The #PA #Subunit of the #Influenza Virus #Polymerase Complex Affects #Replication and #Airborne #Transmission of the #H9N2 Subtype #Avian Influenza Virus (Viruses, abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Viruses. 2019 Jan 9;11(1). pii: E40. doi: 10.3390/v11010040.

The PA Subunit of the Influenza Virus Polymerase Complex Affects Replication and Airborne Transmission of the H9N2 Subtype Avian Influenza Virus.

Hao M1,2, Han S3,4, Meng D5,6, Li R7, Lin J8, Wang M9, Zhou T10, Chai T11.

Author information: 1 College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. mengchan1993@126.com. 2 Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University, Taian 270016, China. mengchan1993@126.com. 3 College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. 18763896230@163.com. 4 Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University, Taian 270016, China. 18763896230@163.com. 5 College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. iamli_z@126.com. 6 Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University, Taian 270016, China. iamli_z@126.com. 7 College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. lirong19900129@163.com. 8 College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. 18763806701@163.com. 9 College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. 18854937499@163.com. 10 College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. 13864453175@163.com. 11 College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China. chaitj117@163.com.

 

Abstract

The polymerase acidic (PA) protein is the third subunit of the influenza A virus polymerase. In recent years, studies have shown that PA plays an important role in overcoming the host species barrier and host adaptation of the avian influenza virus (AIV). The objective of this study was to elucidate the role of the PA subunit on the replication and airborne transmission of the H9N2 subtype AIV. By reverse genetics, a reassortant rSD01-PA was derived from the H9N2 subtype AIV A/Chicken/Shandong/01/2008 (SD01) by introducing the PA gene from the pandemic influenza A H1N1 virus A/swine/Shandong/07/2011 (SD07). Specific pathogen-free (SPF) chickens and guinea pigs were selected as the animal models for replication and aerosol transmission studies. Results show that rSD01-PA lost the ability of airborne transmission among SPF chickens because of the single substitution of the PA gene. However, rSD01-PA could infect guinea pigs through direct contact, while the parental strain SD01 could not, even though the infection of rSD01-PA could not be achieved through aerosol. In summary, our results indicate that the protein encoded by the PA gene plays a key role in replication and airborne transmission of the H9N2 subtype AIV.

KEYWORDS: H9N2 AIV; airborne transmission; pandemic 2009 H1N1 virus; reassortment; replication

PMID: 30634394 PMCID: PMC6356911 DOI: 10.3390/v11010040 [Indexed for MEDLINE]  Free PMC Article

Keywords: Avian Influenza; Swine Influenza; H1N1; H9N2; Reassortant strain.

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gimi69

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.

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