#Epidemiological and #clinical characteristics of 671 #COVID19 patients in #Henan Province, #China (Int J Epidemiol., abstract)

[Source: International Journal of Epidemiology, full page: (LINK). Abstract, edited.]

Epidemiological and clinical characteristics of 671 COVID-19 patients in Henan Province, China

Yifei Nie, Jitian Li, Xueyong Huang, Wanshen Guo, Xiaobai Zhang, Yan Ma, Haifeng Wang, Muge Qi, Xiaoyan Tang, Xiaojing Shen, Xiaofeng Dai

International Journal of Epidemiology, dyaa081, https://doi.org/10.1093/ije/dyaa081

Published: 26 June 2020




Despite many reports on the characteristics of coronavirus disease 2019 (COVID-19) in Wuhan, China, relatively little is known about the transmission features of COVID-19 outside Wuhan, especially at the provincial level.


We collected epidemiological, demographic, clinical, laboratory, radiological and occupation information, along with contact history, of 671 patients with laboratory-confirmed COVID-19 reported from January 23 to February 5, 2020, in Henan province, China. We described characteristics of these cases, compared the diagnostic accuracy and features of blood testing, computed tomography (CT) scans and X-rays, and analysed SARS-CoV-2 transmission sources and patients’ occupations in Henan province.


The mean age of patients in this case series was 43 years, 56.2% were male and 22.4% had coexisting medical disorders. The death rate was 0.3%. Fourteen patients did not show any symptoms. Lymphocyte percentage was associated with disease severity (χ2 = 6.71, P = 0.035) but had a large variation in each sample group. The mean time from illness onset to diagnosis was 5.6 days. A total of 330 patients had ever lived in or visited Wuhan, 150 had contact with confirmed cases, 323 had been to a hospital and 119 had been to a wet market. There were 33 patients who did not have a traceable transmission source, with 21.2% of these being farmers and 15.2% being workmen.


Lymphocyte percentage was a sign of severe COVID-19 in general but was not a good diagnostic index. Longer time from illness onset to diagnosis was associated with higher COVID-19 severity, older age, higher likelihood of having coexisting cardiovascular diseases including hypertension, and being male. Farming was found to be a high-risk occupation in Henan province, China.

SARS-CoV-2, COVID-19, Henan province

Issue Section: Original Article


Key Messages

  • This study presents the epidemiological and clinical characteristics of a patient cohort of 671 confirmed COVID-19 cases in Henan province, the largest province in terms of population size and ranked as the third most severely affected province in China.
  • To the best of our knowledge, our study is the largest report of COVID-19 clinical characteristics in China outside Wuhan at the provincial level that offers direct evidence on SARS-CoV-2 transmission manifestations.
  • Insights gained from this study could guide clinical practice on COVID-19 diagnosis and pathogen transmission control at both governmental and individual levels.

Keywords: SARS-CoV-2; COVID-19; Henan; China.


Distribution of #Avian #Influenza A Viruses in #Poultry-Related #Environment and Its Association with #Human #Infection in #Henan, 2016 to 2017 (Biomed Environ Sci., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Biomed Environ Sci. 2019 Nov;32(11):797-803. doi: 10.3967/bes2019.101.

Distribution of Avian Influenza A Viruses in Poultry-Related Environment and Its Association with Human Infection in Henan, 2016 to 2017.

Ma HX1, Wang RL2, Nie YF2, Su J2, Li DX1, Li Y1, DU YH1, Wei HY1, Li XL1, Wang Z2, Xu BL1, Huang XY1.

Author information: 1 Henan Centers for Disease Control and Prevention, Zhengzhou 450016, Henan, China; Key Laboratory of Pathogenic Microorganisms of Henan Province, Zhengzhou 450016, Henan, China. 2 Henan Centers for Disease Control and Prevention, Zhengzhou 450016, Henan, China.




