Comparison of #WGS of #Legionella pneumophila in #Tap #Water and in #Clinical Strains, #Flint, #Michigan, #USA, 2016 (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 25, Number 11—November 2019 / Research

Comparison of Whole-Genome Sequences of Legionella pneumophila in Tap Water and in Clinical Strains, Flint, Michigan, USA, 2016

Emily Garner, Connor L. Brown, David Otto Schwake, William J. Rhoads, Gustavo Arango-Argoty, Liqing Zhang, Guillaume Jospin, David A. Coil, Jonathan A. Eisen, Marc A. Edwards, and Amy Pruden

Author affiliations: West Virginia University, Morgantown, West Virginia, USA (E. Garner); Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA (E. Garner, C.L. Brown, W.J. Rhoads, G. Arango-Argoty, L. Zhang, M.A. Edwards, A. Pruden); Methodist University, Fayetteville, North Carolina, USA (D.O. Schwake); University of California at Davis, Davis, California, USA (G. Jospin, D.A. Coil, J.A. Eisen)

 

Abstract

During the water crisis in Flint, Michigan, USA (2014–2015), 2 outbreaks of Legionnaires’ disease occurred in Genesee County, Michigan. We compared whole-genome sequences of 10 clinical Legionella pneumophila isolates submitted to a laboratory in Genesee County during the second outbreak with 103 water isolates collected the following year. We documented a genetically diverse range of L. pneumophila strains across clinical and water isolates. Isolates belonging to 1 clade (3 clinical isolates, 3 water isolates from a Flint hospital, 1 water isolate from a Flint residence, and the reference Paris strain) had a high degree of similarity (2–1,062 single-nucleotide polymorphisms), all L. pneumophila sequence type 1, serogroup 1. Serogroup 6 isolates belonging to sequence type 2518 were widespread in Flint hospital water samples but bore no resemblance to available clinical isolates. L. pneumophila strains in Flint tap water after the outbreaks were diverse and similar to some disease-causing strains.

Keywords: Legionellosis; USA; Michigan.

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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.