Polypyrimidine Tract-Binding #Protein Regulates #Enterovirus 71 #Translation Through Interaction with the Internal #Ribosomal Entry Site (Virol Sin., abstract)

[Source: Virologica Sinica, full page: (LINK). Abstract, edited.]

Polypyrimidine Tract-Binding Protein Regulates Enterovirus 71 Translation Through Interaction with the Internal Ribosomal Entry Site

Authors and affiliations: Juemin Xi 1, Fei Ye 2, Guanzhou Wang 2, Wei Han 2, Zhizhong Wei 2, Bin Yin 2, Jiangang Yuan 2, Boqin Qiang 2, Xiaozhong Peng 1, 2

1 Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, China; 2 The State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

Open Access / RESEARCH ARTICLE / First Online: 22 February 2019

 

Abstract

Enterovirus 71 (EV71), a major causative agent of hand, foot, and mouth disease, has caused periodic infection outbreaks in children in the Asia–Pacific region. In order to describe the largely unknown life cycle of EV71, the molecular basis of its virus-host interactions must first be determined. The 5′ untranslated region of EV71 contains a cloverleaf-like structure and internal ribosomal entry site (IRES), which play an important role in transcription and translation of viral protein. We found that polypyrimidine tract-binding protein 1 (PTB) bound to the IRES of EV71. RNA recognition motifs 1 and 2 of PTB were responsible for its binding to the EV71 IRES. Moreover, PTB protein was shuttled from nucleus to cytoplasm after EV71 infection. Additionally, IRES activity and viral protein production were inhibited by PTB knockdown. These results suggest that PTB interacts with the EV71 IRES, and positively regulates viral protein translation.

Keywords: EV71 – IRES – PTB – Translation

 

Electronic supplementary material

The online version of this article ( https://doi.org/10.1007/s12250-019-00089-1) contains supplementary material, which is available to authorized users.

Juemin Xi and Fei Ye contributed equally to this work.

Keywords: EV-71; HFMD.

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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.