To survey avian influenza A viruses (AIVs) in the environment and explore the reasons for the surge in human H7N9 cases.


A total of 1,045 samples were collected from routine surveillance on poultry-related environments and 307 samples from human H7N9 cases-exposed environments in Henan from 2016 to 2017. The nucleic acids of influenza A (Flu A), H5, H7, and H9 subtypes were detected by real-time polymerase chain reaction.


A total of 27 H7N9 cases were confirmed in Henan from 2016 to 2017, 24 had a history of live poultry exposure, and 15 had H7N9 virus detected in the related live poultry markets (LPMs). About 96% (264/275) Flu A positive-environmental samples were from LPMs. H9 was the main AIV subtype (10.05%) from routine surveillance sites with only 1 H7-positive sample, whereas 21.17% samples were H7-positive in H7N9 cases-exposed environments. Samples from H7N9 cases-exposed LPMs (47.56%) had much higher AIVs positive rates than those from routine surveillance sites (12.34%). The H7+H9 combination of mixed infection was 78.18% (43/55) of H7-positive samples and 41.34% (43/104) of H9-positive samples.


The contamination status of AIVs in poultry-related environments is closely associated with the incidence of human infection caused by AIVs. Therefore, systematic surveillance of AIVs in LPMs in China is essential for the detection of novel reassortant viruses and their potential for interspecies transmission.

Copyright © 2019 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

KEYWORDS: Avian influenza virus; Exposure environments; Human H7N9 cases; Live poultry market; Routine surveillance

PMID: 31910937 DOI: 10.3967/bes2019.101

Keywords: Avian Influenza; H7N9; H9N2; Poultry; Human; China; Henan.


#Seroprevalance of #antibodies specific for #SFTS virus and the discovery of #asymptomatic #infections in #Henan Province, #China (PLOS Negl Trop Dis., abstract)

[Source: PLOS Neglected Tropical Diseases, full page: (LINK). Abstract, edited.]


Seroprevalance of antibodies specific for severe fever with thrombocytopenia syndrome virus and the discovery of asymptomatic infections in Henan Province, China

Yanhua Du , Ningning Cheng , Yi Li, Haifeng Wang, Aiguo You, Jia Su, Yifei Nie, Hongxia Ma, Bianli Xu , Xueyong Huang


Published: November 25, 2019 / DOI: https://doi.org/10.1371/journal.pntd.0007242 / This is an uncorrected proof.




Severe fever with thrombocytopenia syndrome (SFTS) is a severe emerging disease caused by SFTS virus (SFTSV), and the geographical distribution of SFTS has been increasing throughout China in recent years. To assess SFTSV-specific antibody seroprevalence, a cross-sectional study was conducted for healthy people in high SFTS endemic areas of Henan province in 2016.


This study used a stratified random sampling method to select 14 natural villages as the investigation sites. From April to May 2016, participants completed a questionnaire survey and serum samples were collected. All serum samples were subjected to ELISA to detect SFTSV-specific IgM and IgG. All IgM-positive samples were further tested by real-time RT-PCR, and isolation of virus from serum was attempted. Any participant who was IgM-positive was followed up with a month later to confirm health status.


In total, 1463 healthy people participated in this study. The average seropositive rates for SFTSV-specific IgG and IgM were 10.46% (153/1463) and 0.82% (12/1463), respectively. IgM was detected in 12 individuals, and SFTSV RNA was detected in six of them. Virus was isolated from five of the six SFTSV RNA-positive individuals, and phylogenetic analyses revealed that all five isolates belonged to SFTSV group A. No IgM-positive participants exhibited any symptoms or other signs of illness at the one-month follow up.


This study identified a relatively high incidence of SFTSV-specific antibody seropositivity in healthy people in Xinyang city. Moreover, our data provide the first evidence for asymptomatic SFTSV infections, which may have significant implications for SFTS outbreak control.


