[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Virol J. 2020 Apr 29;17(1):62. doi: 10.1186/s12985-020-01331-z.
Co-circulation of Multiple Reassortant H6 Subtype Avian Influenza Viruses in Wild Birds in Eastern China, 2016-2017
Chuanxia Hu 1, Xiaofang Li 1, Caihui Zhu 1, Feng Zhou 2, Wangjun Tang 1, Di Wu 3, Zhihui Li 1, Lichen Zhou 4, Jing Liu 1, Xiaoman Wei 5 6 7, Jie Cui 5 6, Tianhou Wang 8 9, Guimei He 10 11
Affiliations: 1 School of Life Sciences, East China Normal University, Shanghai, China. 2 Jinshan Forest Working-Station, Shanghai, China. 3 Shanghai Wildlife Conservation and Management Center, Shanghai, China. 4 Shanghai Zoo, Shanghai, China. 5 Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. 6 Unit of Pathogen Bioinformatics, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China. 7 University of Chinese Academy of Sciences, Beijing, China. 8 School of Life Sciences, East China Normal University, Shanghai, China. Thwang@bio.ecnu.edu.cn. 9 Institute of Eco-Chongming (IEC), East China Normal University, Shanghai, China. Thwang@bio.ecnu.edu.cn. 10 School of Life Sciences, East China Normal University, Shanghai, China. firstname.lastname@example.org. 11 Institute of Eco-Chongming (IEC), East China Normal University, Shanghai, China. email@example.com.
PMID: 32349760 DOI: 10.1186/s12985-020-01331-z
H6 subtype influenza viruses were prevalent in domestic poultry and wild birds, which also could pose potential threat to humans. However, little is known about the prevalence of H6 subtype viruses in wild birds in eastern China, a crucial stopover or wintering site for migratory wild birds along the East Asian-Australasian Flyway.
During the routine surveillance in 2016-2017, H6 subtype AIVs positive samples were identified, and the representative strains were selected for further sequence and phylogenetic analysis and the pathogenicity in mice were evaluated.
Among the 30 H6 positive samples, there were at least four subtypes H6N1, H6N2, H6N5 and H6N8 co-circulated in Shanghai, China. Genetic analysis showed the 8 representative isolates shared homology with different AIV sub-lineages isolated from domestic ducks or wild birds in different countries along the East Asian-Australasian flyways, and were classified into 7 new genotypes. The pathogenicity to mice showed that these H6 viruses could replicate efficiently in the lungs without prior adaptation, but could not cause mice death.
Eight novel strains belonged to H6N1, H6N2, H6N5 and H6N8 subtypes were isolated. Phylogenetic analyses revealed multiple origins of internal genes indicative of robust reassortment events and frequent wild birds-poultry interaction encouraging the evolution and emergence of new genotypes. The pathogenicity to mammals should be closely monitored to prevent the emergence of novel pandemic viruses.
Keywords: Eastern China; H6 subtype avian influenza virus; Novel; Reassortant; Shanghai; Wild birds.
Keywords: Avian Influenza; H6N1; H6N2; H6N5; H6N8; Reassortant strain; Wild Birds; China.