Detection of replicative Kashmir #Bee Virus and Black Queen Cell Virus in Asian hornet #Vespa velutina (Lepelieter 1836) in #Italy (Sci Rep., abstract)

[Source: Scientific Reports, full page: (LINK). Abstract, edited.]

Article | OPEN | Published: 12 July 2019

Detection of replicative Kashmir Bee Virus and Black Queen Cell Virus in Asian hornet Vespa velutina (Lepelieter 1836) in Italy

Maurizio Mazzei, Giovanni Cilia, Mario Forzan, Antonio Lavazza, Franco Mutinelli & Antonio Felicioli

Scientific Reports, volume 9, Article number: 10091 (2019) | Download Citation

 

Abstract

Information concerning the pathogenic role of honey bee viruses in invasive species are still scarce. The aim of this investigation was to assess the presence of several honey bee viruses, such as Black Queen Cell Virus (BQCV), Kashmir Bee Virus (KBV), Slow Paralysis Virus (SPV), Sac Brood Virus (SBV), Israeli Acute Paralysis Virus (IAPV), Acute Bee Paralysis Virus (ABPV), Chronic Bee Paralysis Virus (CBPV), in Vespa velutina specimens collected in Italy during 2017. Results of this investigation indicate that among pathogens, replicative form of KBV and BQCV were detected, assessing the spillover effect of both these viruses from managed honey bees to hornets.

Keywords: Wildlife; Honey bee viruses; Bees; Italy.

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#Microbial characterization of #bee #pollen from the #Vesuvius area collected by using three different traps (PLoS One, abstract)

[Source: PLoS One, full page: (LINK). Abstract, edited.]

OPEN ACCESS /  PEER-REVIEWED / RESEARCH ARTICLE

Microbial characterization of bee pollen from the Vesuvius area collected by using three different traps

Gianluigi Mauriello , Annachiara De Prisco, Gennaro Di Prisco, Antonietta La Storia, Emilio Caprio

Published: September 21, 2017 / DOI: https://doi.org/10.1371/journal.pone.0183208

 

Abstract

Flower pollen is collected by honeybee foragers, adhered on their rear legs and transported into the hives in the form of pellets. Once in the hives, bee pollen is moisturised with nectar and bee mouth secretions and due to enzymatically modifications it becomes the so-called bee-bread, the protein reservoir of young bees. Bee pollen can be artificially removed from bee legs and collected by using specific systems, the bee pollen traps. Bee pollen is commercialized for human consumption as fresh product and after freezing or drying. Although bee pollen is nowadays largely consumed in developed countries, as food or food supplement according to local legislation, little is known on its safety related to microbiological hazards. In this work, we aimed to characterize for the first time the microbiological profile of Italian bee pollen in fresh, frozen and dried form collected along an entire harvesting season. Moreover, monthly microbiological analyses were performed on frozen (storage at -18°C) and dried (storage at room temperature) bee pollen over a 4 months period. Further aim of this work was the evaluation of the possible impact on production level of three different traps used for pollen collection. Our results on microbial contamination of fresh and frozen bee pollen show that a more comprehensive microbiological risk assessment of bee pollen is required. On the other side, dried pollen showed very low microbial contamination and no pathogen survived after the drying process and during storage.

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Citation: Mauriello G, De Prisco A, Di Prisco G, La Storia A, Caprio E (2017) Microbial characterization of bee pollen from the Vesuvius area collected by using three different traps. PLoS ONE 12(9): e0183208. https://doi.org/10.1371/journal.pone.0183208

Editor: Massimiliano Galdiero, Seconda Universita degli Studi di Napoli, ITALY

Received: August 31, 2016; Accepted: July 20, 2017; Published: September 21, 2017

Copyright: © 2017 Mauriello et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: Sequencing data were deposited in the GeneBank database of the NCBI (accession numbers are reported in Table 5).

Funding: The authors received no specific funding for this work.

Competing interests: The authors have declared that no competing interests exist.

Keywords: Bees; Bacterial Contamination; Bee Pollen.

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