[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Heliyon. 2020 Jun 6;6(6):e04068. doi: 10.1016/j.heliyon.2020.e04068. eCollection 2020 Jun.
Molecular Characterization and Three-Dimensional Structures of Avian H8, H11, H14, H15 and Swine H4 Influenza Virus Hemagglutinins
Hua Yang 1, Paul J Carney 1, Jessie C Chang 1, James Stevens 1
Affiliation: 1 Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
PMID: 32529072 PMCID: PMC7281811 DOI: 10.1016/j.heliyon.2020.e04068
Of the eighteen hemagglutinin (HA) subtypes (H1-H18) that have been identified in bats and aquatic birds, many HA subtypes have been structurally characterized. However, several subtypes (H8, H11 and H12) still require characterization. To better understand all of these HA subtypes at the molecular level, HA structures from an A(H4N6) (A/swine/Missouri/A01727926/2015), an A(H8N4) (A/turkey/Ontario/6118/1968), an A(H11N9) (A/duck/Memphis/546/1974), an A(H14N5) A/mallard/Gurjev/263/1982, and an A(H15N9) (A/wedge-tailed shearwater/Western Australia/2576/1979 were determined by X-ray crystallography at 2.2Å, 2.3Å, 2.8Å, 3.0Å and 2.5Å resolution, respectively. The interactions between these viruses and host receptors were studied utilizing glycan-binding analyses with their recombinant HA. The data show that all avian HAs retain their strict binding preference to avian receptors, whereas swine H4 has a weak human receptor binding. The molecular characterization and structural analyses of the HA from these zoonotic influenza viruses not only provide a deeper appreciation and understanding of the structure of all HA subtypes, but also re-iterate why continuous global surveillance is needed.
Keywords: A(H11N9); A(H14N5); A(H15N9); A(H4N6); A(H8N4); Avian; Biomolecules; Glycobiology; Hemagglutinin; Influenza virus; Microbiology; Proteins; Receptor binding; Swine; Viral protein; Virology.
Keywords: Influenza A; Avian Influenza; Swine Influenza; H8N4; H4N6; H11N9; H14N5; H15N9.