[Source: PLoS Neglected Tropical Diseases, full page: (LINK). Abstract, edited.]
OPEN ACCESS / PEER-REVIEWED / RESEARCH ARTICLE
Anopheles mosquitoes may drive invasion and transmission of Mayaro virus across geographically diverse regions
Marco Brustolin , Sujit Pujhari , Cory A. Henderson, Jason L. Rasgon
Published: November 7, 2018 / DOI: https://doi.org/10.1371/journal.pntd.0006895 / This is an uncorrected proof.
The Togavirus (Alphavirus) Mayaro virus (MAYV) was initially described in 1954 from Mayaro County (Trinidad) and has been responsible for outbreaks in South America and the Caribbean. Imported MAYV cases are on the rise, leading to invasion concerns similar to Chikungunya and Zika viruses. Little is known about the range of mosquito species that are competent MAYV vectors. We tested vector competence of 2 MAYV genotypes in laboratory strains of six mosquito species (Aedes aegypti, Anopheles freeborni, An. gambiae, An. quadrimaculatus, An. stephensi, Culex quinquefasciatus). Ae. aegypti and Cx. quinquefasciatus were poor MAYV vectors, and had either poor or null infection and transmission rates at the tested viral challenge titers. In contrast, all Anopheles species were able to transmit MAYV, and 3 of the 4 species transmitted both genotypes. The Anopheles species tested are divergent and native to widely separated geographic regions (Africa, Asia, North America), suggesting that Anopheles may be important in the invasion and spread of MAYV across diverse regions of the world.
Mayaro virus (MAYV) is a mosquito-borne Alphavirus responsible for outbreaks in South America and the Caribbean. In this study we infected different species of mosquito (belonging to the genera Aedes, Anopheles and Culex) with MAYV and tested their capacity to transmit the virus at different time points. Results show that Anopheles mosquitoes were competent vectors for 2 genotypes of MAYV, while Aedes and Culex were poor vectors. The capacity of Anopheles mosquitoes to transmit MAYV highlights their importance as neglected vectors of arboviruses. These data suggest that Anopheles mosquitoes have the potential to sustain transmission cycles of neglected pathogens in naïve regions, including the United States.
Citation: Brustolin M, Pujhari S, Henderson CA, Rasgon JL (2018) Anophelesmosquitoes may drive invasion and transmission of Mayaro virus across geographically diverse regions. PLoS Negl Trop Dis 12(11): e0006895. https://doi.org/10.1371/journal.pntd.0006895
Editor: Rebecca C. Christofferson, Louisiana State University, UNITED STATES
Received: July 3, 2018; Accepted: October 3, 2018; Published: November 7, 2018
Copyright: © 2018 Brustolin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All raw data is provided as Supplementary material and in Table 1
Funding: This research was supported by the National Institutes of Health (nih.gov) grants R01AI116636, R01AI128201, and R21AI128918. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Keywords: Alphavirus; Togavirus; Mayaro Virus; Mosquitoes; Anopheles spp.