[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Ecol Evol. 2019 Apr 26;9(10):6148-6156. doi: 10.1002/ece3.5198. eCollection 2019 May.
Wolbachia pipientis occurs in Aedes aegypti populations in New Mexico and Florida, USA.
Kulkarni A1, Yu W1, Jiang J1, Sanchez C1, Karna AK1, Martinez KJL1, Hanley KA1, Buenemann M2, Hansen IA1, Xue RD3, Ettestad P4, Melman S4, Duguma D5, Debboun M5, Xu J1.
Author information: 1 Biology Department New Mexico State University Las Cruces New Mexico. 2 Department of Geography New Mexico State University Las Cruces New Mexico. 3 Anastasia Mosquito Control District St. Augustine Florida. 4 New Mexico Department of Health Santa Fe New Mexico. 5 Harris County Public Health Mosquito and Vector Control Division Houston Texas.
The mosquitoes Aedes aegypti (L.) and Ae. albopictus Skuse are the major vectors of dengue, Zika, yellow fever, and chikungunya viruses worldwide. Wolbachia, an endosymbiotic bacterium present in many insects, is being utilized in novel vector control strategies to manipulate mosquito life history and vector competence to curb virus transmission. Earlier studies have found that Wolbachia is commonly detected in Ae. albopictus but rarely detected in Ae. aegypti. In this study, we used a two-step PCR assay to detect Wolbachia in wild-collected samples of Ae. aegypti. The PCR products were sequenced to validate amplicons and identify Wolbachia strains. A loop-mediated isothermal amplification (LAMP) assay was developed and used for detecting Wolbachia in selected mosquito specimens as well. We found Wolbachiain 85/148 (57.4%) wild Ae. aegypti specimens from various cities in New Mexico, and in 2/46 (4.3%) from St. Augustine, Florida. Wolbachiawas not detected in 94 samples of Ae. aegypti from Deer Park, Harris County, Texas. Wolbachia detected in Ae. aegypti from both New Mexico and Florida was the wAlbB strain of Wolbachia pipientis. A Wolbachia-positive colony of Ae. aegypti was established from pupae collected in Las Cruces, New Mexico, in 2018. The infected females of this strain transmitted Wolbachia to their progeny when crossed with males of Rockefeller strain of Ae. aegypti, which does not carry Wolbachia. In contrast, none of the progeny of Las Cruces males mated to Rockefeller females were infected with Wolbachia.
KEYWORDS: Aedes aegypti; Aedes albopictus; Florida; New Mexico; Texas; Wolbachia; wAlbB
PMID: 31161026 PMCID: PMC6540660 DOI: 10.1002/ece3.5198
Keywords: Arbovirus; Mosquitoes; Aedes aegypti; Aedes albopictus; New Mexico; Florida; USA.