Development of #American-Lineage #Influenza #H5N2 #Reassortant #Vaccine Viruses for #Pandemic #Preparedness (Viruses, abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Viruses. 2019 Jun 11;11(6). pii: E543. doi: 10.3390/v11060543.

Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness.

Chen PL1,2, Hu AY3, Lin CY4, Weng TC5, Lai CC6,7, Tseng YF8, Cheng MC9,10, Chia MY11,12, Lin WC13, Yeh CT14, Su IJ15, Lee MS16.

Author information: 1 National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. letitia@nhri.org.tw. 2 Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 30013, Taiwan. letitia@nhri.org.tw. 3 National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. alanhu@nhri.org.tw. 4 National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. grayingaries@outlook.com. 5 National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. wtc@nhri.org.tw. 6 National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. laicc2@nhri.org.tw. 7 College of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan. laicc2@nhri.org.tw. 8 National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. yufents@gmail.com. 9 Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. mccheng@mail.npust.edu.tw. 10 Animal Health Research Institutes, Danshui, New Taipei City 25158, Taiwan. mccheng@mail.npust.edu.tw. 11 National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. chiaminyuan@dragon.nchu.edu.tw. 12 Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan. chiaminyuan@dragon.nchu.edu.tw. 13 Institute of Preventive Medicine, National Defence Medical Centre, Taipei 23742, Taiwan. spps057@gmail.com. 14 Institute of Preventive Medicine, National Defence Medical Centre, Taipei 23742, Taiwan. yyhome@mail.ndmctsgh.edu.tw. 15 National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. suihjen0704@stust.edu.tw. 16 National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. minshi@nhri.org.tw.

 

Abstract

Novel low-pathogenic avian influenza (LPAI) H5N2 viruses hit poultry farms in Taiwan in 2003, and evolved into highly pathogenic avian influenza (HPAI) viruses in 2010. These viruses are reassortant viruses containing HA and NA genes from American-lineage H5N2 and six internal genes from local H6N1 viruses. According to a serological survey, the Taiwan H5N2 viruses can cause asymptomatic infections in poultry workers. Therefore, a development of influenza H5N2 vaccines is desirable for pandemic preparation. In this study, we employed reverse genetics to generate a vaccine virus having HA and NA genes from A/Chicken/CY/A2628/2012 (E7, LPAI) and six internal genes from a Vero cell-adapted high-growth H5N1 vaccine virus (Vero-15). The reassortant H5N2 vaccine virus, E7-V15, presented high-growth efficiency in Vero cells (512 HAU, 107.6 TCID50/mL), and passed all tests for qualification of candidate vaccine viruses. In ferret immunization, two doses of inactivated whole virus antigens (3 μg of HA protein) adjuvanted with alum could induce robust antibody response (HI titre 113.14). In conclusion, we have established reverse genetics to generate a qualified reassortant H5N2 vaccine virus for further development.

KEYWORDS: American-lineage H5N2 vaccine; American-lineage reassortant influenza viruses; Pandemic preparedness

PMID: 31212631 DOI: 10.3390/v11060543

Keywords: Avian Influenza; H5N1; H5N2; H6N1; Reassortant Strain; Vaccines.

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gimi69

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.

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