[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Virology. 2019 Jul 14;535:227-231. doi: 10.1016/j.virol.2019.07.013. [Epub ahead of print]
Infectious cDNA clones of two strains of Mayaro virus for studies on viral pathogenesis and vaccine development.
Chuong C1, Bates TA1, Weger-Lucarelli J2.
Author information: 1 Department of Biomedical Sciences and Pathobiology, Virginia Tech, VA-MD Regional College of Veterinary Medicine, Blacksburg, VA, USA. 2 Department of Biomedical Sciences and Pathobiology, Virginia Tech, VA-MD Regional College of Veterinary Medicine, Blacksburg, VA, USA. Electronic address: email@example.com.
Mayaro virus (MAYV; family Togaviridae, genus Alphavirus) is an emerging global threat that can cause severe clinical manifestations similar to Zika, dengue, and chikungunya viruses. Currently, there is a lack of molecular tools to enable a better understanding of the transmission and pathogenesis of MAYV. Here, we detail the development and characterization of infectious clones of two strains of MAYV that produce infectious virus and replicate in mammalian and mosquito cells similarly to wild-type virus. Additionally, clone-derived viruses produced identical infection rates and phenotypes in CD-1 mice compared to the parental strains. This infectious clone system will provide a resource to the research community to analyze MAYV genetic determinants of virulence, determine vector competence, and develop vaccines.
Copyright © 2019 Elsevier Inc. All rights reserved.
KEYWORDS: Alphavirus; Infectious clones; Mayaro virus
PMID: 31325837 DOI: 10.1016/j.virol.2019.07.013
Keywords: Alphavirus; Togavirus; Mayaro virus; Viral pathogenesis.