[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]
Cytokine Induction in Nipah Virus–Infected Primary Human and Porcine Bronchial Epithelial Cells
Mareike Elvert, Lucie Sauerhering, Andrea Maisner
The Journal of Infectious Diseases, jiz455, https://doi.org/10.1093/infdis/jiz455
Published: 30 October 2019
During the Nipah virus (NiV) outbreak in Malaysia, pigs and humans were infected. While pigs generally developed severe respiratory disease due to an effective virus replication and associated inflammation processes in the porcine airways, respiratory symptoms in humans were rare and less severe. To elucidate the reasons for the species-specific differences in NiV airway infections, we compared the cytokine responses as a first reaction to NiV in primary porcine and human bronchial epithelial cells (PBEpC and HBEpC, respectively). In both cell types, NiV infections resulted in the expression of type III interferons (IFN-λ). Upon infections with similar virus doses, the viral RNA load and IFN expression were substantially higher in HBEpC. Even if PBEpC expressed the same viral RNA amounts as NiV-infected HBEpC, the porcine cells showed a reduced IFN- and IFN-dependent antiviral gene expression. Despite this inherently limited IFN response, the expression of proinflammatory cytokines (IL-6, IL-8) in NiV-infected PBEpC was not decreased. The downregulation of the antiviral activity in the presence of a functional proinflammatory cytokine response might be one of the species-specific factors contributing to efficient virus replication and acute inflammation in the lungs of pigs infected with the Malaysian NiV strain.
Nipah virus, primary bronchial epithelia, IFN-λ
Topic: cytokine – inflammation – lung – antiviral agents – disease outbreaks – interferons – interleukin-8 – malaysia – rna, viral – signs and symptoms, respiratory – suidae – virus replication – infection – interleukin-6 – viruses – epithelial cells – airway device – nipah virus – nipah virus infections
Issue Section: Supplement Article
© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: firstname.lastname@example.org.
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Keywords: Nipah virus; Cytokines; Immunopathology; Viral pathogenesis.