[Source: Eurosurveillance, full page: (LINK). Abstract, edited.]
Genetic and antigenic characterisation of influenza A(H3N2) viruses isolated in Yokohama during the 2016/17 and 2017/18 influenza seasons
Chiharu Kawakami1, Seiya Yamayoshi2, Miki Akimoto3, Kazuya Nakamura3, Hideka Miura3, Seiichiro Fujisaki3, David J. Pattinson4,Kohei Shimizu1, Hiroki Ozawa1, Tomoko Momoki1, Miwako Saikusa1, Atsuhiro Yasuhara2, Shuzo Usuku1, Ichiro Okubo1, Takahiro Toyozawa5,Shigeo Sugita6, Derek J. Smith4, Shinji Watanabe3, Yoshihiro Kawaoka2,7,8
Affiliations: 1 Yokohama City Institute of Public Health, Yokohama, Japan; 2 Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan; 3 Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan; 4 Center for Pathogen Evolution, University of Cambridge, Cambridge, UK; 5 Yokohama City Public Health Center, Yokohama, Japan; 6 Equine Research Institute, Japan Racing Association, Tochigi, Japan; 7 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, USA; 8 Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Correspondence: Yoshihiro Kawaoka
Citation style for this article: Kawakami Chiharu, Yamayoshi Seiya, Akimoto Miki, Nakamura Kazuya, Miura Hideka, Fujisaki Seiichiro, Pattinson David J., Shimizu Kohei,Ozawa Hiroki, Momoki Tomoko, Saikusa Miwako, Yasuhara Atsuhiro, Usuku Shuzo, Okubo Ichiro, Toyozawa Takahiro, Sugita Shigeo, Smith Derek J., Watanabe Shinji,Kawaoka Yoshihiro. Genetic and antigenic characterisation of influenza A(H3N2) viruses isolated in Yokohama during the 2016/17 and 2017/18 influenza seasons. Euro Surveill. 2019;24(6):pii=1800467. https://doi.org/10.2807/1560-7917.ES.2019.24.6.1800467
Received: 22 Aug 2018; Accepted: 25 Nov 2018
Influenza A(H3N2) virus rapidly evolves to evade human immune responses, resulting in changes in the antigenicity of haemagglutinin (HA). Therefore, continuous genetic and antigenic analyses of A(H3N2) virus are necessary to detect antigenic mutants as quickly as possible.
We attempted to phylogenetically and antigenically capture the epidemic trend of A(H3N2) virus infection in Yokohama, Japan during the 2016/17 and 2017/18 influenza seasons.
We determined the HA sequences of A(H3N2) viruses detected in Yokohama, Japan during the 2016/17 and 2017/18 influenza seasons to identify amino acid substitutions and the loss or gain of potential N-glycosylation sites in HA, both of which potentially affect the antigenicity of HA. We also examined the antigenicity of isolates using ferret antisera obtained from experimentally infected ferrets.
Influenza A(H3N2) viruses belonging to six clades (clades 3C.2A1, 3C.2A1a, 3C.2A1b, 3C.2A2, 3C.2A3 and 3C.2A4) were detected during the 2016/17 influenza season, whereas viruses belonging to two clades (clades 3C.2A1b and 3C.2A2) dominated during the 2017/18 influenza season. The isolates in clades 3C.2A1a and 3C.2A3 lost one N-linked glycosylation site in HA relative to other clades. Antigenic analysis revealed antigenic differences among clades, especially clade 3C.2A2 and 3C.2A4 viruses, which showed distinct antigenic differences from each other and from other clades in the antigenic map.
Multiple clades, some of which differed antigenically from others, co-circulated in Yokohama, Japan during the 2016/17 and 2017/18 influenza seasons.
© This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords: Seasonal Influenza; H3N2; Japan.