Molecular characterization of #MDR #Pseudomonas aeruginosa isolates in #hospitals in #Myanmar (Antimicrob Agents Chemother., abstract)

[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]

Molecular characterization of multidrug-resistant Pseudomonas aeruginosaisolates in hospitals in Myanmar

Tatsuya Tada, Tomomi Hishinuma, Shin Watanabe, Hiroki Uchida, Mari Tohya, Kyoko Kuwahara-Arai, San Mya, Khin Nyein Zan, Teruo Kirikae, Htay Htay Tin

DOI: 10.1128/AAC.02397-18

 

ABSTRACT

The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa has become a serious worldwide medical problem. This study was designed to clarify the genetic and epidemiological properties of MDR P. aeruginosa strains isolated from hospitals in Myanmar. Forty-five MDR P. aeruginosa isolates obtained from different patients in seven hospitals in Myanmar were screened by the broth microdilution method. The whole genomes of the MDR isolates were sequenced by MiSeq (Illumina). Phylogenetic trees were constructed from single nucleotide polymorphism concatemers. Multilocus sequence types were deduced and drug resistance genes were identified. Of the 45 isolates, 38 harbored genes encoding carbapenemases, including DIM-1, IMP-1, NDM-1, VIM-2 and VIM-5; and nine had genes encoding 16S rRNA methylases, including RmtB, RmtD3, RmtE and RmtF2. Most strains of MDR P. aeruginosaisolated in Myanmar belonged to ST1047. This is the first molecular epidemiological analysis of MDR P. aeruginosa clinical isolates in Myanmar. These findings strongly suggest that P. aeruginosa ST1047 harboring carbapenemases, including DIM-, IMP-, NDM- and VIM-type metallo-β-lactamases, have been spreading throughout medical settings in Myanmar.

Copyright © 2019 American Society for Microbiology. All Rights Reserved.

Keywords: Antibiotics; Drugs Resistance; Beta-lactams; Pseudomonas aeruginosa; Nosocomial Outbreaks; Myanmar.

—–

Published by

Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.