Efficacy of Active #Immunization with Attenuated Alpha-Hemolysin and PVL in a Rabbit Model of #Staphylococcus aureus Necrotizing #Pneumonia (J Infect Dis., abstract)

[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]

Efficacy of Active Immunization with Attenuated Alpha-Hemolysin and Panton-Valentine Leukocidin in a Rabbit Model of Staphylococcus aureus Necrotizing Pneumonia

Vuvi G Tran, Arundhathi Venkatasubramaniam, Rajan P Adhikari,Subramaniam Krishnan, Xing Wang, Vien T M Le, Hoan N Le, Trang T T Vu,Erika Schneider-Smith, M Javad Aman, Binh An Diep

The Journal of Infectious Diseases, jiz437, https://doi.org/10.1093/infdis/jiz437

Published: 26 August 2019

 

Abstract

Staphylococcus aureus is a common pathogen causing infections in humans with various degrees of severity, with pneumonia being one of the most severe infections. In as much as staphylococcal pneumonia is a disease driven in large part by alpha-hemolysin (Hla) and Panton-Valentine leukocidin (PVL), we evaluated whether active immunization with attenuated forms of Hla (HlaH35L/H48L) alone, PVL components (LukS-PVT28F/K97A/S209A and LukF-PVK102A) alone, or combination of all three toxoids could prevent lethal challenge in a rabbit model of necrotizing pneumonia caused by the USA300 community-associated methicillin-resistant S. aureus. Rabbits vaccinated with Hla toxoid alone or PVL components alone were only partially protected against lethal pneumonia, whereas those vaccinated with all three toxoids had 100% protection against lethality. Vaccine-mediated protection correlated with induction of polyclonal antibody response that neutralized not only alpha-hemolysin and PVL, but also other related toxins, produced by USA300 and other epidemic MRSA clones.

MRSA, pneumonia, alpha-toxin, PVL, antibodies, vaccine

Topic: staphylococcus aureus – immunization, active – human leukocyte antigens – clone cells – hemolysin – methicillin – pneumonia – staphylococcal pneumonia – oryctolagus cuniculus – toxins – toxoids – vaccination – vaccines – infection – antibodies – polyclonal antibody – pathogenic organism – pneumonia, necrotizing – epidemics – methicillin-resistant staphylococcus aureus – pathogenicity – alpha-toxin – panton-valentine leukocidin – community – methicillin-resistant staphylococcal aureus pneumonia – attenuation

Issue Section: Major Article

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© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

Keywords: Staphylococcus aureus; MRSA; Pneumonia; Vaccines; Animal models.

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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.