#Cytokine Induction in #Nipah Virus–Infected Primary #Human and #Porcine #Bronchial Epithelial Cells (J Infect Dis., abstract)

[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]

Cytokine Induction in Nipah Virus–Infected Primary Human and Porcine Bronchial Epithelial Cells

Mareike Elvert, Lucie Sauerhering, Andrea Maisner

The Journal of Infectious Diseases, jiz455, https://doi.org/10.1093/infdis/jiz455

Published: 30 October 2019

 

Abstract

During the Nipah virus (NiV) outbreak in Malaysia, pigs and humans were infected. While pigs generally developed severe respiratory disease due to an effective virus replication and associated inflammation processes in the porcine airways, respiratory symptoms in humans were rare and less severe. To elucidate the reasons for the species-specific differences in NiV airway infections, we compared the cytokine responses as a first reaction to NiV in primary porcine and human bronchial epithelial cells (PBEpC and HBEpC, respectively). In both cell types, NiV infections resulted in the expression of type III interferons (IFN-λ). Upon infections with similar virus doses, the viral RNA load and IFN expression were substantially higher in HBEpC. Even if PBEpC expressed the same viral RNA amounts as NiV-infected HBEpC, the porcine cells showed a reduced IFN- and IFN-dependent antiviral gene expression. Despite this inherently limited IFN response, the expression of proinflammatory cytokines (IL-6, IL-8) in NiV-infected PBEpC was not decreased. The downregulation of the antiviral activity in the presence of a functional proinflammatory cytokine response might be one of the species-specific factors contributing to efficient virus replication and acute inflammation in the lungs of pigs infected with the Malaysian NiV strain.

Nipah virus, primary bronchial epithelia, IFN-λ

Topic: cytokine – inflammation – lung – antiviral agents – disease outbreaks –  interferons – interleukin-8 – malaysia – rna, viral – signs and symptoms, respiratory – suidae – virus replication – infection – interleukin-6 – viruses – epithelial cells – airway device – nipah virus – nipah virus infections

Issue Section: Supplement Article

© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Keywords: Nipah virus; Cytokines; Immunopathology; Viral pathogenesis.

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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.