Prevalence of #mcr-type genes among #colistin-resistant #Enterobacteriaceae collected in 2014-2016 as part of the #INFORM global #surveillance program (PLoS One, abstract)

[Source: PLoS One, full page: (LINK). Abstract, edited.]

OPEN ACCESS /  PEER-REVIEWED / RESEARCH ARTICLE

Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program

Mark G. Wise , Mark A. Estabrook, Daniel F. Sahm, Gregory G. Stone, Krystyna M. Kazmierczak

Published: April 2, 2018 / DOI: https://doi.org/10.1371/journal.pone.0195281

 

Abstract

A set of 908 clinically derived colistin-resistant Enterobacteriaeae isolates collected worldwide in 2014–2016 were screened for the presence of the plasmid-borne mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 genes. In total 3.2% (29/908) of the collection were positive for mcr, including 27 Escherichia coli, 1 Klebsiella pneumoniae and 1 Enterobacter cloacae. Twenty-four isolates possessed genes from the mcr-1 family, including the original mcr-1 (n = 22), as well as mcr-1.2 (n = 1) and mcr-1.5 (n = 1), which each differ from mcr-1 by encoding single amino acid variations. Genes from the mcr-3 family were found in isolates from Thailand, including mcr-3.1 (n = 3) and mcr-3.2 (n = 1). An E. coli isolated from a patient with a urinary tract infection in Colombia contained the recently discovered mcr-5. The full colistin-resistant collection was tested against a panel of antimicrobial agents with ceftazidime-avibactam and tigecycline exhibiting the highest activity.

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Citation: Wise MG, Estabrook MA, Sahm DF, Stone GG, Kazmierczak KM (2018) Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program. PLoS ONE 13(4): e0195281. https://doi.org/10.1371/journal.pone.0195281

Editor: Yung-Fu Chang, Cornell University, UNITED STATES

Received: December 13, 2017; Accepted: March 19, 2018; Published: April 2, 2018

Copyright: © 2018 Wise et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are contained within the manuscript.

Funding: All work was performed at IHMA and sponsored by AstraZeneca Pharmaceuticals, LP, which also included compensation fees for manuscript preparation. AstraZeneca’s rights to ceftazidime-avibactam were acquired by Pfizer in December 2016.

Competing interests: All work was performed at IHMA and sponsored by AstraZeneca Pharmaceuticals, LP, which also included compensation fees for manuscript preparation. AstraZeneca’s rights to ceftazidime-avibactam were acquired by Pfizer in December 2016. G.S. was an employee of and shareholder in AstraZeneca Pharmaceuticals at the time of the study, and is currently an employee of Pfizer. M.W., M.E., D.S. and K.K. are employees of IHMA which received funding from AstraZeneca for the conduct of the study and development of this manuscript. None of the IHMA authors have any personal financial interest in the study sponsor (AstraZeneca Pharmaceuticals, LP) to disclose. This does not alter our adherence to all PLOS ONE policies on sharing data and materials.

Keywords: Antibiotics; Drugs Resistance; Colistin; Enterobacteriaceae; Ceftazidime-Avibactam; Tigecycline.

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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.