[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]
Efficacy of Active Immunization with Attenuated Alpha-Hemolysin and Panton-Valentine Leukocidin in a Rabbit Model of Staphylococcus aureus Necrotizing Pneumonia
Vuvi G Tran, Arundhathi Venkatasubramaniam, Rajan P Adhikari,Subramaniam Krishnan, Xing Wang, Vien T M Le, Hoan N Le, Trang T T Vu,Erika Schneider-Smith, M Javad Aman, Binh An Diep
The Journal of Infectious Diseases, jiz437, https://doi.org/10.1093/infdis/jiz437
Published: 26 August 2019
Staphylococcus aureus is a common pathogen causing infections in humans with various degrees of severity, with pneumonia being one of the most severe infections. In as much as staphylococcal pneumonia is a disease driven in large part by alpha-hemolysin (Hla) and Panton-Valentine leukocidin (PVL), we evaluated whether active immunization with attenuated forms of Hla (HlaH35L/H48L) alone, PVL components (LukS-PVT28F/K97A/S209A and LukF-PVK102A) alone, or combination of all three toxoids could prevent lethal challenge in a rabbit model of necrotizing pneumonia caused by the USA300 community-associated methicillin-resistant S. aureus. Rabbits vaccinated with Hla toxoid alone or PVL components alone were only partially protected against lethal pneumonia, whereas those vaccinated with all three toxoids had 100% protection against lethality. Vaccine-mediated protection correlated with induction of polyclonal antibody response that neutralized not only alpha-hemolysin and PVL, but also other related toxins, produced by USA300 and other epidemic MRSA clones.
MRSA, pneumonia, alpha-toxin, PVL, antibodies, vaccine
Topic: staphylococcus aureus – immunization, active – human leukocyte antigens – clone cells – hemolysin – methicillin – pneumonia – staphylococcal pneumonia – oryctolagus cuniculus – toxins – toxoids – vaccination – vaccines – infection – antibodies – polyclonal antibody – pathogenic organism – pneumonia, necrotizing – epidemics – methicillin-resistant staphylococcus aureus – pathogenicity – alpha-toxin – panton-valentine leukocidin – community – methicillin-resistant staphylococcal aureus pneumonia – attenuation
Issue Section: Major Article
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Keywords: Staphylococcus aureus; MRSA; Pneumonia; Vaccines; Animal models.