[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]
Emergence of carbapenem-resistant Pseudomonas asiatica producing NDM-1 and VIM-2 metallo-β-lactamases in Myanmar
Mari Tohya, Tatsuya Tada, Shin Watanabe, Kyoko Kuwahara-Arai, Khwar Nyo Zin, Ni Ni Zaw, May Yee Aung, San Mya, Khin Nyein Zan, Teruo Kirikae, Htay Htay Tin
Pseudomonas asiatica is recently proposed species of the genus Pseudomonas. This study describes eight isolates of carbapenem-resistant P. asiatica harboring blaNDM-1 and blaVIM-2, genes encoding metallo-β-lactamase (MBL). These isolates were obtained from urine samples of patients hospitalized in Myanmar. These isolates were resistant to carbapenems but susceptible to colistin. All eight isolates were positive for a carbapenemase inactivation method, CIMTrisII, and seven were positive on an immunochromatographic assay for NDM-type MBL. One isolate was highly resistant to aminoglycosides. Whole genome sequencing showed that seven isolates harbored blaNDM-1 and one harbored blaVIM-2, with these genes located on the chromosome. One isolate harbored blaNDM-1 and rmtC, a gene encoding 16S rRNA methylase. Five types of genomic environments surrounding blaNDM-1 and blaVIM-2 were detected in these eight isolates, with four isolates having the same type. These data indicate that P. asiatica harboring genes encoding carbapenemases, including blaNDM-1 and blaVIM-2, are spreading in medical settings in Myanmar.
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Keywords: Antibiotics; Drugs Resistance; Carbapenem; Beta-lactams; Aminoglycosides; Pseudomonas asiatica; Myanmar.