Author summary

Severe fever with thrombocytopenia syndrome (SFTS) is a severe emerging infectious disease caused by SFTS virus (SFTSV) that was first discovered in rural areas of China. Henan province has had the largest number of SFTS cases in China every year since the disease was discovered, however, seropositivity for SFTSV-specific antibodies in healthy people in this region is still not clear. To address this issue, a cross-sectional survey was performed in high endemic areas from April to May 2016. The results showed that SFTSV seroprevalence was relatively high and possibly increasing. Notably, SFTSV RNA, as well as virus itself, was isolated from specimens obtained from healthy people. This study confirmed there are asymptomatic SFTSV infections in humans, and it is the first to report SFTSV isolation from healthy people.


Citation: Du Y, Cheng N, Li Y, Wang H, You A, Su J, et al. (2019) Seroprevalance of antibodies specific for severe fever with thrombocytopenia syndrome virus and the discovery of asymptomatic infections in Henan Province, China. PLoS Negl Trop Dis 13(11): e0007242. https://doi.org/10.1371/journal.pntd.0007242

Editor: Abdallah M. Samy, Faculty of Science, Ain Shams University (ASU), EGYPT

Received: February 10, 2019; Accepted: October 4, 2019; Published: November 25, 2019

Copyright: © 2019 Du et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the manuscript and its Supporting Information files.

Funding: H.X.Y received grants from the National Natural Science Foundation of China (NO 81573204 https://isisn.nsfc.gov.cn/egrantweb/) and Henan provincial medical science and technology program (grant no.2018010029) .X.B.L. recieved a grant from the National Natural Science Foundation of China (NO 81773500 https://isisn.nsfc.gov.cn/egrantweb/).D.Y.H received a grant from Henan provincial medical science and technology program (grant no.2018020510). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Keywords: Serology; Seroprevalence; SFTS; Henan; China.


Molecular #surveillance of #drug #resistance of #Plasmodium falciparum isolates imported from #Angola in #Henan Province, #China (Antimicrob Agents Chemother., abstract)

[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]

Molecular surveillance of drug resistance of Plasmodium falciparum isolates imported from Angola in Henan Province, China

Ruimin Zhou, Chengyun Yang, Suhua Li, Yuling Zhao, Ying Liu, Dan Qian, Hao Wang, Deling Lu, Hongwei Zhang, Fang Huang

DOI: 10.1128/AAC.00552-19



Angola was the main origin country for the imported malaria in Henan Province, China. The antimalarial drug resistance has posed a threat to the control and elimination of malaria. Several molecular markers were confirmed to be associated with the antimalarial drug resistance such as pfcrt, pfmdr1, pfdhfr, pfdhps and K13. This study will evaluate the drug resistance of the 180 imported Plasmodium falciparum isolates from Angola via nested PCR by Sanger sequencing. The prevalence of pfcrt C72V73M74N75K76, pfmdr1 N86Y184S1034N1042D1246, pfdhfr A16N51C59S108D139I164 and pfdhps S436A437A476K540A581 was 69.4%, 59.9%, 1.3% and 6.3%, respectively. Three nonsynonymous (A578S, M579I and Q613E) and one synonymous (R471R) mutation of K13 were found, the prevalence of which was 2.5% and 1.3%, respectively. The single nucleotide polymorphisms (SNPs) in pfcrt, pfmdr1, pfdhfr and pfdhps were generally shown as multiple mutations. The mutant prevalence of pfcrt reduced gradually, but pfdhfr and pfdhps still showed high mutant prevalence, while pfmdr1 was relatively low. The mutation of K13 gene was rare. Molecular surveillance of artemisinin-resistance (ART-resistance) will be as a tool to evaluate the real time efficacy of the artemisinin-based combination therapies (ACTs) and the ART-resistance situation as well.

Copyright © 2019 American Society for Microbiology. All Rights Reserved.

Keywords: Plasmodium falciparum; Malaria; Drugs Resistance; Artemisin; Angola; Henan; China.