Low #Circulation of Subclade A1 #EVD68 Strains in #Senegal during 2014 North America #Outbreak (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 25, Number 7—July 2019 / Dispatch

Low Circulation of Subclade A1 Enterovirus D68 Strains in Senegal during 2014 North America Outbreak

Amary Fall, Mamadou Malado Jallow, Ousmane Kebe, Davy Evrard Kiori, Sara Sy, Déborah Goudiaby, Cheikh Saad Bouh Boye, Mbayame Ndiaye Niang1, and Ndongo Dia1

Author affiliations: Institute Pasteur de Dakar, Dakar, Senegal (A. Fall, M.M. Jallow, O. Kebe, D.E. Kiori, S. Sy, D. Goudiaby, M.N. Niang, N. Dia); Aristide Le Dantec Teaching Hospital, Dakar (C.S.B. Boye)

 

Abstract

To retrospectively investigate enterovirus D68 circulation in Senegal during the 2014 US outbreak, we retrieved specimens from 708 persons, mostly children, who had acute respiratory symptoms during September–December 2014. Enterovirus D68 was detected in 14 children (2.1%); most cases occurred in October. Phylogenetic analysis revealed that all strains clustered within subclade A1.

Keywords: Enterovirus; EV-D68; Senegal.

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#MERS #coronavirus infection in non-camelid domestic #mammals (Emerg Microbes Infect., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Emerg Microbes Infect. 2019;8(1):103-108. doi: 10.1080/22221751.2018.1560235.

Middle East respiratory syndrome coronavirus infection in non-camelid domestic mammals.

Kandeil A1, Gomaa M1, Shehata M1, El-Taweel A1, Kayed AE1, Abiadh A2, Jrijer J2, Moatasim Y1, Kutkat O1, Bagato O1, Mahmoud S1, Mostafa A1,3, El-Shesheny R1,4, Perera RA5, Ko RL5, Hassan N6, Elsokary B6, Allal L7, Saad A7, Sobhy H7, McKenzie PP4, Webby RJ4, Peiris M5, Ali MA1, Kayali G8,9.

Author information: 1 a Center of Scientific Excellence for Influenza Virus , National Research Centre , Giza , Egypt. 2 b Nature Link , Sfax , Tunisia. 3 c Institute of Medical Virology , Justus Liebig University Giessen , Giessen , Germany. 4 d St. Jude Children’s Research Hospital , Memphis , TN , USA. 5 e School of Public Health , University of Hong Kong , Sandy Bay , Hong Kong. 6 f General Organizations of Veterinary Services , Ministry of Agriculture and Land Reclamation , Giza , Egypt. 7 g Food and Agriculture Organization of the United Nations , Emergency Center for Transboundary Animal Diseases , Giza , Egypt. 8 h Human Link , Baabda , Lebanon. 9 i University of Texas Health Sciences Center , Houston , TX , USA.

 

Abstract

Dromedary camels are natural host of the Middle East respiratory syndrome coronavirus (MERS-CoV). However, there are limited studies of MERS-CoV infection of other domestic mammals exposed to infected dromedaries. We expanded our surveillance among camels in Egypt, Tunisia, and Senegal to include other domestic mammalian species in contact with infected camels. A total of 820 sera and 823 nasal swabs from cattle, sheep, goats, donkeys, buffaloes, mules, and horses were collected. Swabs were tested using RT-PCR and virus RNA-positive samples were genetically sequenced and phylogenetically analysed. Sera were screened using virus microneutralization tests and positive sera (where available) were confirmed using plaque reduction neutralization tests (PRNT). We detected 90% PRNT confirmed MERS-CoV antibody in 35 (55.6%) of 63 sera from sheep collected from Senegal, two sheep (1.8%) of 114 in Tunisia and a goat (0.9%) of 107 in Egypt, with titres ranging from 1:80 to ≥1:320. We detected MERS-CoV RNA in swabs from three sheep (1.2%) of 254 and five goats (4.1%) of 121 from Egypt and Senegal, as well as one cow (1.9%) of 53 and three donkeys (7.1%) of 42 from Egypt. Partial sequences of the RT-PCR amplicons confirmed specificity of the results. This study showed that domestic livestock in contact with MERS-CoV infected camels may be at risk of infection. We recommend expanding current MERS-CoV surveillance in animals to include other livestock in close contact with dromedary camels. The segregation of camels from other livestock in farms and live animal markets may need to be considered.

KEYWORDS: Egypt; MERS-CoV; Senegal; Tunisia; serology; sheep; surveillance

PMID: 30866764 DOI: 10.1080/22221751.2018.1560235

Keywords: MERS-CoV; Sheeps; Goats; Senegal; Tusinia; Egypt.

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#Serological #Survey of #Zoonotic #Viruses in Invasive and Native Commensal #Rodents in #Senegal, West Africa (Vector Borne Zoo Dis., abstract)

[Source: Vector-Borne and Zoonotic Diseases, full page: (LINK). Abstract, edited.]

Vector-Borne and Zoonotic Diseases

Serological Survey of Zoonotic Viruses in Invasive and Native Commensal Rodents in Senegal, West Africa

To cite this article: Diagne Christophe A., Charbonnel Nathalie, Henttonen Heikki, Sironen Tarja, and Brouat Carine. Vector-Borne and Zoonotic Diseases. September 2017, ahead of print. https://doi.org/10.1089/vbz.2017.2135

Online Ahead of Print: September 5, 2017

Author information: Christophe A. Diagne,1,2,3 Nathalie Charbonnel,4 Heikki Henttonen,5 Tarja Sironen,6 and Carine Brouat1

1 CBGP, IRD, CIRAD, INRA, Montpellier SupAgro, University of Montpellier, Montpellier, France. 2 BIOPASS (IRD-CBGP, ISRA, UCAD), Dakar, Senegal. 3 Département de Biologie Animale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop (UCAD), Dakar, Senegal. 4 CBGP, INRA, CIRAD, IRD, Montpellier SupAgro, University of Montpellier, Montpellier, France. 5 Forest and Animal Ecology, Natural Resources Institute Finland, Helsinki, Finland. 6 Department of Virology, University of Helsinki, Helsinki, Finland.

Address correspondence to: Christophe Diagne, Centre de Biologie pour la Gestion des Populations (CBGP)755 avenue du Campus Agropolis, Campus de Baillarguet CS 30016, 34988 Montferrier/Lez cedex, France, E-mail: chrisdiagne89@hotmail.fr

 

ABSTRACT

Increasing studies on rodent-borne diseases still highlight the major role of rodents as reservoirs of numerous zoonoses of which the frequency is likely to increase worldwide as a result of accelerated anthropogenic changes, including biological invasions. Such a situation makes pathogen detection in rodent populations important, especially in the context of developing countries characterized by high infectious disease burden. Here, we used indirect fluorescent antibody tests to describe the circulation of potentially zoonotic viruses in both invasive (Mus musculus domesticus and Rattus rattus) and native (Mastomys erythroleucus and Mastomys natalensis) murine rodent populations in Senegal (West Africa). Of the 672 rodents tested, we reported 22 seropositive tests for Hantavirus, Orthopoxvirus, and Mammarenavirus genera, and no evidence of viral coinfection. This study is the first to report serological detection of Orthopoxvirus in rodents from Senegal, Mammarenavirus in R. rattus from Africa, and Hantavirus in M. m. domesticus and in M. erythroleucus. Further specific identification of the viral agents highlighted here is urgently needed for crucial public health concerns.

Keywords: Serosurveys; Rodents; Senegal; Orthopoxvirus; Mammarenavirus; Hantavirus.

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