#MERS #coronavirus infection in non-camelid domestic #mammals (Emerg Microbes Infect., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Emerg Microbes Infect. 2019;8(1):103-108. doi: 10.1080/22221751.2018.1560235.

Middle East respiratory syndrome coronavirus infection in non-camelid domestic mammals.

Kandeil A1, Gomaa M1, Shehata M1, El-Taweel A1, Kayed AE1, Abiadh A2, Jrijer J2, Moatasim Y1, Kutkat O1, Bagato O1, Mahmoud S1, Mostafa A1,3, El-Shesheny R1,4, Perera RA5, Ko RL5, Hassan N6, Elsokary B6, Allal L7, Saad A7, Sobhy H7, McKenzie PP4, Webby RJ4, Peiris M5, Ali MA1, Kayali G8,9.

Author information: 1 a Center of Scientific Excellence for Influenza Virus , National Research Centre , Giza , Egypt. 2 b Nature Link , Sfax , Tunisia. 3 c Institute of Medical Virology , Justus Liebig University Giessen , Giessen , Germany. 4 d St. Jude Children’s Research Hospital , Memphis , TN , USA. 5 e School of Public Health , University of Hong Kong , Sandy Bay , Hong Kong. 6 f General Organizations of Veterinary Services , Ministry of Agriculture and Land Reclamation , Giza , Egypt. 7 g Food and Agriculture Organization of the United Nations , Emergency Center for Transboundary Animal Diseases , Giza , Egypt. 8 h Human Link , Baabda , Lebanon. 9 i University of Texas Health Sciences Center , Houston , TX , USA.

 

Abstract

Dromedary camels are natural host of the Middle East respiratory syndrome coronavirus (MERS-CoV). However, there are limited studies of MERS-CoV infection of other domestic mammals exposed to infected dromedaries. We expanded our surveillance among camels in Egypt, Tunisia, and Senegal to include other domestic mammalian species in contact with infected camels. A total of 820 sera and 823 nasal swabs from cattle, sheep, goats, donkeys, buffaloes, mules, and horses were collected. Swabs were tested using RT-PCR and virus RNA-positive samples were genetically sequenced and phylogenetically analysed. Sera were screened using virus microneutralization tests and positive sera (where available) were confirmed using plaque reduction neutralization tests (PRNT). We detected 90% PRNT confirmed MERS-CoV antibody in 35 (55.6%) of 63 sera from sheep collected from Senegal, two sheep (1.8%) of 114 in Tunisia and a goat (0.9%) of 107 in Egypt, with titres ranging from 1:80 to ≥1:320. We detected MERS-CoV RNA in swabs from three sheep (1.2%) of 254 and five goats (4.1%) of 121 from Egypt and Senegal, as well as one cow (1.9%) of 53 and three donkeys (7.1%) of 42 from Egypt. Partial sequences of the RT-PCR amplicons confirmed specificity of the results. This study showed that domestic livestock in contact with MERS-CoV infected camels may be at risk of infection. We recommend expanding current MERS-CoV surveillance in animals to include other livestock in close contact with dromedary camels. The segregation of camels from other livestock in farms and live animal markets may need to be considered.

KEYWORDS: Egypt; MERS-CoV; Senegal; Tunisia; serology; sheep; surveillance

PMID: 30866764 DOI: 10.1080/22221751.2018.1560235

Keywords: MERS-CoV; Sheeps; Goats; Senegal; Tusinia; Egypt.

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The #EU #summary #report on #surveillance for the presence of transmissible spongiform #encephalopathies (#TSEs) in 2017 (EFSA, abstract)

[Source: European Food Safety Authority (EFSA), full page: (LINK). Abstract, edited.]

Scientific Report  / Open Access

The European Union summary report on surveillance for the presence of transmissible spongiform encephalopathies (TSEs) in 2017

European Food Safety Authority (EFSA) / First published: 28 November 2018 / DOI:  https://doi.org/10.2903/j.efsa.2018.5492

Correspondence: zoonoses@efsa.europa.eu

Requestor: European Commission

Question number: EFSA‐Q‐2017‐00753

Acknowledgements: EFSA wishes to thank for the support provided to this scientific output to the EFSA staff members: Yves Van der Stede, Angel Ortiz Pelaez, Valentina Rizzi, Pietro Stella and Frank Boelaert, and to the EFSA contractor: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta (Unit BEAR – Biostatistica Epidemiologia e Analisi del Rischio and staff: Giuseppe Ru, Francesco Ingravalle, Cristina Bona, Rosanna Desiato, Cristiana Maurella and Eleonora Aiassa).

