#Evolution of #pandemic #influenza virus A #H1N1pdm09 in 2009-2016: dynamics of #receptor specificity of the first #hemagglutinin subunit (HA1) (Vopr Virusol., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Vopr Virusol. 2019;64(2):63-72. doi: 10.18821/0507-4088-2019-64-2-63-72.

[Evolution of pandemic influenza virus A(H1N1)pdm09 in 2009-2016: dynamics of receptor specificity of the first hemagglutinin subunit (HA1).]

[Article in Russian]

Lvov DK1, Bogdanova VS1, Kirillov IM1, Shchelkanov MY2, Burtseva EI1, Bovin NV3, Fedyakina IT1, Prilipov AG1, Alhovsky SV1, Samokhvalov EI1, Proshina ES1, Kirillova ES1, Syroeshkin AV4.

Author information: 1 Ivanovsky Institute of Virology «National Research Centre for Epidemiology and Microbiology named honorary academician N.F. Gamaleya», Moscow, 123098, Russian Federation. 2 Far Eastern Federal University, Vladivostok, 690950, Primorsky Krai, Russian Federation. 3 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russian Federation. 4 Peoples Frendship University of Russia (RUDN), Moscow, 117198, Russian Federation.




The new reassortant of the swine flu virus A(H1N1)pdm09, which emerged in 2009, overcame the species barrier and caused the 2009-2010 pandemic. One of the key points required for the influenza virus to overcome the species barrier and adapt it to humans is its specific binding to the receptors on the epithelium of the human respiratory tract.


Studying the dynamics of changes in receptor specificity (RS) of the HA1 subunit of the hemagglutinin of the influenza A(H1N1)pdm09 virus strains isolated during the period 2009-2016 on the territory of the Russian Federation, and an analysis of the possible impact of these changes on the incidence rates of the population of the Russian Federation of pandemic influenza in certain epidemic seasons.


Standard methods of collecting clinical materials, isolation of influenza viruses, their typing and genome sequencing were used. For the study of RS of influenza A virus (H1N1)pdm09, the method of solid phase sialosidenzyme analysis was used.


It is shown that the change in the parameter W3/6 , which characterizes the degree of a2-3 receptor specificity (a2-3-RS) of the influenza virus A(H1N1) pdm09 over a2-6-RS, coincides with the change in the incidence rates of the Russian Federation’s pandemic flu in separate epidemic seasons. There is a tendency to increase the affinity of the virus A(H1N1)pdm09 to α2-3 analogs of the sialyl-glycan receptors of the human respiratory tract epithelium – α2-3-sialoglycopolymers (α2-3-SGP), and falls to α2-6-SGP, with the virus showing the greatest affinity for sulfated sialoglycopolymers.


Screening for RS strains of influenza A (H1N1)pdm09 virus isolated on the territory of the Russian Federation in 2009-2016 revealed a decrease in the affinity of viruses for a2-6-sialosides, especially for 6’SL-SGP, which is probably due to the presence of amino acid substitutions in the 222 and 223 positions of RBS HA1 viruses. Previous studies have shown that the presence of such substitutions correlates with an increase in the virulence of the influenza A virus (H1N1)pdm09 [16, 23]. Probably, the pandemic virus has evolved towards the selection of more virulent pneumotropic variants.


Monitoring of the receptor specificity of a pandemic influenza virus makes it possible to identify strains with altered RS to the epithelium of the human respiratory tract and an increased ability to transfer from person to person. Change in the period 2009-2016 the W3/6parameter characterizing the degree of α2-3-RS excess of the influenza A(H1N1)pdm09 virus over α2-6-RS, coincides with the change in the incidence rates of the pandemic influenza population of the Russian Federation in certain epidemic seasons.

KEYWORDS: epidemic season; hemagglutinin (HA); influenza; influenza virus A(H1N1)pdm09; receptor specificity; sialoglycopolymer (SGP); sialyl-glycan receptor

PMID: 31412172 DOI: 10.18821/0507-4088-2019-64-2-63-72

Keywords: Seasonal Influenza; H1N1pdm09; Russia.


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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.