[Source: Memorias do Instituto Oswaldo Cruz, full page: (LINK). Abstract, edited.]
Genomic and phylogenetic characterization of an imported case of SARS-CoV-2 in Amazonas State, Brazil
[ACCEPTED ARTICLES / PRELIMINARY VERSION]
Valdinete Alves do Nascimento1,2, André Lima Guerra Corado1,2, Fernanda Oliveira do Nascimento1,3, Ágatha Kelly Araújo da Costa1,3, Debora Camila Gomes Duarte1, Michele Silva de Jesus 1, Sérgio Luiz Bessa Luz1,3, Luciana Mara Fé Gonçalves4, Cristiano Fernandes da Costa4, Edson Delatorre5, Felipe Gomes Naveca1,2,3,6,+
1 Instituto Leônidas e Maria Deane, Fiocruz, Amazonas, Brasil; 2 Programa de Pós-Graduação em Biologia Celular e Molecular Fundação, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brasil; 3 Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro, Instituto Leônidas e Maria Deane, Amazonas, Brasil; 4 Fundação de Vigilância em Saúde do Amazonas, Amazonas, Brasil; 5 Departamento de Biologia, Centro de Ciências Exatas, Naturais e da Saúde, Universidade Federal do Espírito Santo, Espírito Santo, Brasil; 6 Rede Genômica em Saúde do Estado do Amazonas, Amazonas, Brasil
DOI: 10.1590/0074-02760200310
ABSTRACT
A new coronavirus (SARS-CoV-2) is currently causing a life-threatening pandemic. In this study, we report the complete genome sequencing and genetic characterization of a SARS-CoV-2 detected in Manaus, Amazonas, Brazil, and the protocol we designed to generate high-quality SARS-CoV-2 full genome data. The isolate was obtained from an asymptomatic carrier returning from Madrid, Spain. Nucleotide sequence analysis showed a total of nine mutations in comparison with the original Human case in Wuhan, China, and support this case as belonging to the recently proposed lineage A.2. Phylogeographic analysis further confirmed the likely European origin of this case. To our knowledge, this is the first SARS-CoV-2 genome obtained from the North Brazilian Region. We believe that the information generated in this study may contribute to the ongoing efforts toward the SARS-CoV-2 emergence.
key words: SARS-CoV-2 genome COVID-19 Coronavirus Brazil Amazon region
+ Corresponding author: felipe.naveca@fiocruz.br
Received 14 June 2020 – Accepted 15 June 2020
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Keywords: SARS-CoV-2; COVID-19; Brazil; Genetics.
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