Pathogenic #Leptospira Species in Insectivorous #Bats, #China, 2015 (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 24, Number 6—June 2018 / Dispatch

Pathogenic Leptospira Species in Insectivorous Bats, China, 2015

Hui-Ju Han, Hong-Ling Wen, Jian-Wei Liu, Xiang-Rong Qin, Min Zhao, Li-Jun Wang, Li-Mei Luo, Chuan-Min Zhou, Ye-Lei Zhu, Rui Qi, Wen-Qian Li, Hao Yu, and Xue-Jie Yu

Author affiliations: Wuhan University, Wuhan, China (H.-J. Han, J.-W. Liu, X.-R. Qin, M. Zhao, L.-J. Wang, C.-M. Zhou, R. Qi, X.-J. Yu); Shandong University, Jinan, China (H.-L. Wen, Y.-L. Zhu, W.-Q. Li); Shandong Center for Disease Control and Prevention, Jinan (L.-M. Luo); Fudan University, Shanghai, China (H. Yu)

 

Abstract

PCR amplification of the rrs2 gene indicated that 50% (62/124) of insectivorous bats from eastern China were infected with Leptospira borgpetersenii, L. kirschneri, and several potentially new Leptospira species. Multilocus sequence typing defined 3 novel sequence types in L. kirschneri, suggesting that bats are important carriers of Leptospira.

Keywords: Leptospira spp.; Bats; China.

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#NYC House #Mice (Mus musculus) as Potential #Reservoirs for Pathogenic #Bacteria and #Antimicrobial Resistance Determinants (mBio, abstract)

[Source: mBio, full page: (LINK). Abstract, edited.]

New York City House Mice (Mus musculus) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants

Simon H. Williams a,  Xiaoyu Che a,  Ashley Paulick b, Cheng Guo a, Bohyun Lee a, Dorothy Muller a, Anne-Catrin Uhlemann c, Franklin D. Lowy c, Robert M. Corrigan d, W. Ian Lipkin a

a Center for Infection and Immunity, Columbia University, New York, New York, USA; b Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia, USA; c Division of Infectious Diseases, Department of Medicine, Columbia University, New York, New York, USA; d RMC Pest Management Consulting, Briarcliff Manor, New York, USA

Claire M. Fraser, Editor

Author Affiliations: University of Maryland, School of Medicine

Address correspondence to W. Ian Lipkin, wil2001@cumc.columbia.edu.

 

ABSTRACT

House mice (Mus musculus) thrive in large urban centers worldwide. Nonetheless, little is known about the role that they may play in contributing to environmental contamination with potentially pathogenic bacteria. Here, we describe the fecal microbiome of house mice with emphasis on detection of pathogenic bacteria and antimicrobial resistance genes by molecular methods. Four hundred sixteen mice were collected from predominantly residential buildings in seven sites across New York City over a period of 13 months. 16S rRNA sequencing identified Bacteroidetes as dominant and revealed high levels of Proteobacteria. A targeted PCR screen of 11 bacteria, as indicated by 16S rRNA analyses, found that mice are carriers of several gastrointestinal disease-causing agents, including Shigella, Salmonella, Clostridium difficile, and diarrheagenic Escherichia coli. Furthermore, genes mediating antimicrobial resistance to fluoroquinolones (qnrB) and β-lactam drugs (blaSHV and blaACT/MIR) were widely distributed. Culture and molecular strain typing of C. difficile revealed that mice harbor ribotypes associated with human disease, and screening of kidney samples demonstrated genetic evidence of pathogenic Leptospira species. In concert, these findings support the need for further research into the role of house mice as potential reservoirs for human pathogens and antimicrobial resistance in the built environment.

 

IMPORTANCE

Mice are commensal pests often found in close proximity to humans, especially in urban centers. We surveyed mice from seven sites across New York City and found multiple pathogenic bacteria associated with febrile and gastrointestinal disease as well as an array of antimicrobial resistance genes.

KEYWORDS: antimicrobial resistance –  bacteriome –  mice –  New York City

 

FOOTNOTES

Citation Williams SH, Che X, Paulick A, Guo C, Lee B, Muller D, Uhlemann A-C, Lowy FD, Corrigan RM, Lipkin WI. 2018. New York City house mice (Mus musculus) as potential reservoirs for pathogenic bacteria and antimicrobial resistance determinants. mBio 9:e00624-18. https://doi.org/10.1128/mBio.00624-18.

For a companion article on this topic, see https://doi.org/10.1128/mBio.01354-17.

Received 19 March 2018  – Accepted 22 March 2018  – Published 17 April 2018

Copyright © 2018 Williams et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Keywords: USA; New York City; House Mice; Antibiotics; Drugs Resistance; Clostridium difficile; Leptospira spp.; Shigella spp.; Salmonella spp.; E. Coli.

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Exposure to #Rats and Rat-Associated #Leptospira and #Bartonella Species Among People Who Use #Drugs in an Impoverished, Inner-City Neighborhood of #Vancouver, Canada (Vector Borne Zoo Dis., abstract)

[Source: Vector-Borne and Zoonotic Diseases, full page: (LINK). Abstract, edited.]

Vector-Borne and Zoonotic Diseases

Exposure to Rats and Rat-Associated Leptospira and Bartonella Species Among People Who Use Drugs in an Impoverished, Inner-City Neighborhood of Vancouver, Canada

To cite this article: McVea David A., Himsworth Chelsea G., Patrick David M., Lindsay L. Robbin, Kosoy Michael, and Kerr Thomas. Vector-Borne and Zoonotic Diseases. January 2018, ahead of print. https://doi.org/10.1089/vbz.2017.2179

Online Ahead of Print: January 3, 2018

Author information: David A. McVea,1,* Chelsea G. Himsworth,1,2,* David M. Patrick,1 L. Robbin Lindsay,3 Michael Kosoy,4 and Thomas Kerr5,6

1School of Population and Public Health, University of British Columbia, Vancouver, Canada. 2BC Node, Canadian Wildlife Health Cooperative, Abbotsford, Canada. 3National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada. 4Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado. 5British Columbia Centre for Excellence in HIV/AIDS, Providence Health Care, Vancouver, Canada. 6Department of Medicine, University of British Columbia, Vancouver, Canada.

*These authors contributed equally to this work.

Address correspondence to: Chelsea G. Himsworth, School of Population and Public Health, University of British ColumbiaVancouver V6T 1Z3, British Columbia, Canada
E-mail: chelsea.himsworth@gov.bc.ca

 

ABSTRACT

Rat infestations are common, particularly in impoverished, inner-city neighborhoods. However, there has been little research into the nature and consequences of rat exposure in these neighborhoods, particularly in Canada. In this study, we sought to characterize exposure to rats and rat-associated Leptospira interrogans and Bartonella tribocorum, as well as risk factors associated with exposure, in residents (n = 202) of the Downtown Eastside (DTES) neighborhood of Vancouver, Canada. There was no evidence of exposure to rat-associated L. interrogans but 6/202 (3.0%) of participants were exposed to B. tribocorum, which is known to be circulating among DTES rats. We also found that frequent and close rat exposure was common among DTES residents, and that this exposure was particularly associated with injection drug use and outdoor income-generating activities (e.g., drug dealing). These risk factors may be good targets for interventions geared toward effectively reducing rat exposure.

Keywords: Illicit Drugs Users; Bartonella spp.; Leptospira spp.

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