[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
Sci Rep. 2019 Mar 11;9(1):4038. doi: 10.1038/s41598-019-39666-5.
RNAi-based bioinsecticide for Aedes mosquito control.
Lopez SBG1, Guimarães-Ribeiro V1, Rodriguez JVG1, Dorand FAPS1, Salles TS1, Sá-Guimarães TE1, Alvarenga ESL1, Melo ACA1,2, Almeida RV1, Moreira MF3,4.
Author information: 1 Universidade Federal do Rio de Janeiro, Departamento de Bioquímica, Instituto de Química, 21941-909, Rio de Janeiro, RJ, Brazil. 2 Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, RJ, Brazil. 3 Universidade Federal do Rio de Janeiro, Departamento de Bioquímica, Instituto de Química, 21941-909, Rio de Janeiro, RJ, Brazil. firstname.lastname@example.org. 4 Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, RJ, Brazil. email@example.com.
Zika virus infection and dengue and chikungunya fevers are emerging viral diseases that have become public health threats. Their aetiologic agents are transmitted by the bite of genus Aedes mosquitoes. Without effective therapies or vaccines, vector control is the main strategy for preventing the spread of these diseases. Increased insecticide resistance calls for biorational actions focused on control of the target vector population. The chitin required for larval survival structures is a good target for biorational control. Chitin synthases A and B (CHS) are enzymes in the chitin synthesis pathway. Double-stranded RNA (dsRNA)-mediated gene silencing (RNAi) achieves specific knockdown of target proteins. Our goal in this work, a new proposed RNAi-based bioinsecticide, was developed as a potential strategy for mosquito population control. DsRNA molecules that target five different regions in the CHSA and B transcript sequences were produced in vitro and in vivo through expression in E. coli HT115 and tested by direct addition to larval breeding water. Mature and immature larvae treated with dsRNA targeting CHS catalytic sites showed significantly decreased viability associated with a reduction in CHS transcript levels. The few larval and adult survivors displayed an altered morphology and chitin content. In association with diflubenzuron, this bioinsecticide exhibited insecticidal adjuvant properties.
PMID: 30858430 DOI: 10.1038/s41598-019-39666-5
Keywords: Arbovirus; Mosquitoes; Aedes spp.; Insecticides.