Natural #Vertical #Transmission of #Zika Virus in #Larval #Aedes aegypti Populations, Morelos, #Mexico (Emerg Infect Dis., abstract)

[Source: US Centers for Disease Control and Prevention (CDC), Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]

Volume 25, Number 8—August 2019 / Research

Natural Vertical Transmission of Zika Virus in Larval Aedes aegypti Populations, Morelos, Mexico

Mónica Izquierdo-Suzán, Selene Zárate, Jesús Torres-Flores, Fabián Correa-Morales, Cassandra González-Acosta, Edgar E. Sevilla-Reyes, Rosalia Lira, Sofía L. Alcaraz-Estrada, and Martha Yocupicio-Monroy

Author affiliations: Universidad Autónoma de la Ciudad de Mexico, Mexico City, Mexico (M. Izquierdo-Suzán, S. Zárate, M. Yocupicio-Monroy); Instituto Politécnico Nacional, Mexico City (J. Torres-Flores); Centro Nacional de Programas Preventivos y Control de Enfermedades, Mexico City (F. Correa-Morales, C. González-Acosta); Instituto Nacional de Enfermedades Respiratorias. Mexico City (E.E. Sevilla-Reyes); Instituto Mexicano del Seguro Social, Mexico City (R. Lira); Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, Mexico City (S.L. Alcaraz-Estrada)

 

Abstract

We characterized natural vertical transmission of Zika virus in pools of Aedes aegypti larvae hatched from eggs collected in Jojutla, Morelos, Mexico. Of the 151 pools analyzed, 17 tested positive for Zika virus RNA; infectious Zika virus was successfully isolated from 1 of the larvae pools (31N) in C6/36 cells. Real-time quantitative PCR and indirect immunofluorescence assays confirmed the identity of the isolate, named Zika virus isolate 31N; plaque assays in Vero cells demonstrated the isolate’s infectivity in a mammalian cell line. We obtained the complete genome of Zika virus isolate 31N by next-generation sequencing and identified 3 single-nucleotide variants specific to Zika virus isolate 31N using the meta-CATS tool. These results demonstrate the occurrence of natural vertical transmission of Zika virus in wild Ae. aegypti mosquitoes and suggest that this transmission mode could aid in the spread and maintenance of Zika virus in nature.

Keywords: Zika Virus; Aedes aegypti; Mosquitoes; Mexico.

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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.