[Source: Journal of Antimicrobial Chemotherapy, full page: (LINK). Summary, edited.]
Detection of a novel mcr-5.4 gene variant in hospital tap water by shotgun metagenomic sequencing
Giuseppe Fleres, Natacha Couto, Leonard Schuele, Monika A Chlebowicz,Catarina I Mendes, Luc W M van der Sluis, John W A Rossen, Alex W Friedrich,Silvia García-Cobos
Journal of Antimicrobial Chemotherapy, dkz363, https://doi.org/10.1093/jac/dkz363
Published: 23 August 2019
Issue Section: Research letter
Colistin is considered a last-resort antibiotic for treating serious infections caused by MDR Gram-negative bacteria. The efficacy of this antibiotic is challenged by the emergence and global spread of mobile colistin resistance (mcr) determinants, which threaten human, animal and environmental health. The first mobile colistin resistance gene (mcr-1) was reported in 2015 and since then up to eight different variants have been described.1 In 2017, Borowiak et al.2 described a new transposon-associated phosphoethanolamine transferase mediating colistin resistance, named mcr-5, in d-tartrate-fermenting Salmonella enterica subsp. enterica serovar Paratyphi B isolated from poultry. The mcr-5.3 variant has been recently reported in Stenotrophomonas spp. from sewage water.3 Here we report for the first time (to the best of our knowledge) the detection of an mcr-5 gene in a hospital water environment using short-read metagenomic sequencing (SRMseq) and subsequent characterization using long-read metagenomic sequencing (LRMseq) to reveal its genetic environment.
We would like to thank Erwin C. Raangs for technical assistance.
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement 713660 (MSCA-COFUND-2015-DP ‘Pronkjewail’), which includes in-kind contributions by commercial partners. None of the commercial partners had any influence on interpretation of reviewed data and conclusions drawn, or on drafting of the manuscript. This work was partly supported by the INTERREG VA (202085)-funded project EurHealth-1Health, part of a Dutch–German cross-border network supported by the European Commission, the Dutch Ministry of Health, Welfare and Sport (VWS), the Ministry of Economy, Innovation, Digitalization and Energy of the German Federal State of North Rhine-Westphalia and the German Federal State of Lower Saxony.
None to declare.
Keywords: Antibiotics; Drugs Resistance; Colistin; MCR5; Germany.