[Source: PLoS Neglected Tropical Diseases, full page: (LINK). Abstract, edited.]
OPEN ACCESS / PEER-REVIEWED / RESEARCH ARTICLE
Chikungunya virus vector competency of Brazilian and Florida mosquito vectors
Nildimar Alves Honório , Keenan Wiggins, Daniel Cardoso Portela Câmara, Bradley Eastmond, Barry W. Alto
Published: June 7, 2018 / DOI: https://doi.org/10.1371/journal.pntd.0006521 / This is an uncorrected proof.
Chikungunya virus is a vector-borne alphavirus transmitted by the bites of infected female Ae. aegypti and Ae. albopictus. In Brazil between 2014 and 2016 almost 320 thousand autochthonous human cases were reported and in Florida numerous imported CHIKV viremic cases (> 3,800) demonstrate the potential high risk to establishment of local transmission. In the present study, we carried out a series of experiments to determine the viral dissemination and transmission rates of different Brazilian and Florida populations of Ae. aegypti and Ae. albopictus at 2, 5, and 13 days post-infection for the emergent Asian genotype of CHIKV. Our results show that all tested populations of Ae. aegypti and Ae. albopictus have a high proportion (> 0.80) of individuals with disseminated infection as early as 2 days-post exposure. We found no significant treatment effects of mosquito population origin effects on viral dissemination rates. Transmission rates had a heterogeneous pattern, with US Ae. aegypti and Brazilian Ae. albopictus having the highest proportion of individuals with successful infection (respectively 0.50 and 0.82 as early as 2 days-post infection). Model results found significant effects of population origin, population origin x species, population origin x days post-infection and population origin x species x days post infection.
Chikungunya is considered a serious mosquito-borne disease in many tropical and subtropical countries throughout the world. It is already an epidemic disease in Brazil and poses as a potential risk in Florida. It is mainly transmitted by mosquitoes Aedes aegypti and Aedes albopictus. These mosquito species are common and abundant throughout much of the year in Brazil and Florida. In this study, we determined two components of vector competence from Brazilian and Florida populations of both mosquitoes to the emergent Asian genotype of chikungunya virus: viral dissemination and transmission rates. Both Aedes populations exhibited a high proportion of disseminated infection as early as two days after ingestion of chikungunya virus infected blood. Transmission efficiency was higher in Ae. aegypti from Florida and Ae. albopictus from Brazil. Our findings suggest that mosquito-virus interactions of both Ae. aegypti and Ae. albopictus may vary by geographic population, which may impact public health measures and should be considered during outbreaks of this arboviral disease.
Citation: Honório NA, Wiggins K, Câmara DCP, Eastmond B, Alto BW (2018) Chikungunya virus vector competency of Brazilian and Florida mosquito vectors. PLoS Negl Trop Dis 12(6): e0006521. https://doi.org/10.1371/journal.pntd.0006521
Editor: Pattamaporn Kittayapong, Faculty of Science, Mahidol University, THAILAND
Received: November 20, 2017; Accepted: May 12, 2018; Published: June 7, 2018
Copyright: © 2018 Honório et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: This study was funded by the Florida Department of Agriculture and Consumer Services, contract number 021802, BWA and LPL, http://www.freshfromflorida.com/ The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Keywords: Chikungunya Fever; Mosquitoes; Aedes Aegypti; Aedes Albopictus; USA; Florida; Brazil.