#Wolbachia pipientis occurs in #Aedes aegypti populations in #NM and #Florida, #USA (Ecol Evol., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Ecol Evol. 2019 Apr 26;9(10):6148-6156. doi: 10.1002/ece3.5198. eCollection 2019 May.

Wolbachia pipientis occurs in Aedes aegypti populations in New Mexico and Florida, USA.

Kulkarni A1, Yu W1, Jiang J1, Sanchez C1, Karna AK1, Martinez KJL1, Hanley KA1, Buenemann M2, Hansen IA1, Xue RD3, Ettestad P4, Melman S4, Duguma D5, Debboun M5, Xu J1.

Author information: 1 Biology Department New Mexico State University Las Cruces New Mexico. 2 Department of Geography New Mexico State University Las Cruces New Mexico. 3 Anastasia Mosquito Control District St. Augustine Florida. 4 New Mexico Department of Health Santa Fe New Mexico. 5 Harris County Public Health Mosquito and Vector Control Division Houston Texas.

 

Abstract

The mosquitoes Aedes aegypti (L.) and Ae. albopictus Skuse are the major vectors of dengue, Zika, yellow fever, and chikungunya viruses worldwide. Wolbachia, an endosymbiotic bacterium present in many insects, is being utilized in novel vector control strategies to manipulate mosquito life history and vector competence to curb virus transmission. Earlier studies have found that Wolbachia is commonly detected in Ae. albopictus but rarely detected in Ae. aegypti. In this study, we used a two-step PCR assay to detect Wolbachia in wild-collected samples of Ae. aegypti. The PCR products were sequenced to validate amplicons and identify Wolbachia strains. A loop-mediated isothermal amplification (LAMP) assay was developed and used for detecting Wolbachia in selected mosquito specimens as well. We found Wolbachiain 85/148 (57.4%) wild Ae. aegypti specimens from various cities in New Mexico, and in 2/46 (4.3%) from St. Augustine, Florida. Wolbachiawas not detected in 94 samples of Ae. aegypti from Deer Park, Harris County, Texas. Wolbachia detected in Ae. aegypti from both New Mexico and Florida was the wAlbB strain of Wolbachia pipientis. A Wolbachia-positive colony of Ae. aegypti was established from pupae collected in Las Cruces, New Mexico, in 2018. The infected females of this strain transmitted Wolbachia to their progeny when crossed with males of Rockefeller strain of Ae. aegypti, which does not carry Wolbachia. In contrast, none of the progeny of Las Cruces males mated to Rockefeller females were infected with Wolbachia.

KEYWORDS: Aedes aegypti; Aedes albopictus; Florida; New Mexico; Texas; Wolbachia; wAlbB

PMID: 31161026 PMCID: PMC6540660 DOI: 10.1002/ece3.5198

Keywords: Arbovirus; Mosquitoes; Aedes aegypti; Aedes albopictus; New Mexico; Florida; USA.

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#SARI Associated with Human #Metapneumovirus in Nursing Home, #NM, #USA (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 25, Number 2—February 2019 / Research Letter

Severe Respiratory Illness Associated with Human Metapneumovirus in Nursing Home, New Mexico, USA

Sandra A. Peña1, Sarah Shrum Davis, Xiaoyan Lu, Senthil Kumar K. Sakthivel, Teresa C.T. Peret, Erica Billig Rose, Chad Smelser, Eileen Schneider  , Nimalie D. Stone, and John Watson

Author affiliations: New Mexico Department of Health, Santa Fe, New Mexico, USA (S.A. Peña, S. Shrum Davis, C. Smelser); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (X. Lu, S.K.K. Sakthivel, T.C.T. Peret, E. Billig Rose, E. Schneider, N.D. Stone, J. Watson)

 

Abstract

Human metapneumovirus is an emerging pathogen that causes upper and lower respiratory illness. Nursing home outbreaks of infection with this virus can cause severe illness and lead to poor patient outcomes. We report an outbreak investigation in a nursing home during 2018 and infection control guidelines to assist in disease control.

Keywords: Metapneumovirus; SARI; USA; New Mexico; Nosocomial outbreaks.

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Small-Scale Die-Offs in #Woodrats Support Long-Term Maintenance of #Plague in the #US Southwest (Vector Borne Zoo Dis, abstract)

[Source: Vector Borne and Zoonotic Diseases, full page: (LINK). Abstract, edited.]

Vector-Borne and Zoonotic Diseases

Small-Scale Die-Offs in Woodrats Support Long-Term Maintenance of Plague in the U.S. Southwest

To cite this article:

Kosoy Michael, Reynolds Pamela, Bai Ying, Sheff Kelly, Enscore Russell E., Montenieri John, Ettestad Paul, and Gage Kenneth. Vector-Borne and Zoonotic Diseases. August 2017, ahead of print. https://doi.org/10.1089/vbz.2017.2142

Online Ahead of Print: August 9, 2017

Author information: Michael Kosoy,1 Pamela Reynolds,2 Ying Bai,1 Kelly Sheff,1 Russell E. Enscore,1 John Montenieri,1 Paul Ettestad,2 and Kenneth Gage1

1Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado. 2New Mexico Department of Health, Santa Fe, New Mexico.

© Michael Kosoy et al. 2017; Published by Mary Ann Liebert, Inc. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Address correspondence to: Michael Kosoy, Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, E-mail: mck3@cdc.gov

 

ABSTRACT

Our longitudinal study of plague dynamics was conducted in north-central New Mexico to identify which species in the community were infected with plague, to determine the spatial and temporal patterns of the dynamics of plague epizootics, and to describe the dynamics of Yersinia pestis infection within individual hosts. A total of 3156 fleas collected from 535 small mammals of 8 species were tested for Y. pestis DNA. Nine fleas collected from six southern plains woodrats (Neotoma micropus) and from one rock squirrel (Otospermophilus variegatus) were positive for the pla gene of Y. pestis. None of 127 fleas collected from 17 woodrat nests was positive. Hemagglutinating antibodies to the Y. pestis-specific F1 antigen were detected in 11 rodents of 6 species. All parts of the investigated area were subjected to local disappearance of woodrats. Despite the active die-offs, some woodrats always were present within the relatively limited endemic territory and apparently were never exposed to plague. Our observations suggest that small-scale die-offs in woodrats can support maintenance of plague in the active U.S. Southwestern focus.

Keywords: Plague; Yersinia Pestis; USA; New Mexico; Wildlife.

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