[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]
Identification of naturally processed mumps virus epitopes by mass spectrometry: Confirmation of multiple CD8+ T cell responses in mumps patients
Jelle de Wit, Maarten E Emmelot, Hugo Meiring, Jacqueline A M van Gaans-van den Brink, Cécile A C M van Els, Patricia Kaaijk
The Journal of Infectious Diseases, jiz480, https://doi.org/10.1093/infdis/jiz480
Published: 27 September 2019
The re-emergence of mumps among vaccinated young adults has become a global issue. Besides waning of antibody responses, suboptimal induction of T cell responses may reduce protection. Recently, we observed a dominant polyfunctional CD8+ T cell response after natural mumps virus (MuV) infection that was not present after vaccination. Unraveling the MuV epitope-repertoire can provide insight in the specificity, functionality, and breadth of the T cell response against MuV.
Peptides were eluted from HLA class I molecules of MuV-infected cells and characterized by advanced mass spectrometry. Selected identified MuV peptides were tested for in vitro and ex vivo immunogenicity.
Here we identified a broad landscape of 83 CD8+ T cell epitopes of MuV, of which 41 were confirmed based on synthetic peptide standards. For six epitopes we showed induction of an HLA-A*02-restriced CD8+ T cell response. Moreover, robust T cell responses against five selected MuV epitopes could be detected in all tested mumps patients using peptide/HLA-A*02:01 dextramers.
The identified CD8+ T cell epitopes will help to further characterize MuV-specific T cell immunity following natural MuV infection or vaccination. These MuV epitopes may provide clues for a better understanding of, and possibly for preventing, mumps vaccine failure.
MMR, immunopeptidome, T cell immunity, mumps infection, MHC class I
Issue Section: Major Article
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© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.
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Keywords: Mumps; Vaccines.