[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
BMC Neurosci. 2018 Feb 20;19(1):5. doi: 10.1186/s12868-018-0407-2.
Characterisation of Zika virus infection in primary human astrocytes.
Stefanik M1, Formanova P1, Bily T2,3, Vancova M2,3, Eyer L1,2, Palus M1,2, Salat J1, Braconi CT4, Zanotto PMA4, Gould EA5, Ruzek D6,7.
Author information: 1 Department of Virology, Veterinary Research Institute, Hudcova 70, 62100, Brno, Czech Republic. 2 Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 31, 37005, Ceske Budejovice, Czech Republic. 3 Faculty of Science, University of South Bohemia, Branisovska 31, 37005, Ceske Budejovice, Czech Republic. 4 Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Institute of Microbiology Sciences, University of São Paulo, São Paulo, 05508-000, Brazil. 5 EHESP French School of Public Health, IRD French Institute of Research for Development, EPV UMR_D 190 Emergence des Pathologies Virales, Aix Marseille Université, Marseille, France. 6 Department of Virology, Veterinary Research Institute, Hudcova 70, 62100, Brno, Czech Republic. firstname.lastname@example.org. 7 Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 31, 37005, Ceske Budejovice, Czech Republic. email@example.com.
The recent Zika virus (ZIKV) outbreak has linked ZIKV with microcephaly and other central nervous system pathologies in humans. Astrocytes are among the first cells to respond to ZIKV infection in the brain and are also targets for virus infection. In this study, we investigated the interaction between ZIKV and primary human brain cortical astrocytes (HBCA).
HBCAs were highly sensitive to representatives of both Asian and African ZIKV lineages and produced high viral yields. The infection was associated with limited immune cytokine/chemokine response activation; the highest increase of expression, following infection, was seen in CXCL-10 (IP-10), interleukin-6, 8, 12, and CCL5 (RANTES). Ultrastructural changes in the ZIKV-infected HBCA were characterized by electron tomography (ET). ET reconstructions elucidated high-resolution 3D images of the proliferating and extensively rearranged endoplasmic reticulum (ER) containing viral particles and virus-induced vesicles, tightly juxtaposed to collapsed ER cisternae.
The results confirm that human astrocytes are sensitive to ZIKV infection and could be a source of proinflammatory cytokines in the ZIKV-infected brain tissue.
KEYWORDS: Astrocyte; Electron tomography; Flavivirus; Immune response; Luminex; Zika virus
PMID: 29463209 DOI: 10.1186/s12868-018-0407-2
Keywords: Zika Virus; Neuroinvasion.