[Source: PLoS One, full page: (LINK). Abstract, edited.]
OPEN ACCESS / PEER-REVIEWED / RESEARCH ARTICLE
Prevalence and concentration of stx+ E. coli and E. coli O157 in bovine manure from Florida farms
Christopher A. Baker, Jaysankar De, Bruna Bertoldi, Laurel Dunn, Travis Chapin, Michele Jay-Russell, Michelle D. Danyluk, Keith R. Schneider
Published: May 24, 2019 / DOI: https://doi.org/10.1371/journal.pone.0217445
Fresh produce outbreaks due to Shiga toxin-producing Escherichia coli (STEC) continue to occur in the United States (US). Manure-amended soils can pose a public health risk when used for growing raw agricultural commodities. Knowing the prevalence and concentration of STEC in untreated biological soil amendments of animal origin (BSAAO) is important to help guide the most appropriate pre-harvest interval(s) following application to limit risks from these soil amendments. Bovine manure samples were collected from 12 farms in Florida, including samples from piles, lagoons, barns, and screened solids. Two methods were used to detect stx1/2 and rfbE genes in samples. A prevalence rate of 9% for stx1 and/or stx2 and 19% for rfbE was observed from the 518 bovine manure samples evaluated. A most probable number (MPN) assay was performed on stx+ samples when applicable. The geometric mean for stx+samples (n = 20) was 3.37 MPN g-1 (0.53 log MPN g-1) with a maximum value of 6,800 MPN g-1 (3.83 log MPN g-1). This research was part of a larger nationwide geographical study on the prevalence and concentration of STEC in bovine manure to help guide regulations on feasible pre-harvest intervals for the application of untreated BSAAO.
Citation: Baker CA, De J, Bertoldi B, Dunn L, Chapin T, Jay-Russell M, et al. (2019) Prevalence and concentration of stx+ E. coli and E. coli O157 in bovine manure from Florida farms. PLoS ONE 14(5): e0217445. https://doi.org/10.1371/journal.pone.0217445
Editor: P. Pardha-Saradhi, University of Delhi, INDIA
Received: March 18, 2019; Accepted: May 10, 2019; Published: May 24, 2019
Copyright: © 2019 Baker et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the manuscript and its Supporting Information files.
Funding: This work was supported by the Western Center for Food Safety contract U19-FD004995 from the U.S. Food and Drug Administration. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Keywords: E. Coli; Cattle; Environmental pollution; USA.