[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Infect Drug Resist. 2019 Oct 3;12:3113-3124. doi: 10.2147/IDR.S206691. eCollection 2019.
Characterization of phenotypic and genotypic traits of carbapenem-resistant Acinetobacter baumannii clinical isolates recovered from a tertiary care hospital in Taif, Saudi Arabia.
El-Badawy MF1,2, Abdelwahab SF1,3, Alghamdi SA4, Shohayeb MM1,5.
Author information: 1 Division of Pharmaceutical Microbiology, Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University, Taif 21974, Kingdom of Saudi Arabia. 2 Department of Microbiology and Immunology, Faculty of Pharmacy, Misr University for Science and Technology, 6th of October City 12568, Egypt. 3 Department of Microbiology and Immunology, Faculty of Medicine, Minia University, Minia 61511, Egypt. 4 Medical Genetics, Clinical Laboratory Department, College of Applied Medical Sciences, Taif University, Taif 21974, Kingdom of Saudi Arabia. 5 Department of Microbiology and Biotechnology, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa 35712, Egypt.
BACKGROUND AND OBJECTIVE:
Acinetobacter baumannii (A. baumannii) is a common nosocomial pathogen, which developed multi-drug-resistance to different classes of antibiotics including carbapenems. This study examined ten common carbapenemase genes among 32 carbapenem-resistant A. baumannii clinical isolates recovered from Taif, Saudi Arabia.
Isolates were phenotypically identified to the genus level by Vitek®2 and API 20NE®. The species level was confirmed by the amplification of bla OXA-51. The susceptibility for 21 different antibiotics was performed by Vitek 2 and modified Kirby-Bauer method. Isolates were genetically screened for 10 carbapenemases. Phylogenetic relatedness between isolates was determined by ERIC-PCR.
Genotypically identified A. baumannii represented 100% of the total phenotypically identified Acinetobacter spp. All the carbapenem-resistant isolates were sensitive to polymyxin B and colistin. Among the other antibiotics, ampicillin/sulbactam and tigecycline were the most effective agents. 90.8% of the isolates were resistant to all ten investigated β-lactams. bla OXA-51, bla IPM, bla NDM and bla OXA-23 were detected in 100%, 87.5%, 62.5% and 59.4% of isolates, respectively. Also, bla VIM and bla OXA-40 were less prevalent and were detected in 9.3% and 3.1% of the isolates, respectively. In addition, bla KPC, bla OXA-48, bla OXA-58, bla OXA-181 were not detected in any isolate. The A. baumannii isolates were categorised into ten genotypes on the basis of the detected carbapenemase genes and ERIC-PCR revealed a remarkable clonal diversity among these isolates.
Class A and class D carbapenemase genes were the most commonly detected among carbapenem resistant A. baumannii (CRAB) clinical isolates.
© 2019 El-Badawy et al.
KEYWORDS: A. baumannii; ERIC-PCR; blaOXA-51; carbapenemases; carbapenems
PMID: 31632100 PMCID: PMC6781848 DOI: 10.2147/IDR.S206691
Keywords: Antibiotics; Drugs Resistance; Carbapenem; Acinetobacter baumannii; Nosocomial outbreaks; Saudi Arabia.