[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Environ Int. 2019 Aug 29;132:105117. doi: 10.1016/j.envint.2019.105117. [Epub ahead of print]
Heavy metal pollution and co-selection for antibiotic resistance: A microbial palaeontology approach.
Dickinson AW1, Power A2, Hansen MG3, Brandt KK3, Piliposian G4, Appleby P4, O’Neill PA5, Jones RT6, Sierocinski P7, Koskella B8, Vos M9.
Author information: 1 College of Life and Environmental Science, University of Exeter, Penryn, UK; UK Centre for Astrobiology, School of Physics and Astronomy, University of Edinburgh, Edinburgh, UK. Electronic address: A.W.Dickinson@sms.ed.ac.uk. 2 Biocatalysis Centre, University of Exeter, Exeter, UK. 3 Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark. 4 Department of Mathematical Sciences, University of Liverpool, Liverpool, UK. 5 Welcome Trust Biomedical Informatics Hub, Geoffrey Pope Building, University of Exeter, Exeter, UK. 6 School of Geography, College of Life and Environmental Sciences, University of Exeter, Amory Building, Rennes Drive, Exeter, UK. 7 College of Life and Environmental Science, University of Exeter, Penryn, UK. 8 Department of Integrative Biology, University of California, Berkeley, Berkeley, CA, USA.
9 European Centre for Environment and Human Health, College of Medicine and Health, University of Exeter, Penryn, UK.
Frequent and persistent heavy metal pollution has profound effects on the composition and activity of microbial communities. Heavy metals select for metal resistance but can also co-select for resistance to antibiotics, which is a global health concern. We here document metal concentration, metal resistance and antibiotic resistance along a sediment archive from a pond in the North West of the United Kingdom covering over a century of anthropogenic pollution. We specifically focus on zinc, as it is a ubiquitous and toxic metal contaminant known to co-select for antibiotic resistance, to assess the impact of temporal variation in heavy metal pollution on microbial community diversity and to quantify the selection effects of differential heavy metal exposure on antibiotic resistance. Zinc concentration and bioavailability was found to vary over the core, likely reflecting increased industrialisation around the middle of the 20th century. Zinc concentration had a significant effect on bacterial community composition, as revealed by a positive correlation between the level of zinc tolerance in culturable bacteria and zinc concentration. The proportion of zinc resistant isolates was also positively correlated with resistance to three clinically relevant antibiotics (oxacillin, cefotaxime and trimethoprim). The abundance of the class 1 integron-integrase gene, intI1, marker for anthropogenic pollutants correlated with the prevalence of zinc- and cefotaxime resistance but not with oxacillin and trimethoprim resistance. Our microbial palaeontology approach reveals that metal-contaminated sediments from depths that pre-date the use of antibiotics were enriched in antibiotic resistant bacteria, demonstrating the pervasive effects of metal-antibiotic co-selection in the environment.
Copyright © 2019. Published by Elsevier Ltd.
KEYWORDS: Antimicrobial resistance; Co-selection; Cross-resistance; Metal pollution; Sediment archive
PMID: 31473413 DOI: 10.1016/j.envint.2019.105117
Keywords: Antibiotics; Drugs Resistance; Toxic chemicals; Environmental pollution; UK.