The Role of Pre-existing #CrossReactive Central Memory #CD4 T-Cells in #Vaccination With Previously Unseen #Influenza Strains (Front Immunol., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Front Immunol. 2019 Apr 4;10:593. doi: 10.3389/fimmu.2019.00593. eCollection 2019.

The Role of Pre-existing Cross-Reactive Central Memory CD4 T-Cells in Vaccination With Previously Unseen Influenza Strains.

Nienen M1,2,3, Stervbo U4, Mölder F5, Kaliszczyk S4, Kuchenbecker L6, Gayova L7, Schweiger B8, Jürchott K2, Hecht J9,10, Neumann AU11, Rahmann S5, Westhoff T12, Reinke P2,13, Thiel A2, Babel N2,4,13.

Author information: 1 Institute for Medical Immunology, Charité University Medicine Berlin, Berlin, Germany. 2 Berlin-Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin, Berlin, Germany. 3 Labor Berlin-Charité Vivantes GmbH, Berlin, Germany. 4 Center for Translational Medicine, Immunology and Transplantation, Marien Hospital Herne, Ruhr University Bochum, Herne, Germany. 5 Genome Informatics, Institute of Human Genetics, University Hospital Essen, University of Duisburg-Essen, Essen, Germany. 6 Applied Bioinformatics, Tübingen University, Tübingen, Germany. 7 Bogomolets National Medical University, Kyiv, Ukraine. 8 Robert-Koch Institute, Berlin, Germany. 9 Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain. 10 Universitat Pompeu Fabra (UPF), Barcelona, Spain. 11 Institute of Environmental Medicine, German Research Center for Environmental Health, Helmholtz Zentrum München, Augsburg, Germany. 12 Department of Internal Medicine, Marien Hospital Herne, Ruhr University Bochum, Herne, Germany. 13 Department of Nephrology and Intensive Care, Charité University Medicine Berlin, Berlin, Germany.



Influenza vaccination is a common approach to prevent seasonal and pandemic influenza. Pre-existing antibodies against close viral strains might impair antibody formation against previously unseen strains-a process called original antigenic sin. The role of this pre-existing cellular immunity in this process is, despite some hints from animal models, not clear. Here, we analyzed cellular and humoral immunity in healthy individuals before and after vaccination with seasonal influenza vaccine. Based on influenza-specific hemagglutination inhibiting (HI) titers, vaccinees were grouped into HI-negative and -positive cohorts followed by in-depth cytometric and TCR repertoire analysis. Both serological groups revealed cross-reactive T-cell memory to the vaccine strains at baseline that gave rise to the majority of vaccine-specific T-cells post vaccination. On the contrary, very limited number of vaccine-specific T-cell clones was recruited from the naive pool. Furthermore, baseline quantity of vaccine-specific central memory helper T-cells and clonotype richness of this population directly correlated with the vaccination efficacy. Our findings suggest that the deliberate recruitment of pre-existing cross-reactive cellular memory might help to improve vaccination outcome.

KEYWORDS: central memory T-cell; clonotype diversity; influenza vaccination; pre-existing cross-reactive T-cells; vaccination efficacy

PMID: 31019503 PMCID: PMC6458262 DOI: 10.3389/fimmu.2019.00593

Keywords: Seasonal Influenza; Vaccines; Immunology.


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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum ( in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.