In vitro Evaluation of #Antifungal Drug #Combinations against #MDR #Candida auris isolates from #NY #Outbreak (Antimicrob Agents Chemother., abstract)

[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]

In vitro Evaluation of Antifungal Drug Combinations against Multidrug-resistant Candida auris isolates from New York Outbreak

Brittany O’Brien, Sudha Chaturvedi, Vishnu Chaturvedi

DOI: 10.1128/AAC.02195-19

 

ABSTRACT

Since 2016, New York hospitals and healthcare facilities have faced an unprecedented outbreak of the pathogenic yeast Candida auris. We tested over one thousand C. auris isolates from affected facilities and found high-resistance to fluconazole (MIC > 256 mg/L), and variable resistance to other antifungal drugs. Therefore, we tested if two-drug combinations are effective in vitro against multidrug-resistant C. auris. Broth micro-dilution antifungal combination plates were custom-manufactured by TREK Diagnostic System. We used 100% inhibition endpoints for the drug combination as reported earlier for the intra- and inter-laboratory agreements against Candida species. The results were derived from 12,960 readings, for fifteen C. auris isolates tested against 864 two-drug antifungal combinations for nine antifungal drugs. Flucytosine (5FC) at 1.0 mg/L potentiated the most combinations. For nine C. auris isolates resistant to amphotericin B (AMB, MIC ≥ 2.0 mg/L]), AMB/5FC (0.25/1.0 mg/L) yielded 100% inhibition. Six C. auris isolates resistant to three echinocandins (anidulafungin [AFG, MIC ≥ 4.0 mg/L], caspofungin [CAS, MIC ≥ 2.0 mg/L], and micafungin [MFG, MIC ≥ 4.0 mg/L]), were 100% inhibited by AFG/5FC and CAS/5FC (0.0078/1 mg/L), and MFG/5FC (0.12/1 mg/L). None of the combinations were effective for C. auris 18-1 and 18-13 (FLC > 256 mg/L, 5FC > 32 mg/L) except MFG/5FC (0.1/0.006 mg/L). Thirteen isolates with high voriconazole MIC (VRC, > 2 mg/L) were 100% inhibited by the VRC/5FC (0.015/1 mg/L). The simplified two-drug combination susceptibility test format would permit laboratories to provide clinicians and public health experts with additional data to manage multidrug-resistant C. auris.

Copyright © 2020 American Society for Microbiology. All Rights Reserved.

Keywords: Drugs Resistance; Candida auris; USA.

——

The Novel #Arylamidine T-2307 Demonstrates In vitro and In vivo Activity Against #Candida auris (Antimicrob Agents Chemother., abstract)

[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]

The Novel Arylamidine T-2307 Demonstrates In vitro and In vivo Activity Against Candida auris

Nathan P. Wiederhold, Laura K. Najvar, Rosie Jaramillo, Marcos Olivo, Hoja Patterson, April Connell, Yoshiko Fukuda, Junichi Mitsuyama, Gabriel Catano, Thomas F. Patterson

DOI: 10.1128/AAC.02198-19

 

ABSTRACT

The in vitro and in vivo activity of the arylamidine T-2307 against C. auris was evaluated. T-2307 demonstrated in vitro activity (MIC range ≤ 0.008 – 0.015 μg/mL at 50% inhibition; 0.125 – >4 μg/mL at 100% inhibition). Treatment with T-2307 (3 mg/kg SC once daily) also significantly improved survival (70% at 21 days post-infection) and reduced kidney fungal burden (5.06 log10 CFU/g) compared to control (0% and 7.09 log10 CFU/g; p < 0.01).

Copyright © 2019 American Society for Microbiology. All Rights Reserved.

Keywords: Candida auris; Arylamidine; Animal models.

——

Impact of #Candida auris #infection in a #neutropenic murine model (Antimicrob Agents Chemother., abstract)

[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]

Impact of Candida auris infection in a neutropenic murine model

Steven R. Torres, Amber Pichowicz, Fernando Torres-Velez, Renjie Song, Navjot Singh, Erica Lasek-Nesselquist, Magdia De Jesus

DOI: 10.1128/AAC.01625-19

 

ABSTRACT

Candida auris has become a global public health threat due to its multidrug resistance and persistence. Currently, there are limited murine models to study C. auris infection. These models use a combination of cyclophosphamide and cortisone acetate that suppress both innate and adaptive immunity. Here we compare C. auris infection in two neutrophil depleted (ND) murine models, where innate immunity is targeted using the monoclonal antibodies 1A8 and RB6-8C5.

Copyright © 2019 American Society for Microbiology. All Rights Reserved.

Keywords: Antibiotics; Drugs Resistance; Candida auris; Animal models.

——

Simultaneous #Infection with #Enterobacteriaceae and #Pseudomonas aeruginosa harboring Multiple #Carbapenemases in a Returning #Traveler colonized with #Candida auris (AAC, abstract)

[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]

Simultaneous Infection with Enterobacteriaceae and Pseudomonas aeruginosa harboring Multiple Carbapenemases in a Returning Traveler colonized with Candida auris

Ayesha Khan, William C. Shropshire, Blake Hanson, An Q. Dinh, Audrey Wanger, Luis Ostrosky-Zeichner, Cesar A. Arias, William R. Miller

DOI: 10.1128/AAC.01466-19

 

ABSTRACT

We report our clinical experience treating a critically ill patient with polymicrobial infections due to multi-drug resistant Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in a 56 year-old woman who received healthcare in India and was also colonized by Candida auris. A precision medicine approach using whole genome sequencing revealed a multiplicity of mobile elements associated with NDM-1, NDM-5, and OXA-181 and, supplemented by susceptibility testing, guided the selection of rational antimicrobial therapy.

