[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Rev Inst Med Trop Sao Paulo. 2019 Aug 19;61:e40. doi: 10.1590/S1678-9946201961040.
Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil.
Vieira DS1,2,3, Zambenedetti MR4, Requião L4, Borghetti IA4,5, Luna LKS4, Santos AOD1,3, Taborda RLM3, Pereira DB3, Krieger MA4,6, Salcedo JMV1,3, Rampazzo RCP4.
Author information: 1 Fundação Oswaldo Cruz Rondônia, Porto Velho, Rondônia, Brazil. 2 Universidade Federal de Rondônia, Programa de Pós-Graduação em Biologia Experimental, Porto Velho, Rondônia, Brazil. 3 Centro de Pesquisa em Medicina Tropical, Porto Velho, Rondônia, Brazil. 4 Instituto de Biologia Molecular do Paraná, Curitiba, Paraná, Brazil. 5 Universidade Federal do Paraná, Departamento de Engenharia de Bioprocessos e Biotecnologia, Curitiba, Paraná, Brazil. 6 Instituto Carlos Chagas, Curitiba, Paraná, Brazil.
Several arboviruses have emerged and/or re-emerged in North, Central and South-American countries. Viruses from some regions of Africa and Asia, such as the Zika and Chikungunya virus have been introduced in new continents causing major public health problems. The aim of this study was to investigate the presence of RNA from Zika, Dengue and Chikungunya viruses in symptomatic patients from Rondonia, where the epidemiological profile is still little known, by one-step real-time RT-PCR. The main clinical signs and symtoms were fever (51.2%), headache (78%), chills (6.1%), pruritus (12.2%), exanthema (20.1%), arthralgia (35.3%), myalgia (26.8%) and retro-orbital pain (19.5%). Serum from 164 symptomatic patients were collected and tested for RNA of Zika, Dengue types 1 to 4 and Chikungunya viruses, in addition to antibodies against Dengue NS1 antigen. Direct microscopy for Malaria was also performed. Only ZIKV RNA was detected in 4.3% of the patients, and in the remaining 95.7% of the patients RNA for Zika, Dengue and Chikungunya viruses were not detected. This finding is intriguing as the region has been endemic for Dengue for a long time and more recently for Chikungunya virus as well. The results indicated that medical and molecular parameters obtained were suitable to describe the first report of symptomatic Zika infections in this region. Furthermore, the low rate of detection, compared to clinical signs and symptoms as the solely diagnosis criteria, suggests that molecular assays for detection of viruses or other pathogens that cause similar symptoms should be used and the corresponding diseases could be included in the compulsory notification list.
PMID: 31432989 DOI: 10.1590/S1678-9946201961040
Keywords: Zika Virus; Dengue fever; Chikungunya fever; Seroprevalence; Brazil.