Approved: 6 November 2018

 

Abstract

This report presents the results of surveillance on transmissible spongiform encephalopathies (TSEs) in bovine animals, sheep, goats, cervids and other animal species, as well as genotyping in sheep, carried out in 2017 in the European Union (EU) according to Regulation (EC) 999/2001, and in Iceland, Norway and Switzerland. In total, 1,312,714 cattle were tested by the 28 EU Member States (MSs) which is a decrease of 3% compared with 2016; 18,526 were tested by the three non‐MSs. For the first time since bovine spongiform encephalopathy (BSE) has been reported, no cases of classical BSE were reported in 2017. Six atypical BSE cases were reported by three different MSs: Spain 1 H‐BSE/2 L‐BSE; France 1 H‐BSE/1 L‐BSE; and Ireland 1 L‐BSE. Over the year, 314,547 sheep and 117,268 goats were tested in the EU. In sheep, 933 cases of scrapie were reported: 839 classical and unknown (145 index cases) by eight MSs and 94 atypical (89 index cases) by 13 MSs. Fourteen ovine scrapie cases were reported by Iceland and Norway. Of all classical scrapie cases, 98.2% occurred in sheep with genotypes of susceptible groups. The genotyping of a random sample in 21 MSs showed that 26.5% of the genotyped sheep carried genotypes of the susceptible groups. In goats 567 cases of scrapie were reported: 558 classical (42 index cases) by seven MSs and nine atypical (seven index cases) by five MSs. In total, 3,585 cervids were tested for TSE by ten MSs, mostly by Romania. All results were negative. Eleven cases of chronic wasting disease (CWD) cases were reported in cervids by Norway: nine wild reindeer, one moose and, for the first time ever, one red deer. In total, 185 animals from five species other than cattle, small ruminants and cervids were tested by three MSs, with negative results.

Keywords: Prions; TSE; Mad Cow; Scrapie; Chronic Wasting Disease; Cattle; Cervids; Sheeps; EU.

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#Influenza D Virus in #Animal #Species in #Guangdong Province, Southern #China (@CDC_EIDjournal, abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 23, Number 8—August 2017 / Dispatch

Influenza D Virus in Animal Species in Guangdong Province, Southern China

Shao-Lun Zhai1, He Zhang1, Sheng-Nan Chen1, Xia Zhou, Tao Lin, Runxia Liu, Dian-Hong Lv, Xiao-Hui Wen, Wen-Kang Wei1, Dan Wang, and Feng Li

Author affiliations: South Dakota State University, Brookings, South Dakota, USA (S.-L. Zhai, S.-N. Chen, T. Lin, R. Liu, D. Wang, F. Li); Guangdong Academy of Agricultural Sciences, Guangzhou, China (S.-L. Zhai, D.-H. Lv, X.-H. Wen, W.-K. Wei); South China Agricultural University, Guangzhou (H. Zhang, X. Zhou)

 

Abstract

Molecular tests revealed influenza D viruses of D/OK lineage widely circulating in farmed animal species in Guangdong Province, southern China. In particular, we found high levels of influenza D virus infection in goats and pigs. We also detected viral RNA in serum specimens and feces of animals with certain severe diseases.

Keywords: Influenza D virus; Pigs; Goats; China; Guangdong.

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#Inoculation of #Goats, #Sheep, and #Horses with #MERS-CoV Does Not Result in Productive Viral Shedding (Viruses, abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Viruses. 2016 Aug 19;8(8). pii: E230.

Inoculation of Goats, Sheep, and Horses with MERS-CoV Does Not Result in Productive Viral Shedding.

Adney DR1, Brown VR2, Porter SM3, Bielefeldt-Ohmann H4, Hartwig AE5, Bowen RA6,7.

Author information: 1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80521, USA. danielle.adney@colostate.edu. 2Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80521, USA. vienna.brown@colostate.edu. 3Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. stephanie.porter@colostate.edu. 4School of Veterinary Science, University of Queensland, Gatton, QLD 4343, Australia. h.bielefeldtohmann1@uq.edu.au. 5Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. airn.hartwig@colostate.edu. 6Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80521, USA. Richard.Bowen@colostate.edu. 7Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. Richard.Bowen@colostate.edu.

 

Abstract

The Middle East respiratory syndrome coronavirus (MERS-CoV) was first recognized in 2012 and can cause severe disease in infected humans. Dromedary camels are the reservoir for the virus, although, other than nasal discharge, these animals do not display any overt clinical disease. Data from in vitro experiments suggest that other livestock such as sheep, goats, and horses might also contribute to viral transmission, although field data has not identified any seropositive animals. In order to understand if these animals could be infected, we challenged young goats and horses and adult sheep with MERS-CoV by intranasal inoculation. Minimal or no virus shedding was detected in all of the animals. During the four weeks following inoculation, neutralizing antibodies were detected in the young goats, but not in sheep or horses.

KEYWORDS: MERS; goat; horse; reservoir host; sheep

PMID: 27548203 DOI: 10.3390/v8080230

[PubMed – as supplied by publisher]

Keywords: Research; Abstracts; MERS-CoV; Horses; Goats; Sheeps.

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