eight people but one, and the neutralizing antibodies of five lasted for the entire study period of seven years. Our results suggesting that subclinical infection or a relatively mild form of SFTS illness is occurring in this population, but a small percentage of sera have neutralizing capacity to SFTSV. Hence, most people are just susceptible to SFTSV infection.

Keywords: SFTS; Neutralizing antibodies; Seroprevalence; China; Shandong.


#Antibiotic use in people and #pigs: a #OneHealth survey of rural residents’ knowledge, attitudes and practices in #Shandong province, #China (J Antimicrob Chemother., abstract)

[Source: Journal of Antimicrobial Chemotherapy, full page: (LINK). Abstract, edited.]

Antibiotic use in people and pigs: a One Health survey of rural residents’ knowledge, attitudes and practices in Shandong province, China

Oliver J Dyar, Jia Yin, Lilu Ding, Karin Wikander, Tianyang Zhang, Chengtao Sun, Yang Wang, Christina Greko, Qiang Sun, Cecilia Stålsby Lundborg

Journal of Antimicrobial Chemotherapy, dky240, https://doi.org/10.1093/jac/dky240

Published: 23 July 2018




To evaluate rural residents’ knowledge, attitudes and practices (KAP) towards antibiotic use in humans and pigs, among individuals with and without backyard pig farms living in Shandong province, China.


We conducted a cross-sectional questionnaire study among residents in 12 villages, and directly observed medicines stored in households for humans and pigs.


In total, 769 residents participated, including 330 backyard pig farmers. Respondents had low levels of knowledge about antibiotics. A quarter of participants had bought one or more antibiotics from a pharmacy without a prescription in the previous year, and this was more common among pig farmers who had bought antibiotics for their pigs without consulting a vet (49% versus 25%, P < 0.001). Stored antibiotics for human use were found in 42% of households, and 70% of participants from these households did not know they were storing antibiotics. Thirty-one percent of backyard pig farmers were storing antibiotics for pig use. Farmers who thought it was good to store leftover antibiotics for their pigs were more likely to have stored antibiotics for pigs (41% versus 20%) and for humans (47% versus 32%; both P < 0.01). A fifth of participants thought their own actions were important for controlling antibiotic resistance.


We found differences in the KAP of backyard pig farmers and non-pig farmers to antibiotics, and parallels between pig farmers’ attitudes and behaviours towards antibiotic use in pigs and in humans. Our findings reinforce the need for context-adapted multifaceted interventions to improve antibiotic use and provide suggestions for targeting educational approaches.

Topic: antibiotics – attitude – china – internship and residency – suidae – medical residencies

Keywords: Antibiotics; Human; China; Pigs; Society.


#Seroprevalence of #SFTSV in #Hedgehog from #China (Vector Borne Zoo Dis., abstract)

[Source: Vector Borne and Zoonotic Diseases, full page: (LINK). Abstract, edited.]

Vector-Borne and Zoonotic Diseases

Seroprevalence of Severe Fever with Thrombocytopenia Syndrome Virus in Hedgehog from China

To cite this article: Sun Yue, Liu Miao-miao, Luo Li-mei, Zhao Li, Wen Hong-Ling, Zhang Zhen-Tang, Liu Jian-Wei, Xue Zai-Feng, Ma Dong-Qiang, Ding Shu-Jun, Lei Xiao-Ying, and Yu Xue-jie. Vector-Borne and Zoonotic Diseases. March 2017, ahead of print. doi:10.1089/vbz.2016.2019.

Online Ahead of Print: March 21, 2017

Author information: Yue Sun,1 Miao-miao Liu,1 Li-mei Luo,1 Li Zhao,1 Hong-Ling Wen,1 Zhen-Tang Zhang,2 Jian-Wei Liu,1 Zai-Feng Xue,2 Dong-Qiang Ma,2 Shu-Jun Ding,3 Xiao-Ying Lei,1 and Xue-jie Yu4

1School of Public Health, Shandong University, Jinan, China. 2Huangdao District Center for Disease Control and Prevention, Qingdao City, China. 3Shandong Province Center for Disease Control and Prevention, Jinan, China. 4School of Health Sciences, Wuhan University, Wuhan, China.

Address correspondence to: Xue-jie Yu, School of Health Sciences, Wuhan University, Wuhan, Hubei Province 430071, China, E-mail: yuxuejie@whu.edu.cn



Severe fever with thrombocytopenia syndrome, an emerging hemorrhagic fever, is caused by severe fever with thrombocytopenia syndrome virus (SFTSV), a tick-borne bunyavirus. Information regarding SFTSV animal hosts is very limited. In this study, we showed that 64% (9/14) of hedgehogs in Shandong Province, China were seropositive to SFTSV antibody, suggesting that hedgehog could be a vertebrate parasitifer for SFTSV.

Keywords: SFTS; China; Shandong; Hedgeohogs; Wildlife.