[Source: Journal of Antimicrobial Chemotherapy, full page: (LINK). Abstract, edited.]
Challenge of drug resistance in Pseudomonas aeruginosa: clonal spread of NDM-1-positive ST-308 within a tertiary hospital
Ka Lip Chew, Sophie Octavia, Oon Tek Ng, Kalisvar Marimuthu, Indumathi Venkatachalam, Bernadette Cheng, Raymond T P Lin, Jeanette W P Teo
Journal of Antimicrobial Chemotherapy, dkz169, https://doi.org/10.1093/jac/dkz169
Published: 12 May 2019
MDR Pseudomonas aeruginosa is a serious global threat to healthcare institutions. The mechanism by which drug resistance can be acquired is variable, but acquired carbapenemase production has been reported in P. aeruginosa. An investigation was performed to determine the rate and genomic epidemiology of New Delhi MBL (NDM) in β-lactam-non-susceptible isolates.
P. aeruginosa isolates from a tertiary hospital in Singapore between January 2015 and February 2018 were investigated for the presence of NDM genes.
Out of 298 pan-β-lactam-non-susceptible isolates, 31 were found to be NDM positive (10.4%). WGS demonstrated that all 31 NDM-positive isolates were clonal, belonging to ST-308. blaNDM was chromosomally inserted within an integrative and conjugative element (ICE), ICETn43716385. The NDM-P. aeruginosa isolates possessed an extensive repertoire of both cell-associated [flagella, pili, alginate/biofilm, LPS, type III secretion system (T3SS) and type VI secretion system (T6SS)] and secreted virulence factors. Antibiograms revealed higher rates of drug resistance in NDM-positive isolates compared with their non-NDM counterparts. The NDM isolates remained 100% susceptible only to colistin.
The combination of chromosomal mutations, acquired resistance genes and virulence factors likely facilitated the persistent and ongoing spread of the ST-308 clade of P. aeruginosa within the hospital. Our study illustrates the particular threat of NDM-positive P. aeruginosa in a tertiary hospital setting in the era of antimicrobial resistance.
Topic: pseudomonas aeruginosa – mutation – colistin – epidemiology – drug resistance – alginates – biofilms – bodily secretions – chromosomes – drug resistance, microbial – bacterial fimbria – flagella – genes – genome – lactams – singapore – persistence – virulence factors – antibiogram – resistance genes – type iii protein secretion system complex – whole genome sequencing
Issue Section: ORIGINAL RESEARCH
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Keywords: Antibiotics; Drugs Resistance; Beta-lactams; NDM1; Pseudomonas aeruginosa; Nosocomial outbreaks.