[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
BMC Res Notes. 2019 Oct 1;12(1):639. doi: 10.1186/s13104-019-4671-8.
Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus.
Oliver GF1, Orang AV1, Appukuttan B1, Marri S1, Michael MZ1, Marsh GA2, Smith JR3.
Author information: 1 Flinders University College of Medicine and Public Health, Flinders Medical Centre Room 4E-431, Flinders Drive, Bedford Park, SA, 5042, Australia. 2 Health and Biosecurity, Commonwealth Scientific and Industrial Research Organisation, 5 Portarlington Rd, Newcomb, VIC, 3219, Australia. 3 Flinders University College of Medicine and Public Health, Flinders Medical Centre Room 4E-431, Flinders Drive, Bedford Park, SA, 5042, Australia. email@example.com.
Survivors of Ebola virus disease (EVD) are at risk of developing blinding intraocular inflammation-or uveitis-which is associated with retinal pigment epithelial (RPE) scarring and persistence of live Zaire ebolavirus (EBOV) within the eye. As part of a large research project aimed at defining the human RPE cell response to being infected with EBOV, this work focused on the microRNAs (miRNAs) associated with the infection.
Using RNA-sequencing, we detected 13 highly induced and 2 highly repressed human miRNAs in human ARPE-19 RPE cells infected with EBOV, including hsa-miR-1307-5p, hsa-miR-29b-3p and hsa-miR-33a-5p (up-regulated), and hsa-miR-3074-3p and hsa-miR-27b-5p (down-regulated). EBOV-miR-1-5p was also found in infected RPE cells. Through computational identification of putative miRNA targets, we predicted a broad range of regulatory activities, including effects on innate and adaptive immune responses, cellular metabolism, cell cycle progression, apoptosis and autophagy. The most highly-connected molecule in the miR-target network was leucine-rich repeat kinase 2, which is involved in neuroinflammation and lysosomal processing. Our findings should stimulate new studies on the impact of miRNA changes in EBOV-infected RPE cells to further understanding of intraocular viral persistence and the pathogenesis of uveitis in EVD survivors.
KEYWORDS: Ebola; Filovirus; Retina; Retinal pigment epithelium; Uveitis; Zaire ebolavirus; microRNA
PMID: 31570108 DOI: 10.1186/s13104-019-4671-8
Keywords: Ebola; ZEBOV; Viral pathogenesis.