Copyright © 2019 American Society for Microbiology. All Rights Reserved.

Keywords: Antibiotics; Drugs Resistance; NDM1; E. Coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; Candida auris.

——

#Detection of #Candida auris #antifungal drug #resistance markers directly from #clinical skin #swabs (Antimicrob Agents Chemother., abstract)

[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]

Detection of Candida auris antifungal drug resistance markers directly from clinical skin swabs

Milena Kordalewska, Annie Lee, Yanan Zhao, David S. Perlin

DOI: 10.1128/AAC.01754-19

 

ABSTRACT

Accurate and rapid assessment of Candida auris antifungal drug resistance is crucial for effective infection prevention and control actions, and patient management. Here, performance of a molecular diagnostic platform, enabling rapid identification of FKS1 and ERG11 mutations conferring echinocandin and azole resistance, respectively, was evaluated on a panel of clinical skin swabs. Gene sequencing and antifungal susceptibility testing were used as “gold standard”. All swabs were correctly categorized as harboring wild-type or mutant C. auris.

Copyright © 2019 American Society for Microbiology. All Rights Reserved.

Keywords: Candida auris; Drugs resistance; Diagnostic tests; Echinocandins.

—–

#Isolation of #Candida auris from invasive and non-invasive samples of a #patient suffering from #vascular disease, #Italy, July 2019 (Euro Surveill., abstract)

[Source: Eurosurveillance, full page: (LINK). Abstract, edited.]

Isolation of Candida auris from invasive and non-invasive samples of a patient suffering from vascular disease, Italy, July 2019

Francesca Crea1,2, Giulia Codda2,3, Andrea Orsi4, Alberto Battaglini4, Daniele Roberto Giacobbe5, Emanuele Delfino5, Riccardo Ungaro5, Anna Marchese6

Affiliations: 1 Unità Operativa di Microbiologia, Ospedale Policlinico San Martino-IRCCS, Genoa, Italy; 2 FC and GC contributed equally to this article; 3 Microbiology Unit, DISC University of Genoa, Genoa, Italy; 4 Unità Operativa di Igiene, University of Genoa (DISSAL) and Ospedale Policlinico San Martino-IRCCS, Genoa, Italy; 5 Unità Operativa Clinica Malattie Infettive, University of Genoa (DISSAL) and Ospedale Policlinico San Martino-IRCCS, Genoa, Italy; 6 Unità Operativa di Microbiologia, University of Genoa (DISC) and Ospedale Policlinico San Martino-IRCCS, Genoa, Italy

Correspondence:  Anna Marchese

Citation style for this article: Crea Francesca, Codda Giulia, Orsi Andrea, Battaglini Alberto, Giacobbe Daniele Roberto, Delfino Emanuele, Ungaro Riccardo, Marchese Anna. Isolation of Candida auris from invasive and non-invasive samples of a patient suffering from vascular disease, Italy, July 2019. Euro Surveill. 2019;24(37):pii=1900549. https://doi.org/10.2807/1560-7917.ES.2019.24.37.1900549

Received: 02 Sep 2019;   Accepted: 11 Sep 2019

 

Abstract

We recently isolated Candida auris from a blood culture and cutaneous swabs of a patient in her mid-70s. Our routine phenotypic methods failed to identify the microorganism, but it was identified by molecular tests and MALDI-TOF MS analysis. Our report, the first from Italy, further underlines the geographically wide distribution of C. auris and the need to confirm species identification of any suspicious colony as soon as possible to stop its spread.

©  This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords: Candida auris; Nosocomial outbreaks; Italy.

——

What Is Known About #Candida auris (JAMA, summary)

[Source: Journal of the American Medical Association (JAMA), full page: (LINK). Summary, edited.]

JAMA Insights  / Clinical Update / September 6, 2019

What Is Known About Candida auris

Suzanne F. Bradley, MD1,2

Author Affiliations: 1 Infectious Diseases Division, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor; 2 Veterans Affairs Ann Arbor Healthcare System, Ann Arbor, Michigan

JAMA. Published online September 6, 2019. doi:10.1001/jama.2019.13843

___

Summary

Candida auris is a new species that was reported in Asia as a rare cause of ear infections in 2009; it had not been found among large repositories of yeast isolates collected prior to 2013.1,2 However, the widespread dissemination of C auris is not due to a single strain. For reasons that are not clear, multiple strains, called clades, have emerged independently in various parts of the world.1,2 Cases of C auris have been identified in 33 countries across 5 continents.1-3

(…)

___

Article Information

Corresponding Author: Suzanne F. Bradley, MD, Infectious Diseases Section 111i, Veterans Affairs Ann Arbor Healthcare System, 2215 Fuller Rd, Ann Arbor, MI 48105 (sbradley@umich.edu).

Published Online: September 6, 2019. doi:10.1001/jama.2019.13843

Conflict of Interest Disclosures: Dr Bradley reported receiving grants from Pfizer and the Veterans Administration and is the editor in chief for the Infection Control & Hospital Epidemiology journal.

Keywords: Candida auris; Emerging diseases; Nosocomial outbreaks; Drugs resistance; Fluconazole.

——