Studying #Evolutionary #Adaptation of #MERS-CoV (Methods Mol Biol., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Methods Mol Biol. 2020;2099:3-8. doi: 10.1007/978-1-0716-0211-9_1.

Studying Evolutionary Adaptation of MERS-CoV.

Letko M1, Munster V2.

Author information: 1 Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institutes of Health, Hamilton, MT, USA. michael.letko@nih.gov. 2 Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institutes of Health, Hamilton, MT, USA.

 

Abstract

Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730-1737, 2018.

KEYWORDS: Cell culture; Experimental evolution; Forced adaptation; Host restriction; MERS-CoV; Semi-permissive cell line; Species barrier

PMID: 31883083 DOI: 10.1007/978-1-0716-0211-9_1

Keywords: MERS-CoV; Evolution.

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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.